Chlorpyrifos induced testicular damage in rats: Ameliorative effect of glutathione antioxidant

Elsharkawy, Eman E.; Yahia, Doha; El-Nisr, Neveen A.

doi:10.1002/tox.21831

Cited by 21 publications

(13 citation statements)

References 44 publications

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“…In agreement with previous studies, our histopathological examination of the testicular tissue revealed significant decline in the number of spermatogenic cells, Sertoli cells and inter‐tubular blood cells in CPF‐exposed groups (Dutta & Sahu, ; Elsharkawy et al, ; Mandal & Das, ; Mosbah et al, ; Sai et al, ). Among these cells, the mean spermatocyte and spermatid counts were significantly increased by some, and not all, treatment regimens in exercise‐trained groups when compared with the similarly treated sedentary animals (Figure e,f).…”

Section: Discussionsupporting

confidence: 92%

“…Some previous research works have shown that CPF administration leads to drastic histo‐morphologic changes in the testes of animal models, such as testicular atrophy, shrinkage of the seminiferous tubules, tubular diameter reduction and degenerative changes in the germinal epithelium (Dutta & Sahu, ; Elsharkawy, Yahia, & El‐Nisr, ; Farag et al, ; Mosbah, Yousef, Maranghi, & Mantovani, ). However, other studies have failed to detect some or all of these damages (Joshi et al, ; Kheradmandi, Jorsaraei, Feizi, Moghadamnia, & Neamati, ; Sai et al, ; Wen, Wei, & Pan, ).…”

Section: Discussionmentioning

confidence: 99%

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Investigating the protective effect of aerobic exercise on oxidative stress and histological damages of testicular tissue associated with chlorpyrifos in male rats

et al. 2019

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The current study aimed to investigate the protective effects of moderate aerobic exercise against chlorpyrifos (CPF)‐induced testes dysfunction. In excremental study, 48 adult male albino rats were randomly allocated into 16 groups of 3 rats each. Twelve experimental groups received intraperitoneal injection (5 days a week) of either 1.0 or 3.0 mg/kg body weight CPF in DMSO for 2, 4 or 6 consecutive weeks. Seven of these experimental groups were subjected to run at moderate exercise intensity for 5 days per week over 2 weeks, whereas the other groups were not. Two groups (sham groups) were administered to the equal volume of vehicle (DMSO) for 4 or 6 consecutive weeks. The remaining two groups comprised the control groups including a sedentary and an exercise‐trained control group. Exercise training leads to a markedly increase in testicular superoxide dismutase (SOD) activity in CPF‐exposed rats compared with corresponding sedentary animals (p < .05). Lipid peroxidation level was found to be significantly decreased in the testis of exercised animals that had been exposed to CPF (p < .05). Our results suggest that aerobic exercise can alleviate the oxidative stress induced by sub‐acute CPF exposure in testis. Exercise training could barely mitigate CPF‐induced testicular damages in rats.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Investigating the protective effect of aerobic exercise on oxidative stress and histological damages of testicular tissue associated with chlorpyrifos in male rats

et al. 2019

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“…Thus, spermatogenesis and sperm function must be protected from oxidative stress. To avoid harmful effects from ROS, the testes mainly use enzymatic copper/zinc superoxide dismutase (Cu/Zn-SOD or SOD1), mitochondrial SOD (Fe/Mn-SOD or SOD2), extracellular SOD (SOD-Ex or SOD3) [Bauche et al 1994;Gu and Hecht 1996;Mruk et al 2002], catalase (CAT) [Dastig et al 2011;Luers et al 2006;Nenicu et al 2007], and selenoenzyme phospholipid hydroperoxide glutathione peroxidase [Baek et al 2007;Kaur et al 2006] and non-enzymatic antioxidants, like reduced glutathione (GSH) [Bilommi et al 2013;Elsharkawy et al 2012]. The cytoplasmic space of sperm is limited and the availability and location of intracellular antioxidant enzymes is restricted, which may be further compromised by the effects of oxidative stress [Weir and Robaire 2007].…”

Section: Introductionmentioning

confidence: 99%

Oxidative status in testis and epididymal sperm parameters after acute and chronic stress by cold-water immersion in the adult rat

