Chitosan and Pichia membranaefaciens control anthracnose by maintaining cell structural integrity of citrus fruit

Zhao, Yijie; Deng, Lili; Zhou, Yahan; Yao, Shixiang; Zeng, Kaifang

doi:10.1016/j.biocontrol.2018.05.004

Cited by 29 publications

(18 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…). Massive accumulation of ROS levels usually accelerates the occurrence of lipid peroxidation in both cellular and organelle membranes, which, in turn, affects normal cellular function (Tripathi and Rasheed, ; Zhao et al ). Therefore, these results suggest that GA addition can lead to membrane damage in G. citri‐aurantii through lipid peroxidation.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effects of glutaraldehyde on Geotrichum citri‐aurantii and its possible mechanism

Yang

Ouyang

et al. 2019

J Appl Microbiol

View full text Add to dashboard Cite

Aims This study investigated the inhibitory effect of glutaraldehyde (GA) on sour rot in citrus fruit and the underlying antifungal mechanism on mycelial growth of the causative pathogen Geotrichum citri‐aurantii. Methods and Results Glutaraldehyde exhibited a strong inhibitory effect on G. citri‐aurantii, with a minimum inhibitory and fungicidal concentration (MFC) of 1·00 µl ml−1. In addition, in vivo application of GA (1 × MFC and 5 × MFC) reduced the disease incidence of sour rot in citrus fruit by 60 and 80% respectively. Scanning electron microscopy results revealed that the morphology of G. citri‐aurantii mycelia was greatly altered by GA treatment. Propidium iodide and Calcofluor White Staining revealed that the membrane permeability, rather than the cell wall integrity, of G. citri‐aurantii mycelia was severely disrupted after the addition of GA. Massive accumulation of malonaldehyde and reactive oxygen species as well as an increase in lipoxygenase activity were observed. Conclusion These results indicate that GA may inhibit the mycelia growth of G. citri‐aurantii through a membrane damage mechanism induced by membrane peroxidation. Significance and Impact of the Study Glutaraldehyde is expected to be a novel fungicide for controlling sour rot in citrus fruit.

show abstract

Section: Discussionmentioning

confidence: 99%

Inhibitory effects of glutaraldehyde on Geotrichum citri‐aurantii and its possible mechanism

Yang

Ouyang

et al. 2019

J Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…In our study, decreased hardness and increased electrical conductivity of banana fruits were observed when the banana fruit was treated with a banana anthracnose conidia suspension. Anthracnose infection that causes cell membrane destruction and cell wall degradation has been well demonstrated in previous studies (Xu et al, 2017;Zhao, Deng, Zhou, Yao, & Zeng, 2018). The characteristics of fruit hardness and electrical conductivity have been widely used as indexes to evaluate fruit quality (Zhang, 2010).…”

Section: Discussionmentioning

confidence: 95%

Role of phospholipase C in banana in response to anthracnose infection

Shuai

Jian

et al. 2020

Food Science & Nutrition

View full text Add to dashboard Cite

Phospholipase C (PLC) plays an important role in plant immunity, and anthracnose caused by the Colletotrichum species is a common postharvest disease of the banana fruit. This study aims to evaluate the role of PLC in anthrax resistance in banana. The experimental group of banana samples was treated with a banana anthracnose conidia suspension, and the control group was treated with distilled water. After inoculation, the groups were sprayed with ethephon, and indicators, such as hardness and conductivity changes; PLC activity, 1,2‐diacylglycerol (DAG) and phosphatidic acid (PA)content; and MaPLC‐1and MaPLC‐2 expression levels, were assessed at 0, 3, 6, 9, 12, and 15 days. Moreover, the expression levels of MaPLC‐1 and MaPLC‐2 were detected in various tissues. The hardness of banana fruits in the experimental group decreased faster than that in the control group. Furthermore, the conductivity was higher in the experimental group than in the control group. Regarding PLC activity, DAG, and PA content, bananas in the experimental group showed higher activities than those in the control group. Moreover, relatively higher expression of PLC mRNA was detected in anthracnose‐inoculated tissues. The evaluation of MaPLC‐1 and MaPLC‐2 expression levels showed that the mature peel had the highest MaPLC‐1 expression level. However, the MaPLC‐2 gene was expressed at relatively low levels in the fruit and at relatively high levels in the flower organs. PLC might play a role in fruit ripening in response to anthracnose resistance.

show abstract

“…Several studies have demonstrated that cell wall compounds can be affected by fungi infection [29,34]. Pectin is a cell wall polysaccharide in plants, including fruit crops [35] and pectin is an essential compound in the cell wall maintaining the structural integrity [36].…”

Section: Discussionmentioning

confidence: 99%

“…Three gams of mandarin peel samples from thirty fruit were used for polygalacturonase (PG, EC 3.2.1.15) and pectin methylesterase (PME, EC 3.1.1.11) activities measurement using the approaches described by Zhao et al [34].…”

Section: Methodsmentioning

confidence: 99%

Mechanism of Cell Wall Polysaccharides Modification in Harvested ‘Shatangju’ Mandarin (Citrus reticulate Blanco) Fruit Caused by Penicillium italicum

Shi

et al. 2019

Biomolecules

View full text Add to dashboard Cite

Modification of cell wall polysaccharide in the plant plays an important role in response to fungi infection. However, the mechanism of fungi infection on cell wall modification need further clarification. In this study, the effects of Penicillium italicum inoculation on ‘shatangju’ mandarin disease development and the potential mechanism of cell wall polysaccharides modification caused by P. italicum were investigated. Compared to the control fruit, P. italicum infection modified the cell wall polysaccharides, indicated by water-soluble pectin (WSP), acid-soluble pectin (ASP), hemicellulose and lignin contents change. P. italicum infection enhanced the activities of polygalacturonase (PG), pectin methylesterase (PME), and the expression levels of xyloglucanendotransglucosylase/hydrolase (XTH) and expansin, which might contribute to cell wall disassembly and cellular integrity damage. Additionally, higher accumulation of reactive oxygen species (ROS) via decreasing antioxidant metabolites and the activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) also contributed to the cell wall polysaccharides modification. Meanwhile, the gene expression levels of hydroxyproline-rich glycoprotein (HRGP) and germin-like protein (GLP) were inhibited by pathogen infection. Altogether, these findings suggested that cell wall degradation/modification caused by non-enzymatic and enzymatic factors was an important strategy for P. italicum to infect ‘shatangju’ mandarin.

show abstract

Chitosan and Pichia membranaefaciens control anthracnose by maintaining cell structural integrity of citrus fruit

Cited by 29 publications

References 54 publications

Inhibitory effects of glutaraldehyde on Geotrichum citri‐aurantii and its possible mechanism

Inhibitory effects of glutaraldehyde on Geotrichum citri‐aurantii and its possible mechanism

Role of phospholipase C in banana in response to anthracnose infection

Mechanism of Cell Wall Polysaccharides Modification in Harvested ‘Shatangju’ Mandarin (Citrus reticulate Blanco) Fruit Caused by Penicillium italicum

Contact Info

Product

Resources

About