Multifaceted Applications of Chitosan in Cancer Drug Delivery and Therapy

Babu, Anish; Ramesh, Rajagopal

doi:10.3390/md15040096

Cited by 114 publications

(47 citation statements)

References 93 publications

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“…The results showed that Cs NPs have no effect on the DNA of BT breast cancer cell lines; with % DFI reach to 6.77%, which appear less than negative control 13.6% and in comparison with (++) positive control 36.0% and (++++) control 64.2%. This data suggests that chitosan NPs have no negative effect on the nucleic material of the BT breast cancer cell lines and can be used as gene delivery uptake and that approved with several other recent reported results that have been intensively studied for use of Cs NPs as carriers to deliver various drugs and genes in cancer treatment [31][32][33], and that will be focused on the therapeutic potential of chitosanbased nanoparticles for cancer treatment.…”

Section: Genotoxicity Assay (Dna Fragmentation)supporting

confidence: 68%

Comparative Cytotoxicity and Genotoxicity Assessments of Chitosan Amino Acid Derivative Nanoparticles Toward Human Breast Cancer Cell Lines

Zaboon

Saleh²,

Al-Lami

2019

Egypt. J. Chem.

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C HITOSAN, a natural macromolecule, is widely used in medical and pharmaceutical fields because of its distinctive properties such as a bactericide, fungicide and above all its antitumour effects. In this study; we aimed to develop an antitumour system based on Chitosan (Cs)and its some amino acid derivatives, namely Chitosan-Arginine (Cs-Arg) and Chitosan-Clycine-Aspartic acid (Cs-Gly-Asp)derivatives nanoparticles to improve their bioavailability and anticancer activity in antitumour treatments. The derivatives were obtained in a very good yield, and they were characterized by FTIR and some of them characterized by 1H-NMR, and the resulted spectra confirmed the right structures of synthesized chitosan derivatives. All the chitosan and its grafted amino acids were converted to nanoparticles in size by subjecting them to the sonication method. The scanning electron microscope (SEM) was used to determine the shape and size of the prepared polymeric nanoparticles, and they developed using the ImageJ program. The MTT assay and the flow cytometry technique for all prepared polymeric nanoparticles were determined against three different types of human breast cancer cell lines, and the results revealed the highly significant (p< 0.001), in the reducing of breast carcinoma viability in comparison with untreated cell lines, but the cytotoxicity effect of Cs-Arg nanoparticles were larger than Cs-Gly-Asp nanoparticles, whereas there was no genotoxicity effect against BT cell lines for the Cs-Gly-Asp nanoparticles and slight effect for Cs-Arg nanoparticles.

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Section: Genotoxicity Assay (Dna Fragmentation)supporting

confidence: 68%

Comparative Cytotoxicity and Genotoxicity Assessments of Chitosan Amino Acid Derivative Nanoparticles Toward Human Breast Cancer Cell Lines

Zaboon

Saleh²,

Al-Lami

2019

Egypt. J. Chem.

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“…CGRP is released from the pores in the solution with simple physical adsorption. Chitosan is widely used in medicine, food, and many other areas because of its characteristics as a drug carrier or accessory . Many studies on Chitosan and CPC composites show that Chitosan combined with CPC can improve the CPC surface brittleness .…”

Section: Discussionmentioning

confidence: 99%

Novel calcitonin gene‐related peptide/chitosan‐strontium‐calcium phosphate cement: Enhanced proliferation of human umbilical vein endothelial cells in vitro

Liang

et al. 2018

J Biomed Mater Res

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Bone cement materials have some disadvantages, including slow degradation and no biological activity, which greatly weakens their clinical application. Therefore, the search for a multifunctional bioactive bone cement has become urgent. In this study, a novel bone cement sample of calcitonin gene-related peptide (CGRP)/chitosan-strontium (Sr)-calcium phosphate cement (CPC) was developed. The structure and morphology were observed by scanning electron microscope (SEM). The cytotoxicity and proliferation of CGRP/chitosan-Sr-CPC were also measured. The expression of CGRP receptor 1 was measured using an immunofluorescence assay. Real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) were employed to quantify the VEGF mRNA and protein levels, respectively. Finally, the ability of the material to improve angiogenesis was assessed by using human umbilical vein endothelial cells (HUVECs) tube formation assay. The results showed that CGRP/Chitosan-Sr-CPC had the characteristics of a good orthopedic material without showing cell cytotoxicity to HUVECs. Meanwhile, CGRP/chitosan-Sr-CPC could release CGRP and enhance the proliferation of HUVECs via CGRP receptors. Moreover, CGRP/chitosan-Sr-CPC significantly upregulated the expression of the VEGF gene and protein in HUVECs, which might help improve the angiogenesis microenvironment. Besides, CGRP/chitosan-Sr-CPC could significantly improve angiogenesis of HUVECs. These findings provide new therapeutic material for the treatment of osteoporotic bone injury. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2018.

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“…The presence of numerous functional groups allows for easy modification for use in drug delivery applications. Chitosan nanoparticles can encapsulate chemotherapeutics, genes, peptides, or small molecules, and efficiently deliver the cargo for tumor therapy (Babu & Ramesh, 2017; Layek, Lipp, & Singh, 2015; Patel, Patel, & Patel, 2010). The cationic nature of chitosan is harnessed for gene delivery, as negatively charged nucleic acids readily form complexes with chitosan to form nanoparticles.…”

Section: Types Of Nanoparticles For Therapeutic Deliverymentioning

confidence: 99%

Recent Advances in Nanoparticle-Based Cancer Drug and Gene Delivery

Amreddy

Babu

Muralidharan

et al. 2018

Advances in Cancer Research

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222

145

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Effective and safe delivery of anticancer agents is among the major challenges in cancer therapy. The majority of anticancer agents are toxic to normal cells, have poor bioavailability, and lack in vivo stability. Recent advancements in nanotechnology provide safe and efficient drug delivery systems for successful delivery of anticancer agents via nanoparticles. The physicochemical and functional properties of the nanoparticle vary for each of these anticancer agents, including chemotherapeutics, nucleic acid-based therapeutics, small molecule inhibitors, and photodynamic agents. The characteristics of the anticancer agents influence the design and development of nanoparticle carriers. This review focuses on strategies of nanoparticle-based drug delivery for various anticancer agents. Recent advancements in the field are also highlighted, with suitable examples from our own research efforts and from the literature.

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Multifaceted Applications of Chitosan in Cancer Drug Delivery and Therapy

Cited by 114 publications

References 93 publications

Comparative Cytotoxicity and Genotoxicity Assessments of Chitosan Amino Acid Derivative Nanoparticles Toward Human Breast Cancer Cell Lines

Comparative Cytotoxicity and Genotoxicity Assessments of Chitosan Amino Acid Derivative Nanoparticles Toward Human Breast Cancer Cell Lines

Novel calcitonin gene‐related peptide/chitosan‐strontium‐calcium phosphate cement: Enhanced proliferation of human umbilical vein endothelial cells in vitro

Recent Advances in Nanoparticle-Based Cancer Drug and Gene Delivery

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