Chloroplast proteome response to drought stress and recovery in tomato (Solanum lycopersicum L.)

Tamburino, Rachele; Vitale, Monica; Ruggiero, Alessandra; Sassi, Mauro; Sannino, Lorenza; Arena, Simona; Costa, Antonello; Batelli, Giorgia; Zambrano, Nicola; Scaloni, Andrea; Grillo, Stefania; Scotti, Nunzia

doi:10.1186/s12870-017-0971-0

Cited by 116 publications

(75 citation statements)

References 84 publications

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“…Crop Breeding, Genetics and Genomics 26 of 32 regulated for both genotypes (Figure 2). This protein was also reported as down-regulated in tomato plants [40] under stress drought.…”

Section: Discussionmentioning

confidence: 73%

Proteomic and Metabolomic Analysis of a Drought Tolerant Soybean Cultivar from Brazilian Savanna

2019

Crop Breed Genet Genom.

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Drought is the major abiotic stress which contributes to the reduction in the productivity of soybeans in Brazil, especially in the savanna regions. Two parental genotypes contrasting in drought tolerance (Embrapa 48tolerant and BR 16-sensitive) were used to study the molecular mechanism underlying this process in soybeans. The hydric potential of the BR 16 reached values of −1.0 and −1.5 MPa, three days before the Embrapa 48, confirming the contrasting drought tolerance. The proteomic, phosphoproteomic and metabolomic profiles were evaluated to detect the metabolic pathways, which were affected by the drought stress. An integrative overview showed that the tolerant plants maintain cell homeostasis and the photosynthetic metabolism was unchanged under the stress condition in contrast to the sensitive genotype that showed several dysregulated pathways. These findings were confirmed by the protein expression and protein regulation by phosphorylation. Furthermore, just small deviations in the metabolic pathways were observed for drought-tolerant plants in comparison to the sensitive genotype. Complex post-translational modification patterns by phosphorylation were detected in some key enzymes, such as carbonic

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“…Crop Breeding, Genetics and Genomics 26 of 32 regulated for both genotypes (Figure 2). This protein was also reported as down-regulated in tomato plants [40] under stress drought.…”

Section: Discussionmentioning

confidence: 73%

Proteomic and Metabolomic Analysis of a Drought Tolerant Soybean Cultivar from Brazilian Savanna

2019

Crop Breed Genet Genom.

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“…However, a decreased relative abundance of CB proteins was recorded in soybean seedlings under flooding stress (Hossain and Komatsu 2014). Furthermore, a decreased content of proteins associated with photosynthesis including photosystem components, such as CB protein in tomato (Tamburino et al 2017), and rice (Wang et al 2011), were observed in response to drought stress. Overall, a reduced photosynthesis activity and a low relative abundance of CB proteins lead to suppression of seedling growth under waterlogging stress.…”

Section: Alteration In Leaf Proteome Under Waterlogging Stressmentioning

confidence: 96%

Proteome analysis of sesame leaves in response to waterlogging stress at vegetative and flowering stages

Jung¹,

Roy²,

Cho³

et al. 2019

Biologia plant.

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Waterlogging, a major environmental stress, impairs plant growth and development and induces synthesis of different proteins. To understand the molecular mechanisms coupled with morpho-physiological alterations underlying waterlogging tolerance, the LTQ-FTICR MS/MS technique was employed to map the proteomes of leaves of sesame grown under control and waterlogged conditions. The waterlogging treatment caused dramatic alterations in morphological and biochemical properties of the leaves of sesame. For proteome analysis, more than 75 reproducible protein spots were identified on 2-DE gels wherein 51 protein spots (≥ 1.5-fold change) were used for analysis by mass spectrometry. Among 51 differentially abundant proteins, 20 were specific to the 10-leaf stage and 31 were specific to the flowering stage. Most of the differentially abundant proteins were involved in group metabolism, and energy and stress defense. Oxygen-evolving enhancer protein 1, ATP synthase subunit, heat shock proteins, glutamine synthetase, glyceraldehyde-3-phosphate dehydrogenase, and superoxide dismutase were upregulated under waterlogging. However, the photosynthesis-and protein biosynthesisrelated proteins (e.g., ribulose-1,5-bisphosphate carboxylase/oxygenase activase, and S-adenosylmethionine synthase 1) were down-regulated under waterlogging. The protein interaction network indicates that energy metabolism-and stressand defense-related proteins were involved in the protein-protein interaction network, which could form an indispensable network in sesame leaves. To this end, physiological results highlighted the impairment of photosyntheis, which is consistent with results obtained at the proteome level. The upregulation of metabolism-, energy-, and stress defenserelated proteins in response to waterlogging stress may provide new insights into the complex mechanisms underlying waterlogging tolerance in sesame.

