Chitosan–DNA complexes: Charge inversion and DNA condensation

Amaduzzi, Francesca; Bomboi, Francesca; Bonincontro, A.; Bordi, F.; Casciardi, Stefano; Chronopoulou, Laura; Diociaiuti, Marco; Mura, Francesco; Palocci, Cleofe; Sennato, Simona

doi:10.1016/j.colsurfb.2013.09.029

Cited by 50 publications

(42 citation statements)

References 59 publications

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“…Simple complexation between the chitosan polymer and siRNA or DNA involves electrostatic interactions between cationic chitosan and anionic nucleic acids [34,35,36]. At proper N/P ratios, chitosan forms complexes in micro- or nano-sized particles.…”

Section: Chitosan As Gene Delivery Vehicle For Cancer Therapymentioning

confidence: 99%

Multifaceted Applications of Chitosan in Cancer Drug Delivery and Therapy

2017

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Chitosan is a versatile polysaccharide of biological origin. Due to the biocompatible and biodegradable nature of chitosan, it is intensively utilized in biomedical applications in scaffold engineering as an absorption enhancer, and for bioactive and controlled drug release. In cancer therapy, chitosan has multifaceted applications, such as assisting in gene delivery and chemotherapeutic delivery, and as an immunoadjuvant for vaccines. The present review highlights the recent applications of chitosan and chitosan derivatives in cancer therapy.

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Section: Chitosan As Gene Delivery Vehicle For Cancer Therapymentioning

confidence: 99%

Multifaceted Applications of Chitosan in Cancer Drug Delivery and Therapy

2017

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“…The preparation was stirred for 10 min and then stored at 4°C. Here, the ratio between the chitosan aminic groups and the DNA phosphate groups (i.e., the N/P ratio) was ten, as previously reported (Amaduzzi et al 2014).…”

Section: Chitosan Depolymerization and Characterizationmentioning

confidence: 92%

Improved stability and efficacy of chitosan/pDNA complexes for gene delivery

et al. 2014

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Among polymeric polycations, chitosan has emerged as a powerful carrier for gene delivery. Only a few studies have focused on the stability of the chitosan/DNA complex under storage, although this is imperative for nanomedicinal applications. Here, we synthesized polyelectrolyte complexes at a charge ratio of 10 using 50 kDa chitosan and plasmid (p)DNA that encodes a GFP reporter. These preparations were stable up to 3 months at 4 °C and showed reproducible transfection efficiencies in vitro in HEK293 cells. In addition, we developed a methodology that increases the in vitro transfection efficiency of chitosan/pDNA complexes by 150% with respect to standard procedures. Notably, intracellular pDNA release and transfected cells peaked 5 days following transection of mitotically active cells. These new developments in formulation technology enhance the potential for polymeric nanoparticle-mediated gene therapy.

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“…Tapping mode AFM topographs of ELP(-NH 2 ) complexed with pUC19 plasmid (Figures 6a to f) showed that the structures of the obtained polyplexes depend on the selected charge ratio (N+S)/P and yield mainly globular shapes [84,91,101]. Figure 6a shows the AFM topography (Figure 7).…”

Section: Detailed Structure Of Puc19 Plasmid Dna/elp(-nh 2 ) Complexementioning

confidence: 99%

“…From Figure 4b, it is possible to observe the particle size dependence of pUC19 plasmid/ELP(-NH 2 ) complexes as a function (N+S)/P ratio. It is worth mentioning that each solution of complexes was prepared at a chosen charge ratio, avoiding passing through the isoelectric point of the particles (ζ-potential=0 mV), where aggregates tend to form [91,92]. Rapid one-shot mixing (cationic ELPs to anionic DNA) method was the selected for the preparation of pUC19 plasmid/ELP(-NH 2 ) polyelectrolyte complexes.…”

Section: Charge Ratio Influence On Net Charge Dimensions and Morpholmentioning

confidence: 99%

Nucleic acids complexation with cationic elastin-like polypeptides: Stoichiometry and stability of nano-assemblies

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Garbay

Nando-Rodríguez

et al. 2019

Journal of Colloid and Interface Science

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Positively charged elastin-like polypeptides (ELPs) were synthesized for the compaction of genetic material. A recombinant ELP (VPGXG) 40 with X=V,M (3:1) was post-modified in two steps to introduce chemoselectively either primary or secondary amine pendant groups at each methionine residue. Positively charged ELPs were characterized by SDS-PAGE, size exclusion chromatography, 1 H NMR, potentiometric titrations and dynamic light scattering to assess their purity and determine their degree of functionalization, molecular weight, isoelectric point and thermo-responsive behaviour. Electrostatic complexation between the different ELP derivatives and nucleic acids was studied to determine the stoichiometry of ELP S /nucleic acids complex formation, and to find optimal conditions leading to stable nanoparticles with controlled size and surface potential. The stability of these complexes was investigated in the presence of salts at physiological concentrations and in the presence of surfactant. This study revealed that two regimes of stable nanoparticles in terms of size and charge can be obtained from the electrostatic complexation between the primary amine containing ELP derivative, ELP(-NH 2 ), and plasmid DNA. Resulting complexes were found to be stable to dissociation for charge ratios up to 2.5 under physiological salt concentrations (154 mM NaCl), showing that plasmid DNA was completely condensed by the polycationic ELP and protected against electrolyte-mediated dissociation.

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Chitosan–DNA complexes: Charge inversion and DNA condensation

Cited by 50 publications

References 59 publications

Multifaceted Applications of Chitosan in Cancer Drug Delivery and Therapy

Multifaceted Applications of Chitosan in Cancer Drug Delivery and Therapy

Improved stability and efficacy of chitosan/pDNA complexes for gene delivery

Nucleic acids complexation with cationic elastin-like polypeptides: Stoichiometry and stability of nano-assemblies

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