García-Díaz

Gómez-Quiroz

Arenas‐Ríos

et al. 2015

Systems Biology in Reproductive Medicine

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Stress is associated with detrimental effects on male reproductive function. It is known that stress increases reactive oxygen species (ROS) generation in the male reproductive tract. High ROS levels may be linked to low sperm quality and male infertility. However, it is still not clear if ROS are generated by stress in the testis. The objective of this study was to characterize the role of oxidative stress induced by cold-water immersion stress in the testis of adult male rats and its relation with alterations in cauda epididymal sperm. Adult male rats were exposed to acute stress or chronic stress by cold-water immersion. Rats were sacrificed at 0, 6, 12, and 24 hours immediately following acute stress exposure, and after 20, 40, and 50 days of chronic stress. ROS production increased only at 6 hours post-stress, while the activity and expression of antioxidant enzymes, lipid peroxidation (LPO), and sperm parameters were not modified in the testis. Corticosterone increased immediately after acute stress, whereas testosterone was not modified. After chronic stress, testicular absolute weight decreased; in addition, ROS production and LPO increased at 20, 40, and 50 days. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) decreased throughout the duration of chronic stress and the activity of catalase (CAT) decreased at 40 and 50 days, and increased at 20 days. The expression of copper/zinc superoxide dismutase (SOD1) and CAT were not modified, but the expression of phospholipid hydroperoxide glutathione peroxidase (GPx-4) decreased at 20 days. Motility, viability, and sperm count decreased, while abnormal sperm increased with chronic stress. These results suggest that during acute stress there is a redox state regulation in the testis since no deleterious effect was observed. In contrast, equilibrium redox is lost during chronic stress, with low enzyme activity but without modifying their expression. In addition, corticosterone increased while testosterone decreased, this decrease is related to the negative effects seen in sperm.

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“…10,11 Generally, lipid peroxidation (LPO) due to OP poisoning is evidenced by enhanced levels of thiobarbituric acid reactive substances (TBARS) in the plasma, brain, lung, testes, and erythrocytes. [12][13][14][15] The extensive and indiscriminate use of OP pesticides in agriculture has been a major concern because of its potentially known or suspected harmful effects to humans. 4 Moreover, some of these pesticides remain in the soil, ground water, drinking water, marine organisms, and various food products.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A model of chlorpyrifos distribution and its biochemical effects on the liver and kidneys of rats

et al. 2016

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This study investigated the main target sites of chlorpyrifos (CPF), its effect on biochemical indices, and the pathological changes observed in rat liver and kidney function using gas chromatography/mass spectrometry. Adult female Wistar rats ( n = 12) were randomly assigned into two groups (one control and one test group; n = 6 each). The test group received CPF via oral gavage for 21 days at 5 mg/kg daily. The distribution of CPF was determined in various organs (liver, brain, heart, lung, kidney, ovary, adipose tissue, and skeletal muscle), urine and stool samples using GCMS. Approximately 6.18% of CPF was distributed in the body tissues, and the highest CPF concentration (3.80%) was found in adipose tissue. CPF also accumulated in the liver (0.29%), brain (0.22%), kidney (0.10%), and ovary (0.03%). Approximately 83.60% of CPF was detected in the urine. CPF exposure resulted in a significant increase in plasma transaminases, alkaline phosphatase, and total bilirubin levels, a significant reduction in total protein levels and an altered lipid profile. Oxidative stress due to CPF administration was also evidenced by a significant increase in liver malondialdehyde levels. The detrimental effects of CPF on kidney function consisted of a significant increase in plasma urea and creatinine levels. Liver and kidney histology confirmed the observed biochemical changes. In conclusion, CPF bioaccumulates over time and exerts toxic effects on animals.

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Chlorpyrifos induced testicular damage in rats: Ameliorative effect of glutathione antioxidant

Cited by 21 publications

References 44 publications

Investigating the protective effect of aerobic exercise on oxidative stress and histological damages of testicular tissue associated with chlorpyrifos in male rats

Investigating the protective effect of aerobic exercise on oxidative stress and histological damages of testicular tissue associated with chlorpyrifos in male rats

Oxidative status in testis and epididymal sperm parameters after acute and chronic stress by cold-water immersion in the adult rat

A model of chlorpyrifos distribution and its biochemical effects on the liver and kidneys of rats

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