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“…Pure and intact wheat chloroplasts were isolated from 10 g of fresh leaves according to Tamburino et al with modifications [10]. All steps were carried out at 4°C.…”

Section: Chloroplast Isolationmentioning

confidence: 99%

“…A proteomic analysis following chloroplast isolation provides a promising approach for elucidating chloroplast-located protein regulation and its relationship with stress resistance [7,8]. Until now, the strategy of chloroplast proteomics has been employed to investigate the possible molecular regulatory pathways in response to biotic or abiotic stress in a variety of plants, such as the chlorosis mechanisms of Nicotiana tabacum leaves induced by viral infection [9], the response mechanism of tomato (Solanum lycopersicum L.) to drought stress and recovery [10], and the pathways associated with salt tolerance in Kandelia candel [11]. However, to the best of our knowledge, few studies have reported on the chloroplast proteomic analysis of wheat in response to drought stress, especially when an exogenous regulator is applied.…”

Section: Introductionmentioning

confidence: 99%

The iTRAQ-based chloroplast proteomic analysis of Triticum aestivum L. leaves subjected to drought stress and 5-aminolevulinic acid alleviation reveals several proteins involved in the protection of photosynthesis

Wang

Liu

et al. 2020

BMC Plant Biol

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Backgrounds: The perturbance of chloroplast proteins is a major cause of photosynthesis inhibition under drought stress. The exogenous application of 5-aminolevulinic acid (ALA) mitigates the damage caused by drought stress, protecting plant growth and development, but the regulatory mechanism behind this process remains obscure.Results: Wheat seedlings were drought treated, and the iTRAQ-based proteomic approach was employed to assess the difference in chloroplast protein content caused by exogenous ALA. A total of 9499 peptides, which could be classified into 2442 protein groups, were identified with ≤0.01 FDR. Moreover, the contents of 87 chloroplast proteins was changed by drought stress alone compared to that of the drought-free control, while the contents of 469 was changed by exogenous ALA application under drought stress compared to that of drought stress alone. The Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis results suggested that the ALA pretreatment adjusted some biological pathways, such as metabolic pathways and pathways involved in photosynthesis and ribosomes, to enhance the drought resistance of chloroplasts. Furthermore, the drought-promoted H 2 O 2 accumulation and O 2 − production in chloroplasts were alleviated by the exogenous pretreatment of ALA, while peroxidase (POD) and glutathione peroxidase (GPX) activities were upregulated, which agreed with the chloroplast proteomic data. We suggested that ALA promoted reactive oxygen species (ROS) scavenging in chloroplasts by regulating enzymatic processes. Conclusions:Our results from chloroplast proteomics extend the understanding of the mechanisms employed by exogenous ALA to defend against drought stress in wheat.

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Chloroplast proteome response to drought stress and recovery in tomato (Solanum lycopersicum L.)

Cited by 116 publications

References 84 publications

Proteomic and Metabolomic Analysis of a Drought Tolerant Soybean Cultivar from Brazilian Savanna

Proteomic and Metabolomic Analysis of a Drought Tolerant Soybean Cultivar from Brazilian Savanna

Proteome analysis of sesame leaves in response to waterlogging stress at vegetative and flowering stages

The iTRAQ-based chloroplast proteomic analysis of Triticum aestivum L. leaves subjected to drought stress and 5-aminolevulinic acid alleviation reveals several proteins involved in the protection of photosynthesis

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