Chlorophyll Fluorescence as an Indicator of Photosynthetic Behaviour of In Vitro Chestnut during Acclimatization at High CO2 Availability

Carvalho, Lisiane Fernandes de; Osório, María Leonor; Amâncio, Sara

doi:10.1007/978-94-011-3953-3_812

Cited by 23 publications

(33 citation statements)

References 8 publications

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“…Similar results were reported after transfer of Nicotiana (Pospíšilová et al 1999), Calathea (Van Huylenbroeck et al 2000), and Castanea (Carvalho et al 2001), to ex vitro conditions. Usually, mature leaves of plants exposed to increased CO 2 show a decline in total chlorophyll content and chlorophyll a/b ratio (see Van Oosten and Besford 1994).…”

Section: Discussionsupporting

confidence: 92%

Effects of CO<sub>2</sub> concentration on acclimatization and physiological responses of two cultivars of carob tree

Osório¹,

Gonçalves²,

Osório³

et al. 2005

Biologia plant.

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This study reports survival and physiological responses of micropropagated Ceratonia siliqua L. cvs. Galhosa and Mulata plants during ex vitro acclimatization under ambient (AC; 330 µmol mol -1 ) or elevated (EC; 810 µmol mol -1 ) CO 2 concentration and a photosynthetic photon flux density of 125 µmol m -2 s -1 . CO 2 enrichment during acclimatization did not improve survival rate that was around 80 % for both treatments. Eight weeks after ex vitro transplantation, photosynthetic capacity and apparent quantum yield in acclimatized leaves were higher in comparison with those in in vitro-grown leaves, without any significant difference between CO 2 treatments. Chlorophyll content increased after acclimatization. However, EC led to a decrease in the total amount of chlorophyll in new leaves of both cultivars, compared to those grown at AC. Soluble sugars and starch contents were not markedly affected by growth EC, although starch had significantly increased after transfer to ex vitro conditions. EC induced an increase in the stem elongation and in the effective life of leaves, and a decrease in the number of new leaves.

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Section: Discussionsupporting

confidence: 92%

Effects of CO<sub>2</sub> concentration on acclimatization and physiological responses of two cultivars of carob tree

Osório¹,

Gonçalves²,

Osório³

et al. 2005

Biologia plant.

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“…Touriga Nacional (Carvalho et al, 2001). Segundo estes autores, a produção de biomassa total é o parâmetro mais confiável para avaliar o crescimento e a multiplicação in vitro.…”

Section: Resultsunclassified

“…Além da propagação, é possível avaliar e caracterizar genótipos tomando-se por base parâmetros morfofisiológicos do crescimento, como a utilização do CO 2 e produção de biomassa (Galzy & Compan, 1992;Silva et al, 1996;Fila et al, 1998;Moreira, 2000), teor de clorofila (Restagno et al, 1995;Amân-cio et al, 1999;Carvalho et al, 2001), características estomáticas (Silva & Doazan, 1995;Silva et al, 1996;Moreira, 2000), transpiração e potencial hídrico das folhas (Fila et al, 1998;Iacono & Martinelli, 1998) que são parâmetros fundamentais para a micropropagação e aclimatização de plantas.…”

Section: Introductionunclassified

Propagação in vitro e avaliação de parâmetros morfofisiológicos de porta-enxertos de videira

Borghezan

Moraes

Moreira

et al. 2003

Pesq. agropec. bras.

