Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits

Abstract

Burn healing includes a specific biological process related to the general phenomenon of growth and regeneration and the primary objective in burn care is the promotion of rapid wound healing with the best functional results. The principal function of a burn dressing is to provide an optimum healing milieu for natural healing and desirable burn dressing may, therefore, be characterized on the basis of its performance such as; (a) provision of adequate gaseous exchange, (b) provision and maintenance of a moist environment, (c) protection from infections and contamination, (d) absorption of wound fluids and (e) painless and easy removal. 1,2) Hydrogels are ideal biopolymeric pharmaceutical forms for the treatment of skin wounds. They have low interfacial tension, high molecular and oxygen permeability, good moisturizing and mechanical properties that resemble physiological soft tissue. 3,4) For this reasons, polysaccharides, e.g. chitosan, are having hydrogel forming properties have been considered to be advantageous in its application as a wound dressing material. 5) Chitosan, [a (1→4) 2-amino-2-deoxy-b-D-glucan], a unique polysaccharide derived from deacetylation of chitin, has been used in wound treatment owing to its good biocompatibility, biodegradability and accelerated granulation. [6][7][8] On the other hand, fucoidan is a sulphated polyfucose polysaccharide and has attracted considerable biotechnological research interest since the discovery that it possessed anti-coagulant activity similar to that of heparin and also reported to possess other properties including antithrombotic, anti-inflammatory, anti-tumoral and anti-viral effects. 9,10) Many of these effects are thought to be due to its interaction with growth factors such as basic fibroblast growth factor (bFGF) and transforming growth factor-b (TGF-b). Fucoidan may, therefore, be able to modulate growth factordependent pathways in the cell biology of tissue repair. 11) Although a great number of studies on different pharmacological properties of fucoidan and chitosan are present, there is little information on the fucoidan-based system used in burn healing and its only limited with cell culture. 12) The aim of this study was to prepare fucoidan-chitosan hydrogel and to investigate its treatment efficiency on dermal burns on rabbits. MATERIALS AND METHODSMaterials Chitosan (MW 250 kDa, deacetylation degree Ն90%, Pronova A/S, Norway; MW 400 kDa, deacetylation degree Ն60%, Fluka, Germany; MW 750 kDa, deacetylation degree Ն75%, Sigma, U.S.A.), Fucoidan (from Fucus vesiculosus) and lactic acid (85% m/v) purchased from Sigma, U.S.A. All other reagents used were of analytical grade.Preparation of Hydrogels The composition of gel formulations was given in Table 1. Fucoidan was dissolved in 1% m/v lactic acid solution by mechanical shaking at 300 rpm for 1 h. Then chitosan was left to swell in the fucoidan solution overnight to prepare hydrogels. 13) Formulations were kept at 4°C until experiment.Viscosity Measurement The viscosity of hydrogels was determine...

The Effect of β-Glycerophosphate Crosslinking on Chitosan Cytotoxicity and Properties of Hydrogels for Vaginal Application

“…Hardness describes the ability to remove the formulation from the container. Low value of hardness is related to the ease of product removal and application but on the contrary, may decrease the retention time at the administration site [40]. In addition, together with the compressibility factor, hardness reflects the applicability to the desired site [41].…”

Can Fucoidan Decrease the Mortalities Caused by Columnaris Disease in Nile Tilapia?

“…Therefore, this study demonstrated apparent anti-bacterial and cutaneous wound healing effects of fucoidan. Fucoidan is known for its anti-bacterial, anti-viral, immunostimulatory, and healing stimulating effects [2][3][4][5][6][7][8][9][10][11]. In a previous study, fucoidan inhibited the growth of Staphylococcus aureus and Escherichia coli [5].…”

“…Fucoidan was also used as a prevention and treatment measure to control diseases caused by Helicobacter pylori [4]. Similutaneously, previous liteature demonstrated the cutaneous wound healing effect of fucoidan [10,11].…”

“…tangleweed (Lami-naria japonica), wakame (Undaria pinnati da Sporophyll) and limu moui (Cladosiphon okamuranus) [1]. Fucoidan is known for its anti-bacterial, anti-viral, immunostimulatory, and healing stimulating effects [2][3][4][5][6][7][8][9][10][11].…”

Fucoidan-Mimetic Glycopolymers : Synthesis and Biomedical Applications

“…There have been some works published where fucoidan has been used for drug delivery [2,[7][8][9][10][11][12][13], stem cell growth [2], wound healing [2,7,8,14], bone regeneration [2,[15][16][17][18], and in anti-bacterial [19] and cell culture hydrogels [20]. However, compared to the number of reports on the structure and bioactivities of fucoidan, the use of fucoidan in biomedical applications other than as a conventional drug is far less common.…”