Chitosan nanoparticles as a biocompatible and efficient nanowagon for benzyl isothiocyanate

Uppal, Shivani; Kaur, Khushwinder; Kumar, Rajendra; Kaur, Naval Deep; Shukla, Geeta; Mehta, S.K.

doi:10.1016/j.ijbiomac.2018.04.036

Cited by 33 publications

(26 citation statements)

References 38 publications

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“…CNP has been reported as a good biocompatible nanocarrier system [ 81 , 82 ]. In order to evaluate whether its hydrophobically-modified complement, pCNP is biocompatible as well, MTT cytotoxicity assay was performed against the A549 lung cancer cells.…”

Section: Resultsmentioning

confidence: 99%

Increased Cytotoxic Efficacy of Protocatechuic Acid in A549 Human Lung Cancer Delivered via Hydrophobically Modified-Chitosan Nanoparticles As an Anticancer Modality

et al. 2020

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The growing incidence of global lung cancer cases against successful treatment modalities has increased the demand for the development of innovative strategies to complement conventional chemotherapy, radiation, and surgery. The substitution of chemotherapeutics by naturally occurring phenolic compounds has been touted as a promising research endeavor, as they sideline the side effects of current chemotherapy drugs. However, the therapeutic efficacy of these compounds is conventionally lower than that of chemotherapeutic agents due to their lower solubility and consequently poor intracellular uptake. Therefore, we report herein a hydrophobically modified chitosan nanoparticle (pCNP) system for the encapsulation of protocatechuic acid (PCA), a naturally occurring but poorly soluble phenolic compound, for increased efficacy and improved intracellular uptake in A549 lung cancer cells. The pCNP system was modified by the inclusion of a palmitoyl group and physico-chemically characterized to assess its particle size, Polydispersity Index (PDI) value, amine group quantification, functional group profiling, and morphological properties. The inclusion of hydrophobic palmitoyl in pCNP-PCA was found to increase the encapsulation of PCA by 54.5% compared to unmodified CNP-PCA samples whilst it only conferred a 23.4% larger particle size. The single-spherical like particles with uniformed dispersity pCNP-PCA exhibited IR bands, suggesting the successful incorporation of PCA within its core, and a hydrophobic layer was elucidated via electron micrographs. The cytotoxic efficacy was then assessed by using an MTT cytotoxicity assay towards A549 human lung cancer cell line and was compared with traditional chitosan nanoparticle system. Fascinatingly, a controlled release delivery and enhanced therapeutic efficacy were observed in pCNP-PCA compared to CNP, which is ascribed to lower IC50 values in the 72-h treatment in the pCNP system. Using the hydrophobic system, efficacy of PCA was significantly increased in 24-, 48-, and 72-h treatments compared to a single administration of the compound, and via the unmodified CNP system. Findings arising from this study exhibit the potential of using such modified nanoparticulate systems in increasing the efficacy of natural phenolic compounds by augmenting their delivery potential for better anti-cancer responses.

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Section: Resultsmentioning

confidence: 99%

Increased Cytotoxic Efficacy of Protocatechuic Acid in A549 Human Lung Cancer Delivered via Hydrophobically Modified-Chitosan Nanoparticles As an Anticancer Modality

et al. 2020

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“…In addition to these attributes, as an example, benzyl ITC, has shown bacteriostatic, bacteriocidal and anthelmintic and vermifuge properties at low concentrations. Uppal et al [101] discuss this but raised the caveat of poor bioavailability for a number of intrinsic chemical properties [68] . Uppal et al [101] then showed that within a complex of chitosan nanoparticles as carriers there is significantly improved efficacy.…”

Section: Therapeutic Strategymentioning

confidence: 99%

“…Uppal et al [101] discuss this but raised the caveat of poor bioavailability for a number of intrinsic chemical properties [68] . Uppal et al [101] then showed that within a complex of chitosan nanoparticles as carriers there is significantly improved efficacy. In fact, bio-nanocarriers have proven highly advantageous for efficient delivery of other promising phytochemical therapeutics such as curcumin [102] .…”

