1974
DOI: 10.1083/jcb.63.1.160
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CHLOROPLAST RIBOSOME BIOGENESIS IN CHLAMYDOMONAS

Abstract: Chloroplast protein synthesis in Chlarnydomonas reinhardtii is dispensable when cells are provided acetate as a carbon source. Mutants defective in synthesis, assembly, or function of chloroplast ribosomes are therefore conditionally viable.

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Cited by 67 publications
(40 citation statements)
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References 42 publications
(35 reference statements)
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“…Third, the heteroplasmic orf722 mutant has a growth phenotype similar to that of previously characterized mutants with decreased chloroplast ribosome functions. These mutants grow at or near wild-type rate under heterotrophic or mixotrophic conditions, but are unable to grow under phototrophic conditions [20,22]. This is not surprising, considering that chloroplast ribosomes synthe- size a large number of chloroplast-encoded proteins that are required in photosynthesis (e.g.…”
Section: Discussionmentioning
confidence: 96%
See 1 more Smart Citation
“…Third, the heteroplasmic orf722 mutant has a growth phenotype similar to that of previously characterized mutants with decreased chloroplast ribosome functions. These mutants grow at or near wild-type rate under heterotrophic or mixotrophic conditions, but are unable to grow under phototrophic conditions [20,22]. This is not surprising, considering that chloroplast ribosomes synthe- size a large number of chloroplast-encoded proteins that are required in photosynthesis (e.g.…”
Section: Discussionmentioning
confidence: 96%
“…Second, Orff 12 is functionally essential for cell growth, just as expected if it is a ribosomal protein. In Chlamydomonas, chloroplast ribosome function is essential for cell viability, although it can be reduced to a minimal level required for cell growth under heterotrophic or mixotrophic conditions [5,19,20]. This is presumably due to a necessity to make one or more chloroplast-encoded proteins that have essential cellular functions, as has been suggested for plants [21].…”
Section: Discussionmentioning
confidence: 99%
“…The mutations are unlinked (3), exhibit Mendelian inheritance (3), and affect distinct steps of chloroplast ribosome assembly (14). Whereas the ac2O mutation apparently disrupts the formation of both 41 S and 54S ribosomal subunits, crl acts on the 41S subunit alone (14).…”
Section: Resultsmentioning
confidence: 99%
“…The mutations are unlinked (3), exhibit Mendelian inheritance (3), and affect distinct steps of chloroplast ribosome assembly (14). Whereas the ac2O mutation apparently disrupts the formation of both 41 S and 54S ribosomal subunits, crl acts on the 41S subunit alone (14). The primary lesion in these cells, markedly reduced levels of chloroplast ribosomes (14,24), leads to various functional and structural deficiencies whose cause, in some cases, has been traced to the lack of chloroplast-synthesized gene products.…”
Section: Resultsmentioning
confidence: 99%
“…Screening for mutants with enhanced chlorophyll fluorescence (5) (6,7). The shortcomings of the arsenate procedure include an unknown mode of action and the prevalence of arsenate permeability mutants among the recovered strains (8). Because mutants that are blocked in photosynthetic CO2 fixation are also isolated (7,8), arsenate resistance is not a property restricted to those with impaired photosynthetic electron transport.…”
mentioning
confidence: 99%