Improved stability and efficacy of chitosan/pDNA complexes for gene delivery

Cifani, Noemi; Chronopoulou, Laura; Pompili, Barbara; Martino, Antonio Di; Bordi, F.; Sennato, Simona; Domenico, Enea Gino Di; Palocci, Cleofe; Ascenzioni, Fiorentina

doi:10.1007/s10529-014-1727-7

Cited by 21 publications

(10 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The main interactions between PEC include strong but reversible electrostatic and dipole-dipole association, as well as hydrogen and hydrophobic bonds [3]. Recently, numerous PEC have found application as carriers for drug delivery, enzyme immobilization, DNA binding, tissue engineering, biosensors, etc [4][5][6][7][8]. In all these cases it is important to notice that the stability of PEC could be affected by many factors including density of charges, degree of ionization, pH of reaction medium, concentration of polyelectrolytes, distribution of ionic groups, molecular weight, mixing ratio, order of reacting polyelectrolytes, and drying process [9].…”

Section: Introductionmentioning

confidence: 99%

Ultrasonic compatibilization of polyelectrolyte complex based on polysaccharides for biomedical applications

Belluzo

Medina

Cortizo

et al. 2016

Ultrasonics Sonochemistry

View full text Add to dashboard Cite

a b s t r a c tIn recent years, there has been an increasing interest in the design of biomaterials for cartilage tissue engineering. This type of materials must meet several requirements. In this study, we apply ultrasound to prepare a compatibilized blend of polyelectrolyte complexes (PEC) based on carboxymethyl cellulose (CMC) and chitosan (CHI), in order to improve stability and mechanical properties through the interpolymer macroradicals coupling produced by sonochemical reaction. We study the kinetic of the sonochemical degradation of each component in order to optimize the experimental conditions for PEC compatibilization. Scaffolds obtained applying this methodology and scaffolds without ultrasound processing were prepared and their morphology (by scanning electron microscopy), polyelectrolyte interactions (by FTIR), stability and mechanical properties were analyzed. The swelling kinetics was studied and interpreted based on the structural differences between the two kinds of scaffolds. In addition we evaluate the possible in vitro cytotoxicity of the scaffolds using macrophage cells in culture. Our results demonstrate that the ultrasound is a very efficient methodology to compatibilize PEC, exhibiting improved properties compared with the simple mixture of the two polysaccharides. The test with murine macrophage RAW 264.7 cells showed no evince of cytotoxicity, suggesting that PEC biomaterials obtained under ultrasound conditions could be useful in the cartilage tissue engineering field.

show abstract

Section: Introductionmentioning

confidence: 99%

Ultrasonic compatibilization of polyelectrolyte complex based on polysaccharides for biomedical applications

Belluzo

Medina

Cortizo

et al. 2016

Ultrasonics Sonochemistry

View full text Add to dashboard Cite

show abstract

“…This effect is known as substrate-mediated gene delivery [57,60]. In addition, it has been reported that stability of CS/pDNA or CS/RNA complexes would not change in storage under 4 °C [61,62]. Here, the CS-antimiR-138/HA PEM-functionalized microporous Ti surface show consistent transfection efficiency over The bone volume per total volume (BV/TV), the mean trabecular thickness (Tb.Th), the mean trabecular number (Tb.N) and the mean trabecular separation (Tb.Sp) within the ROI zone.…”

Section: Discussionmentioning

confidence: 68%

Chitosan-miRNA functionalized microporous titanium oxide surfaces via a layer-by-layer approach with a sustained release profile for enhanced osteogenic activity