Section: Therapeutic Strategymentioning

confidence: 99%

Perspective on dietary isothiocyanates in the prevention, development and treatment of cancer

Yeger¹,

Mokhtari²

2020

JCMT

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Epidemiological evidence has highlighted the association of specific diets and a lower incidence of cancer. Foremost, the Mediterranean diet provides high levels of polyphenolics and a high consumption of healthier fats, e.g., as from olive oil. In the Mediterranean region the consumption of vegetables is elevated providing a class of compounds, the isothiocyanates (ITCs) as found in the cabbage family. The ITCs have raised great interest for their health benefits over the past few decades. Some of the key ITC compounds, sulforaphane, phenethylisothiocyanate and benzyl isothiocyanate, have been studied in vitro and in vivo and the data support their promise for cancer chemoprevention, as anti-tumor agents, and for chemoprotection of normal tissues and organs. Along with other polyphenolic compounds in the diet, in general, they also possess key anti-inflammatory properties thus satisfying the criteria for compounds that could intervene in cancer initiation and progression. In this review we provide a larger overview of the advantages of including ITCs in the diet as food or as supplements and speculate on what could constitute a valuable therapeutic strategy for improving and sustaining good health and countering cancer disease in humans.

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“…(2011)Benzyl isothiocyanateInclusion complexβ-cyclodextrinFreeze-drying86.4 ± 2.6Li et al. (2015a)Benzyl isothiocyanateEmulsion ionic gelationChitosan nanoparticlesFreeze-drying64.7 ± 4.7Uppal et al. (2018)∗Standard deviation not reported.…”

Section: Microencapsulation Of Sulforaphane and Isothiocyanatesmentioning

confidence: 99%

“…The nano emulsion showed superior protection against AITC degradation (78% remaining after 60 d at 30 °C), compared with protein nanoparticles as well as a non-encapsulated aqueous dispersion. Uppal et al. (2018) encapsulated benzyl isothiocyanate through emulsion ionic gelation using chitosan, and obtained an encapsulation efficiency of 64.7%.…”

Section: Microencapsulation Of Sulforaphane and Isothiocyanatesmentioning

confidence: 99%

Insights about stabilization of sulforaphane through microencapsulation

Zambrano

Bustos

Mahn

2019

Heliyon

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The health–promoting properties of sulforaphane (SFN) are well known, however its instability is still a hurdle for its incorporation into food matrices. SFN can be stabilized by microencapsulation, technique sparingly explored for isothiocyanates so far. This review summarizes the advances in microencapsulation of SFN and other isothiocyanates. Encapsulation efficiency and degradation rate of sulforaphane in different systems are compared and discussed. Ionic gelation and complex coacervation seem more adequate for SFN, both underexplored until now. Drying conditions after chemical encapsulation are determinant, most likely related to thermal degradation of SFN. The current information is insufficient to identify the most adequate encapsulation system and the optimal process conditions to stabilize SFN aiming at its incorporation into food matrices. Accordingly, encapsulation conditions should be investigated, which arises as a new research line. Stability studies are encouraged since this information will help in designing SFN microencapsulation strategies that extend the industrial application of this promising health-promoting compound.

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Chitosan nanoparticles as a biocompatible and efficient nanowagon for benzyl isothiocyanate

Cited by 33 publications

References 38 publications

Increased Cytotoxic Efficacy of Protocatechuic Acid in A549 Human Lung Cancer Delivered via Hydrophobically Modified-Chitosan Nanoparticles As an Anticancer Modality

Increased Cytotoxic Efficacy of Protocatechuic Acid in A549 Human Lung Cancer Delivered via Hydrophobically Modified-Chitosan Nanoparticles As an Anticancer Modality

Perspective on dietary isothiocyanates in the prevention, development and treatment of cancer

Insights about stabilization of sulforaphane through microencapsulation

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