Chitosan film containing fucoidan as a wound dressing for dermal burn healing: Preparation and in vitro/in vivo evaluation

Abstract

The aim of this study was to develop chitosan film containing fucoidan and to investigate its suitability for the treatment of dermal burns on rabbits. Porous films, thickness between 29.7 and 269.0 μm, were obtained by the solvent dropping method. Water vapor permeability (3.3-16.6/0.1 g), the swelling (0.67-1.77 g/g), tensile strength (7.1-45.8 N), and bioadhesion (0.076-1.771 mJ/cm 2 ) of the films were determined. The thinnest films were obtained with the lowest chitosan concentration (P G .05). The water absorption capacity of the films sharply increased with the freeze-drying technique. The film having the thickness of 29.7 μm showed the highest amount of moisture permeability (16.6 g/0.1 g). Higher chitosan concentration significantly increased tensile strength of the films (P G .05). Using higher concentration of lactic acid made films more elastic and applicable, and these films were selected for in vivo studies. Seven adult male New Zealand white rabbits were used for the evaluation of the films on superficial dermal burns. Biopsy samples were taken at 7, 14, and 21 days after wounding, and each wound site was examined macroscopically and histopathologically. After 7 days treatment, fibroplasia and scar were observed on wounds treated with fucoidan-chitosan film. The best regenerated dermal papillary formation, best reepithelization, and the fastest closure of wounds were found in the fucoidan-chitosan film treatment group after 14 days compared with other treatment and control groups. It can be concluded that fucoidan-chitosan films might be a potential treatment system for dermal burns and that changing formulation variables can modulate the characterizations of the films.

Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits

“…These projections help to anchor the epidermis to the dermis. 44,45) Our results demonstrated the number of rete pegs in the 1300Ϯ109 1333Ϯ93 1455Ϯ64 111Ϯ10 96Ϯ2 162Ϯ10 121Ϯ8 14 1950Ϯ82 1941Ϯ107 2358Ϯ40 2086Ϯ134 154Ϯ5 136Ϯ3 261Ϯ16 187Ϯ9 21 3533Ϯ196 3666Ϯ152 5566Ϯ555 3586Ϯ149 134Ϯ8 162Ϯ21 189Ϯ23 146Ϯ10 group treated with fucoidan-chitosan hydrogel was higher than the other groups during treatment period (pϽ0.05) and reached highest level (8.3Ϯ2.8) on day 21 (Table 4, Fig. 6).…”

“…The highest number of the nuclear organized regions (NOR) in the epithelial cell nucleus, stating the division and proliferation of cells, were seen in the fucoidan-chitosan group on day 14 (8.5Ϯ0.1) when compared to other groups (pϽ0.05) ( Table 4) and this finding was consistent with the results of previous studies. 44,45) As a result, it was proved that the fucoidan-chitosan hydrogel was found highly effective to increase the healing and re-epithelization of the wound by day 21.…”

Pythiosis cutánea en equinos tratados con acetonida de triamcinolona. Parte 3. Análisis histomorfométrico

“…Eso explica la correlación significativa entre los fibroblastos y las fibras de colágeno. El colágeno es un componente fundamental en el proceso de reparo tecidual, sintetizado a partir de los fibroblastos y presente en abundancia en la fase proliferativa y de remodelación de la herida (28). Los tipos de colágenos más expresivos en el proceso de cicatrización son los tipos I y III.…”

“…This explains the significant correlation between fibroblasts and collagen fibers. Collagen is a fundamental component in the tissue repair process, it is synthesized from fibroblasts and it is found abumdantly during the proliferative and wound healing phases (28). Collagen types I and III were the most expressive collagen types found during the healing process.…”

Canine Extremity Wound Treatment with Chitosan Extracted from Shrimp Shells: A Case Report

“…The findings were overlapped by Balazzi et al [1], Azuma et al [4], Usami et al al. [12], Da silva et al [14], Alemdaroglu et al [18] and Sezer et al [19]. Post traumatic hemorrhage remains one of the most mortal causes of early death with progressive seconder infections in wounds.…”