Rapid determination of total aflatoxins and ochratoxins A in meat products by immuno-affinity fluorimetry

Selective tools for the solid-phase extraction of Ochratoxin A from various complex samples: immunosorbents, oligosorbents, and molecularly imprinted polymers

“…ISs have largely been reported for the purification of OTA from cereal extracts [9, 16-19, 22, 24-26] but also for the selective treatment of other types of samples such as coffee and related products [10,14,16,21,25,27], milk [15], cocoa and related products [16,23], wine [7,8,12,28], beer [12], grape, grape wine fruit or winery products [20], meat or animal products [29], spices, oil, nuts or infant formula [16]. They were also applied to the extraction of OTA from biological fluids such as serum, plasma [15,30,31] and urine [11,13].…”

“…In most of the cases, the main objective was to only extract OTA from real samples before its analysis by HPLC/Fluo [9,10,13,14,18,20,21,27,28,31] or by ELISA [7]. Some works also reported the simultaneous extraction of OTA and aflatoxins [8,16,29,32] by using a cartridge containing both anti-OTA and antiaflatoxins antibodies or using two cartridges in tandem. The same approach was proposed for the simultaneous determination of OTA and zearalenone [24], five [17] or ten [22] other mycotoxins, the last approaches implying the use of LC/MS analysis for the simultaneous determination of the whole mycotoxins in the same samples.…”

Novel fluoroimmunoassays for detecting ochratoxin A using CdTe quantum dots

“…Current determination of OTA was primarily achieved by instrumental analysis [5][6][7][8], enzymelinked immunosorbent assay (ELISA) [9][10][11] and colloidal gold immunochromatographic assay [9,[12][13]. ELISA had been developed rapidly in the past years because of their simplicity, adaptability, sensitivity and selectivity.…”

Simple and sensitive detection of aflatoxin B1 within five minute using a non-conventional competitive immunosensing mode

“…Aflatoxins, a group of natural food toxins which are recognized as toxic and carcinogenic secondary metabolites, are mainly produced by certain strains of Aspergillus flavus, Aspergillus paraciticus and Aspergillus nomius (Masood et al, 2015). Since Aspergillus molds could grow on crops pre-, during, and post-harvest and their toxic metabolites are very stable to chemical and physical stresses, aflatoxins have been found in raw and processed materials and represent the most common cause of chemical contamination of foodstuffs (Abd-Elghany and Sallam, 2015;Zivoli et al, 2014). Among 20 types of aflatoxins, 4 types of aflatoxins including B 1 , B 2 , G 1 and G 2 commonly occur in the natural environment .…”