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Resumo -A micropropagação de genótipos selecionados pode contribuir para atender a demanda de plantas matrizes e mudas de qualidade genética e sanitária comprovadas de videira (Vitis spp.) no Estado de Santa Catarina. O objetivo deste trabalho foi estabelecer e multiplicar in vitro porta-enxertos de videira e avaliar parâmetros morfofisiológicos fundamentais à micropropagação e aclimatização. Os porta-enxertos VR043-43, VR039-16, Paulsen 1103, R110, SO4 e Kober 5BB foram estabelecidos e multiplicados in vitro pelo método de gemas axilares em meio de cultura DSD1. Quarenta e dois por cento dos explantes foram estabelecidos in vitro. Houve variabilidade de crescimento, área foliar e matéria seca entre os genótipos. O porta-enxerto Paulsen 1103 foi numericamente superior aos demais no desenvolvimento in vitro em comprimento de caule (6,2 cm), produção de biomassa (34,8 mg) e área foliar (18,1 cm 2 ) in vitro. O teor de clorofila total variou entre os porta-enxertos e o ambiente de cultura, com 0,7 e 2,8 mg/g de matéria fresca do R110 (in vitro) e VR043-43 (ex vitro), respectivamente. A maior (216,4/mm 2 ) e a menor (119,2/mm 2 ) densidade estomática foram apresentadas pelo VR039-16 in vitro e pelo SO4 ex vitro, respectivamente. A taxa de sobrevivência de plantas na aclimatização foi em média 90,3±1,1% por genótipo. Os porta-enxertos de videira avaliados apresentaram características morfofisiológicas apropriadas para a propagação in vitro e a transferência ex vitro.Termos para indexação: Vitis, micropropagação, biomassa, estômatos, clorofila. In vitro propagation and evaluation of morphophysiologic parameters of grapevine rootstocksAbstract -Micropropagation of selected genotypes can be valuable to achieve demand for elite plants with genetic fidelity and high sanitary quality of grapevine (Vitis spp.) in Santa Catarina State, Brazil. The objective of this work was to propagate grapevine rootstocks in vitro and to evaluate important morphophysiologic parameters for the plants micropropagation and acclimatization. Axillary buds of the rootstocks grapevine VR043-43, VR039-16, Paulsen 1103, R110, SO4 and Kober 5BB were inoculated in DSD1 culture medium. Rates of 42% of explants were established in vitro. There was variation in growth, leaf area and dry weight among the genotypes. Paulsen 1103 revealed superior features in vitro for length of the stem (6.2 cm), biomass production (34.8 mg) and leaf area (18.1 cm 2 ). The chlorophyll content showed variation among the rootstocks and the environment of the culture, ranging from 0.7 to 2.8 mg/g in fresh weight for R110 (in vitro) and VR043-43 (ex vitro), respectively. The highest (216.4/mm 2 ) and the lowest (119.2/mm 2 ) stomata number were shown by VR039-16 cultivated in vitro and by SO4 ex vitro, respectively. In the acclimatization stage, the mean of planting stock survival rate was 90.3±1.1% per genotype. The evaluated grapevine rootstocks present morphophysiologic parameters appropriated to in vitro propagation and ex vitro transference.

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“…Improving the photosynthetic functioning of in vitro plantlets that are transferred to ex vitro is essential to assure high survival rates after transfer to in vivo conditions (Carvalho et al 2001). Reduced leaf photosynthetic activity as a result of the poor development of the photosynthetic apparatus, inadequate gas exchange, stomata deformation and suppressed Rubisco activity has been revealed to limit successful in vitro to ex vitro transfer (Zenkteler and Borkowska 2002;Apóstolo et al 2005;Joshi et al 2006;de Oliveira et al 2008 and the literature therein).…”

Section: Discussionmentioning

confidence: 99%

Rooting affects the photosystem II activity: in vitro and ex vitro studies on energy hybrid sorrel

et al. 2017

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Rumex tianschanicus 9 Rumex patientia is a high-biomass-yielding plant suitable for fuel and biogas production. The protocol of the hybrid sorrel micropropagation was used to study the changes in the photosystem II (PSII) activity as well as to analyse the ultrastructure of the chloroplasts. The lowest effective PSII quantum yield [Y(II)] and an apparent electron transport rate of PSII [ETR(II)] were observed for adventitious shoots that had been regenerated in vitro, before rooting. These fluorescence parameters were higher and similar for both the leaves of the same adventitious shoots that had been rooted under in vitro conditions and for the shoots that had been acclimated and grown in ex vitro conditions. The analysis indicated that the PSII activity strongly depends on the formation of properly functioning roots and that in vitro or ex vitro culture conditions are, at least to some degree, less important. TEM analysis revealed that chloroplasts from plants rooted in vitro were sufficiently mature and acclimatization processes have less impact on their development. This is the first report concerning the analysis of PSII activity and the ultrastructure of the chloroplasts at all of the stages of micropropagation, i.e. adventitious shoot formation in vitro, rooting in vitro and acclimation to ex vitro conditions. It strongly indicated that rooting under in vitro conditions, rather than the acclimation to ex vitro conditions, plays a key role in the development of a completely functional photosynthetic apparatus in hybrid sorrel.Keywords Rumex tianschanicus 9 Rumex patientia Á PSII activity Á Chlorophyll a fluorescence Á Ultrastructure of chloroplasts Á In vitro/ex vitro transfer Á Photosynthetic apparatus

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Chlorophyll Fluorescence as an Indicator of Photosynthetic Behaviour of In Vitro Chestnut during Acclimatization at High CO2 Availability

Cited by 23 publications

References 8 publications

Effects of CO<sub>2</sub> concentration on acclimatization and physiological responses of two cultivars of carob tree

Effects of CO<sub>2</sub> concentration on acclimatization and physiological responses of two cultivars of carob tree

Propagação in vitro e avaliação de parâmetros morfofisiológicos de porta-enxertos de videira

Rooting affects the photosystem II activity: in vitro and ex vitro studies on energy hybrid sorrel

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