Chlorpyrifos induced reproductive toxicity in male mice

Farag, Ahmed A.; Radwan, Amany H.; Sorour, Fardous; Okazy, Ahmed El; El-Agamy, El-Sayed I.; El-Sebae, Abd El-Khaliek

doi:10.1016/j.reprotox.2009.10.003

Cited by 90 publications

(44 citation statements)

References 43 publications

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“…This inhibition leads to the accumulation of the neurotransmitter acetylcholine in nerves which interferes with muscular activities and the functioning of vital organs, produces serious symptoms, and may even lead to death [13,14]. Target site of other various pesticides are-human erythrocytes [15], reproduction and development system [16], endocrine system [17], liver, central nervous system, and immune system [18,19], etc. Thus, the analysis of pesticide residues is an important concern due to their bioaccumulation effect, high toxicity and their long-term damage risk to both the environment and lives even though their concentration is very low.…”

Section: Introductionmentioning

confidence: 99%

Biosensor Technology for Pesticides—A review

Verma

Bhardwaj

2015

Appl Biochem Biotechnol

243

139

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Pesticides, due to their lucrative outcomes, are majorly implicated in agricultural fields for crop production enhancement. Due to their pest removal properties, pesticides of various classes have been designed to persist in the environment over a longer duration after their application to achieve maximum effectiveness. Apart from their recalcitrant structure and agricultural benefits, pesticides also impose acute toxicological effects onto the other various life forms. Their accumulation in the living system may prove to be detrimental if established in higher concentrations. Thus, their prompt and accurate analysis is a crucial matter of concern. Conventional techniques like chromatographic techniques (HPLC, GC, etc.) used for pesticides detection are associated with various limitations like stumpy sensitivity and efficiency, time consumption, laboriousity, requirement of expensive equipments and highly trained technicians, and many more. So there is a need to recruit the methods which can detect these neurotoxic compounds sensitively, selectively, rapidly, and easily in the field. Present work is a brief review of the pesticide effects, their current usage scenario, permissible limits in various food stuffs and 21st century advancements of biosensor technology for pesticide detection. Due to their exceptional performance capabilities, easiness in operation and on-site working, numerous biosensors have been developed for bio-monitoring of various environmental samples for pesticide evaluation immensely throughout the globe. Till date, based on sensing element (enzyme based, antibody based, etc.) and type of detection method used (Electrochemical, optical, and piezoelectric, etc.), a number of biosensors have been developed for pesticide detection. In present communication, authors have summarized 21st century's approaches of biosensor technology for pesticide detection such as enzyme-based biosensors, immunosensors, aptamers, molecularly imprinted polymers, and biochips technology. Also, the major technological advancements of nanotechnology in the field of biosensor technology are discussed. Various biosensors mentioned in manuscript are found to exhibit storage stability of biocomponent ranging from 30-60 days, detection limit of 10(-6) - 10(-16) M, response time of 1-20 min and applications of developed biosensors in environmental samples (water, food, vegetables, milk, and juice samples, etc.) are also discussed. Researchers all over the globe are working towards the development of different biosensing techniques based on contrast approaches for the detection of pesticides in various environmental samples.

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Section: Introductionmentioning

confidence: 99%

Biosensor Technology for Pesticides—A review

Verma

Bhardwaj

2015

Appl Biochem Biotechnol

243

139

View full text Add to dashboard Cite

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“…Their functional activity is regulated by the luteinizing hormone (LH), which binds to LH receptors in the Leydig plasma membrane (Catt and Dufau, 1976). Testosterone secreted by the Leydig cells is essential for normal spermatogenesis and fertility (Farag et al, 2010). Degenerations of interstitial cells of Leydig in Banded Gourami testes due to different concentrations of chlorpyrifos were evident in the present study.…”

Section: Effects Of Chlorpyrifos On Testessupporting

confidence: 55%

Effects of insecticides on aquatic ecosystems in Bangladesh

Sumon

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“…The increase observed in follicle stimulating hormone concentration could therefore be ascribed to the feedback on the hormone due to loss of germ and Sertoli cells caused by long term administration of proguanil. Spermatogenesis and fertility are critically dependent upon the maintenance of adequate levels of testosterone (Farag et al, 2010). In this study, serum testosterone concentration was significantly decreased both at short and long term administration.…”

Section: Discussionmentioning

confidence: 56%

“…This could be linked with the testicular and epididymal damage reported in this study. In a similar fi nding, chlorpyrifos was reported to cause severe testicular damage which resulted in reduction in sperm count and thus fertility in male mice (Farag et al, 2010;Joshi et al, 2007).…”

Section: Discussionmentioning

confidence: 82%

Prolonged administration of proguanil induces reproductive toxicity in male rats

Stephen

Raji

2011

J. Toxicol. Sci.

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-The toxicity of proguanil was studied on reproductive activities in male rats using in vivo and in vitro methods. Groups of ten to twelve-week-old rats were administered proguanil (2.9 mg/kg body weight) daily for 5 days and 6 weeks respectively. Thereafter, body and reproductive organ weights were taken, sperm parameters were analyzed, while the histology of the testis and epididymis were carried out. Also, serum levels of testosterone, luteinizing hormone and follicle stimulating hormone were determined. Sertoli cells obtained from sixteen to eighteen day-old-rats were cultured and treated with 0.3 μM to 10 μM of proguanil for 5 days after which Sertoli cell viability and nuclei integrity were determined. Also, the genetic expressions of transferrin and Glial cell line-derived neurotrophic factor (G.D.N.F.) were assessed. The data obtained were analyzed using the analysis of variance (ANOVA) with Students-Newman-Keuls multiple comparison test. The results showed duration dependent significant decrease in body and organ weights and also in sperm parameters. Serum testosterone level was significantly decreased while luteinizing hormone and follicle stimulating hormone showed no significant change. Duration dependent degeneration was also observed in the testis and epididymis of treated rats. These changes were restored in recovery experiment. Viability and DNA integrity of treated Sertoli cells reduced in a dose and duration dependent manner. However, the genetic expressions of transferrin and Glial cell linederived neurotropic factor were normal. These results show that proguanil could induce infertility in rats which may act by distorting the blood-testis barrier formed by the Sertoli cells.

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Chlorpyrifos induced reproductive toxicity in male mice

Cited by 90 publications

References 43 publications

Biosensor Technology for Pesticides—A review

Biosensor Technology for Pesticides—A review

Effects of insecticides on aquatic ecosystems in Bangladesh

Prolonged administration of proguanil induces reproductive toxicity in male rats

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