Chitosan stabilized camptothecin nanoemulsions: Development, evaluation and biodistribution in preclinical breast cancer animal mode

Natesan, Subramanian; Sugumaran, Abimanyu; Ponnusamy, Chandrasekar; Thiagarajan, Vignesh; Palanichamy, Rajaguru; Kandasamy, Ruckmani

doi:10.1016/j.ijbiomac.2017.05.127

Cited by 49 publications

(23 citation statements)

References 25 publications

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“…Regarding CNNEs, the dynamic layer of chitosan creates additional partition through continuous phase. This leads to slow release of drug from the oil core and it could be predicted that it will maintain the therapeutic window at the site of action for a longer period of time [ 53 ]. Therefore, it is expected to obtain a prolonged release of incorporated NAR at the site of application to maintain the supply of the therapeutic agent towards effective healing of wound.…”

Section: Resultsmentioning

confidence: 99%

Development and Optimization of Naringenin-Loaded Chitosan-Coated Nanoemulsion for Topical Therapy in Wound Healing

et al. 2020

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The potential role of naringenin (NAR), a natural flavonoid, in the treatment of chronic wound has prompted the present research to deliver the drug in nanoemulsion (NE) form, where synergistic role of chitosan was achieved through development of chitosan-coated NAR NE (CNNE). The NE consisted of Capryol 90, Tween 20 and Transcutol P, which was fabricated by low-energy emulsification method to encapsulate NAR within the oil core. The optimization of the formulated NEs was performed using Box–Behnken statistical design to obtain crucial variable parameters that influence globule size, size distribution and surface charge. Finally, the optimized formulation was coated with different concentrations of chitosan and subsequently characterized in vitro. The size of the CNNE was found to be increased when the drug-loaded formulation was coated with chitosan. Controlled release characteristics depicted 67–81% release of NAR from the CNNE, compared to 89% from the NE formulation. Cytotoxicity study of the formulation was performed in vitro using fibroblast cell line (NIH-3T3), where no inhibition in proliferation of the cells was observed with CNNE. Finally, the wound healing potential of the CNNE was evaluated in an abrasion-created wound model in experimental animals where the animals were treated and compared histologically at 0 and 14 days. Significant improvement in construction of the abrasion wound was observed when the animals were treated with formulated CNNE, whereas stimulation of skin regeneration was depicted in the histological examination. Therefore, it could be summarized that the chitosan coating of the developed NAR NE is a potential platform to accelerate healing of wounds.

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Section: Resultsmentioning

confidence: 99%

Development and Optimization of Naringenin-Loaded Chitosan-Coated Nanoemulsion for Topical Therapy in Wound Healing

et al. 2020

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“…Other authors encapsulated cholesterol derivatives, such as 7-ketocholesterol, into lipid core nanoemulsions and assess them in vivo in a murine melanoma cell line where it was demonstrated that the nanoemulsion decreases the tumor size more than 50%, enlarged the necrotic area, and reduced intratumoral vasculature. The in vitro uptake into tumor cells was LDL-receptor-mediated cell internalization and demonstrated that a single dose of the cholesterol nanoemulsions killed 10% of melanoma cells [106].…”

Section: Nanoemulsions Applied To Cancer Therapymentioning

confidence: 99%

Current Applications of Nanoemulsions in Cancer Therapeutics

et al. 2019

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Nanoemulsions are pharmaceutical formulations composed of particles within a nanometer range. They possess the capacity to encapsulate drugs that are poorly water soluble due to their hydrophobic core nature. Additionally, they are also composed of safe gradient excipients, which makes them a stable and safe option to deliver drugs. Cancer therapy has been an issue for several decades. Drugs developed to treat this disease are not always successful or end up failing, mainly due to low solubility, multidrug resistance (MDR), and unspecific toxicity. Nanoemulsions might be the solution to achieve efficient and safe tumor treatment. These formulations not only solve water-solubility problems but also provide specific targeting to cancer cells and might even be designed to overcome MDR. Nanoemulsions can be modified using ligands of different natures to target components present in tumor cells surface or to escape MDR mechanisms. Multifunctional nanoemulsions are being studied by a wide variety of researchers in different research areas mainly for the treatment of different types of cancer. All of these studies demonstrate that nanoemulsions are efficiently taken by the tumoral cells, reduce tumor growth, eliminate toxicity to healthy cells, and decrease migration of cancer cells to other organs.

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“…21,22 HA has several advantages such as high biocompatibility, biodegradability, nonimmunogenicity, noninflammatory, nontoxicity, and low nonspecific interactions with serum components. 6,23,24 Particularly, HA has demonstrated specific tumor-targeting capability due to the strong affinity for CD44 receptors, a cell surface glycoprotein, which are overexpressed in a majority of cancer cells rather than normal cells. 25−28 Therefore, HA is able to open a novel door for active targeted delivery of CPT against CD44 overexpressed cancer cells through receptor-mediated endocytosis pathway.…”

Section: Introductionmentioning

confidence: 99%

Hyaluronic Acid-Coated Camptothecin Nanocrystals for Targeted Drug Delivery to Enhance Anticancer Efficacy

Wang

Muhammad

et al. 2020

Mol. Pharmaceutics

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Tumor-targeted drug delivery via chemotherapy is very effective on cancer treatment. For potential anticancer agent such as Camptothecin (CPT), high chemotherapeutic efficacy and accurate tumor targeting are equally crucial. Inspired by special CD44 binding capability from hyaluronic acid (HA), in this study, novel HA-coated CPT nanocrystals were successfully prepared by an antisolvent precipitation method for tumor-targeted delivery of hydrophobic drug CPT. These HA-coated CPT nanocrystals demonstrated high drug loading efficiency, improved aqueous dispersion, prolonged circulation, and enhanced stability resulting from their nanoscaled sizes and hydrophilic HA layer. Moreover, as compared to crude CPT and naked CPT nanocrystals, HA-coated CPT nanocrystals displayed dramatically enhanced in vitro anticancer activity, apoptosis-inducing potency against CD44 overexpressed cancer cells, and lower toxic effect toward normal cells due to pH-responsive drug release behavior and specific HA-CD44 mediated endocytosis. Additionally, HA-coated CPT nanocrystals performed fairly better antimigration activity and biocompatibility. The possible molecular mechanism regarding this novel drug formulation might be linked to intrinsic mitochondria-mediated apoptosis by an increase of Bax to Bcl-2 ratio and upregulation of P53. Consequently, HA-coated CPT nanocrystals are expected to be an effective nanoplatform in drug delivery for cancer therapy.

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Chitosan stabilized camptothecin nanoemulsions: Development, evaluation and biodistribution in preclinical breast cancer animal mode

Cited by 49 publications

References 25 publications

Development and Optimization of Naringenin-Loaded Chitosan-Coated Nanoemulsion for Topical Therapy in Wound Healing

Development and Optimization of Naringenin-Loaded Chitosan-Coated Nanoemulsion for Topical Therapy in Wound Healing

Current Applications of Nanoemulsions in Cancer Therapeutics

Hyaluronic Acid-Coated Camptothecin Nanocrystals for Targeted Drug Delivery to Enhance Anticancer Efficacy

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