Proteomic and Metabolomic Analysis of a Drought Tolerant Soybean Cultivar from Brazilian Savanna

doi:10.20900/cbgg20190022

Cited by 9 publications

(6 citation statements)

References 38 publications

(60 reference statements)

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“…Soybean seeds from conventional cultivar BR16, considered drought-sensitive (Oya et al 2004;Lima et al 2019) were treated with fungicide Vitavax® Thiram 200 SC (200 g.L −1 ) (ADAPAR) for health quality purposes and then allowed to germinate on Germitest® paper in the dark in germinating chamber, for 4 days, at 28 ± 1°C and 100% relative humidity (RH). Seedlings were transferred to 5 kg pots filled with substrate mixture 1:1 (fertilized soil and washed sand), one plant per pot.…”

Section: Biological Materials and Greenhouse Experimentsmentioning

confidence: 99%

Flower and pod genes involved in soybean sensitivity to drought

Molinari

Fuganti-Pagliarini

Marcolino-Gomes

et al. 2021

Journal of Plant Interactions

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In soybean, studies on drought-responses are conducted during vegetative season. Information related to genes triggered in response to water-deficit (WD) in flower and pod is lacking. We performed an RNASeq and an agro-physiological characterization at stages R2 and R4 of soybean cultivar BR16, under WD. Physiological results showed a decrease in gas exchange parameters. Agronomical results showed WD impaired yield. Global Gene Ontology analyses indicated that most of the Differential Expressed Genes (DEGs) were down-regulated in flowers but up-regulated in pods. qRT-PCR revealed that WD triggered hormone biosynthesis changes. Mechanisms such as a decrease in glyceollin levels and cell wall instability were observed. This data showed tissuespecific mechanisms correlated with phenotypic responses such as drought escape, early flowering hormone-induced, and loss in grain weight. We presented here a comprehensive expression profile of flowers and pods soybean genes, which could guide researchers in the development of plants more tolerant to drought.

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Section: Biological Materials and Greenhouse Experimentsmentioning

confidence: 99%

Flower and pod genes involved in soybean sensitivity to drought

Molinari

Fuganti-Pagliarini

Marcolino-Gomes

et al. 2021

Journal of Plant Interactions

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“…Aliquots of 50 mg of lyophilized extracts were solubilized in 1.5 ml of cold extraction solution (1 : 2.5 : 1 water, methanol, chloroform) containing 60 μl of ribitol (0.2 mg • ml -1 stock in water) as an internal standard. Metabolic extraction and derivatization were performed following Lima et al (2019).…”

Section: Metabolic Profiling By Gc/msmentioning

confidence: 99%

Journal of Plant Protection Research

Alves¹,

Assis²,

Pádua³

et al. 2020

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Root-knot nematodes, genus Meloidogyne, are among the most plant damaging pathogens worldwide. The action of natural products against plant pathogens has been investigated to assess their effectiveness in the control of diseases. Thus, the present study aimed to evaluate the phytochemistry potential of the Ficus species for the control of Meloidogyne javanica. In vitro inhibitory activity assays were performed with crude ethanolic extracts of leaves and branches from 10 Ficus species. Among these, Ficus carica extracts exhibited strong paralysis activity against second stage juveniles (J2) (EC 50 = 134.90 μg • ml -1 ), after 72 hours. In addition, high efficacy was observed in egg-hatching inhibition at different embryonic stages. Microscopy analysis revealed severe morphological alterations in the nematode tissues at the J2 stage, as well as immotility of juveniles released from eggs in the presence of F. carica extracts. The efficacy of the treatments for the other species was very low. These differences were supported by the variation in the compound classes, mainly for alkaloids and metabolite profiles by Gas Chromatography/Mass Spectrometry (GC/MS) when F. carica was compared with the other species. The results indicated that F. carica is a promising source for the isolation and identification of molecules capable of acting in the control of M. javanica.

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“…In current studies, transcriptomic, proteomic, and metabolomic approaches are mostly used to identify the response of model plants to drought stress ( Chmielewska et al., 2016 ; Michaletti et al., 2018 ; Lima et al., 2019 ; Du et al., 2020 ; Guo et al., 2020 ; Zhou et al., 2022 ), but there is a lack of integrated research on metabolites involved in drought tolerance of forest trees. Therefore, the aim of this study was to measure changes in metabolite levels in the roots and needles of loblolly pine seedlings under long-term drought stress, and to identify major metabolic pathways in loblolly pine seedlings related to drought stress.…”

Section: Introductionmentioning

confidence: 99%

Targeted and untargeted metabolomics reveals deep analysis of drought stress responses in needles and roots of Pinus taeda seedlings

2023

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Drought stress is one of major environmental stresses affecting plant growth and yield. Although Pinus taeda trees are planted in rainy southern China, local drought sometime occurs and can last several months, further affecting their growth and resin production. In this study, P. taeda seedlings were treated with long-term drought (42 d), and then targeted and untargeted metabolomics analysis were carried out to evaluate drought tolerance of P. taeda. Targeted metabolomics analysis showed that levels of some sugars, phytohormones, and amino acids significantly increased in the roots and needles of water-stressed (WS) P. taeda seedlings, compared with well-watered (WW) pine seedlings. These metabolites included sucrose in pine roots, the phytohormones abscisic acid and sacylic acid in pine needles, the phytohormone gibberellin (GA4) and the two amino acids, glycine and asparagine, in WS pine roots. Compared with WW pine seedlings, the neurotransmitter acetylcholine significantly increased in needles of WS pine seedlings, but significantly reduced in their roots. The neurotransmitters L-glutamine and hydroxytyramine significantly increased in roots and needles of WS pine seedlings, respectively, compared with WW pine seedlings, but the neurotransmitter noradrenaline significantly reduced in needles of WS pine seedlings. Levels of some unsaturated fatty acids significantly reduced in roots or needles of WS pine seedlings, compared with WW pine seedlings, such as linoleic acid, oleic acid, myristelaidic acid, myristoleic acid in WS pine roots, and palmitelaidic acid, erucic acid, and alpha-linolenic acid in WS pine needles. However, three saturated fatty acids significantly increased in WS pine seedlings, i.e., dodecanoic acid in WS pine needles, tricosanoic acid and heptadecanoic acid in WS pine roots. Untargeted metabolomics analysis showed that levels of some metabolites increased in WS pine seedlings, especially sugars, long-chain lipids, flavonoids, and terpenoids. A few of specific metabolites increased greatly, such as androsin, piceatanol, and panaxatriol in roots and needles of WS pine seedlings. Comparing with WW pine seedlings, it was found that the most enriched pathways in WS pine needles included flavone and flavonol biosynthesis, ABC transporters, diterpenoid biosynthesis, plant hormone signal transduction, and flavonoid biosynthesis; in WS pine roots, the most enriched pathways included tryptophan metabolism, caffeine metabolism, sesquiterpenoid and triterpenoid biosynthesis, plant hormone signal transduction, biosynthesis of phenylalanine, tyrosine, and tryptophan. Under long-term drought stress, P. taeda seedlings showed their own metabolomics characteristics, and some new metabolites and biosynthesis pathways were found, providing a guideline for breeding drought-tolerant cultivars of P. taeda.

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Proteomic and Metabolomic Analysis of a Drought Tolerant Soybean Cultivar from Brazilian Savanna

Cited by 9 publications

References 38 publications

Flower and pod genes involved in soybean sensitivity to drought

Flower and pod genes involved in soybean sensitivity to drought

Journal of Plant Protection Research

Targeted and untargeted metabolomics reveals deep analysis of drought stress responses in needles and roots of Pinus taeda seedlings

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