Liu

et al. 2020

J Nanobiotechnol

View full text Add to dashboard Cite

Background The biofunctionalization of titanium implants for high osteogenic ability is a promising approach for the development of advanced implants to promote osseointegration, especially in compromised bone conditions. In this study, polyelectrolyte multilayers (PEMs) were fabricated using the layer-by-layer approach with a chitosan-miRNA (CS-miRNA) complex and sodium hyaluronate (HA) as the positively and negatively charged polyelectrolytes on microarc-oxidized (MAO) Ti surfaces via silane-glutaraldehyde coupling. Methods Dynamic contact angle and scanning electron microscopy measurements were conducted to monitor the layer accumulation. RiboGreen was used to quantify the miRNA loading and release profile in phosphate-buffered saline. The in vitro transfection efficiency and the cytotoxicity were investigated after seeding mesenchymal stem cells (MSCs) on the CS-antimiR-138/HA PEM-functionalized microporous Ti surface. The in vitro osteogenic differentiation of the MSCs and the in vivo osseointegration were also evaluated. Results The surface wettability alternately changed during the formation of PEMs. The CS-miRNA nanoparticles were distributed evenly across the MAO surface. The miRNA loading increased with increasing bilayer number. More importantly, a sustained miRNA release was obtained over a timeframe of approximately 2 weeks. In vitro transfection revealed that the CS-antimiR-138 nanoparticles were taken up efficiently by the cells and caused significant knockdown of miR-138 without showing significant cytotoxicity. The CS-antimiR-138/HA PEM surface enhanced the osteogenic differentiation of MSCs in terms of enhanced alkaline phosphatase, collagen production and extracellular matrix mineralization. Substantially enhanced in vivo osseointegration was observed in the rat model. Conclusions The findings demonstrated that the novel CS-antimiR-138/HA PEM-functionalized microporous Ti implant exhibited sustained release of CS-antimiR-138, and notably enhanced the in vitro osteogenic differentiation of MSCs and in vivo osseointegration. This novel miRNA-functionalized Ti implant may be used in the clinical setting to allow for more effective and robust osseointegration.

show abstract

“…Two plasmids (plasmid green fluorescent protein-pGFP and plasmid βgalactosidasepβgal) were used as models for transfection. To achieve the best transfection efficiency, we complexed DNA with chitosan and loaded them into liposomes, thereby taking advantage of the unique gene delivery properties of chitosan such as DNA condensation, protection against enzymatic degradation and enhancement in transfection efficiency (Cifani et al, 2015;Mao et al, 2010). The binding affinity of chitosan to pDNA as well as the capacity of the nanoparticles to protect pDNA against enzymatic degradation were evaluated.…”

Section: Introductionmentioning

confidence: 99%

Functionalized liposomal nanoparticles for efficient gene delivery system to neuronal cell transfection

Rodrigues

Banerjee

Kanekiyo

et al. 2019

International Journal of Pharmaceutics

View full text Add to dashboard Cite

Liposome based delivery systems provide a promising strategy for treatment of neurodegenerative diseases. A rational design of brain-targeted liposomes can support the development of more efficient treatments with drugs and gene materials. Here, we characterized surface modified liposomes with transferrin (Tf) protein and penetratin (Pen), a cell-penetrating peptide, for efficient and targeted gene delivery to brain cells. PenTf-liposomes efficiently encapsulated plasmid DNA, protected them against enzymatic degradation and exhibited a sustained in vitro release kinetics. The formulation demonstrated low cytotoxicity and was non-hemolytic. Liposomes were internalized into cells mainly through energy-dependent pathways especially clathrin-mediated endocytosis. Reporter gene transfection and consequent protein expression in different cell lines were significantly higher using PenTf-liposomes compared to unmodified liposomes. The ability of these liposome to escape from endosomes can be an important factor which may have likely contributed to the high transfection efficiency observed. Rationally designed bifunctional targeted-liposomes provide an efficient tool for improving the targetability and efficacy of synthesized delivery systems. This investigation of liposomal properties attempted to address cell differences, as well as, vector differences, in gene transfectability. The findings indicate that PenTf-liposomes can be a safe and non-invasive approach to transfect neuronal cells through multiple endocytosis pathways.

show abstract

Improved stability and efficacy of chitosan/pDNA complexes for gene delivery

Cited by 21 publications

References 14 publications

Ultrasonic compatibilization of polyelectrolyte complex based on polysaccharides for biomedical applications

Ultrasonic compatibilization of polyelectrolyte complex based on polysaccharides for biomedical applications

Chitosan-miRNA functionalized microporous titanium oxide surfaces via a layer-by-layer approach with a sustained release profile for enhanced osteogenic activity

Functionalized liposomal nanoparticles for efficient gene delivery system to neuronal cell transfection

Contact Info

Product

Resources

About