1993
DOI: 10.1016/0014-5793(93)80808-8
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The unusual rps3‐like orf712 is functionally essential and structurally conserved in Chlamydomonas

Abstract: The Chlumydomonas reinhardtii chloroplast orf712 is a previously described open reading frame that lacks a detectable transcript but potentially encodes a polypeptide with sequence similarities to ribosomal protein Rps3 only at its N-and C-termini. Here we report that orj712 is an essential gene, as demonstrated through gene disruption by particle gun-mediated chloroplast transformation. We also show that an orf712 is present and structurally conserved in all of the two or three major Chlumydomonus lineages. O… Show more

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Cited by 15 publications
(9 citation statements)
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References 23 publications
(21 reference statements)
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“…The Chlamydomonas chloroplast orf712 gene has been reported to encode an unusual S3-like protein (Fong and Surzycki, 1992;Liu et al, 1993;Turmel and Otis, 1994). The predicted amino acid sequence of ORF712 shows significant similarity to that of bacterial and chloroplast ribosomal protein S3, but the similarity is limited to the N and C termini of the ORF.…”
Section: S3 Homologmentioning
confidence: 96%
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“…The Chlamydomonas chloroplast orf712 gene has been reported to encode an unusual S3-like protein (Fong and Surzycki, 1992;Liu et al, 1993;Turmel and Otis, 1994). The predicted amino acid sequence of ORF712 shows significant similarity to that of bacterial and chloroplast ribosomal protein S3, but the similarity is limited to the N and C termini of the ORF.…”
Section: S3 Homologmentioning
confidence: 96%
“…An INS of 472 amino acids, lacking similarity to other known proteins, is found in ORF712. The orf712 gene is reported to be essential for cell growth and is conserved in the Chlamydomonas lineage (Liu et al, 1993). Thirteen peptides obtained from several gel sections, mainly from section 3 containing a 76-kD protein, matched the orf712 protein product, identifying this gene as prps3.…”
Section: S3 Homologmentioning
confidence: 98%
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“…Any chloroplast gene can be inactivated or modified and the resulting strain evaluated for photosynthetic function, assembly of thylakoid membrane and multisubunit complexes, and the transcription and translation of specific plastid genes. Mutagenesis of specific open reading frames on the chloroplast chromosome has identified genes important for maintaining photosynthetic activity and the assembly of the photosynthetic apparatus, as well as genes essential for viability (Goldschmidt-Clermont 1991, Liu et al 1993, Huang et al 1994, Monod et al 1994, Xie and Merchant 1996, Boudreau et al 1997a, b, Rolland et al 1997. Chloroplast transformation has allowed investigators to use site-directed mutagenesis to determine structure-function relationships for specific chloroplast proteins (Hallahan et al 1995, Whitelegge et al 1995, Fischer et al 1996, Webber et al 1996, Lardans et al 1997, Melkozernov et al 1997, Xiong et al 1997.…”
Section: Chlamydomonas As a Modelmentioning
confidence: 99%
“…In most algae and land plants the rps3 gene is uninterrupted, but in Euglena gracilis it contains two introns [55]. In the chloroplast genome of the Chlamydomonas species there is no gene equivalent to rsp3 in the expected region between rpl22 and rpl16 [56]. Two or more representatives of each type (A, N, E, C, M) have been sequenced from the L14 family.…”
Section: Research Articlesmentioning
confidence: 99%