Chitosan–aluminum monostearate composite sponge dressing containing asiaticoside for wound healing and angiogenesis promotion in chronic wound

Phaechamud, Thawatchai; Yodkhum, Kotchamon; Charoenteeraboon, Juree; Tabata, Yasuhiko

doi:10.1016/j.msec.2015.02.003

Cited by 54 publications

(30 citation statements)

References 53 publications

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“…Immunohistochemical examination showed increased expression of AQP3 in the membrane of NHEKs after exposure to the Piptadenia colubrina extract at a concentration of 10 mg/ mL for 24 hours compared to the control group without treatment. (18) In another study by Pereda et al (20) an extract of green Coffea arabica seed showed increased AQP3 expression in the normal epidermal layer of human skin. NHEKs were incubated for 3-6 hours with the optimal concentration of 25 mg/mL.…”

Section: Discussionmentioning

confidence: 93%

“…(19) From research on the induction of AQP3 by Piptadenia colubrina extract at concentrations of 10 and 20 mg/mL in cultured human skin cells it was found that the extract significantly increased the AQP3 mRNA expression as analyzed by real time-polymerase chain reaction (RT-PCR). (18) AQP3 began to increase after 2 hours, reached its peak at 6 hours, and decreased after 24 hours. Immunohistochemical examination showed increased expression of AQP3 in the membrane of NHEKs after exposure to the Piptadenia colubrina extract at a concentration of 10 mg/ mL for 24 hours compared to the control group without treatment.…”

Section: Discussionmentioning

confidence: 94%

“…Included in this list of active compounds are those from Coffea Arabica, (17) Piptadenia colubrina, (18) and Ajuga turkestanica. (19) From research on the induction of AQP3 by Piptadenia colubrina extract at concentrations of 10 and 20 mg/mL in cultured human skin cells it was found that the extract significantly increased the AQP3 mRNA expression as analyzed by real time-polymerase chain reaction (RT-PCR).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore chitosan-aluminum monostearate composite sponge dressings containing asiaticoside may be of benefit for wound healing and angiogenesis promotion in chronic wounds. (18) There has been no studies on the expression of AQP3 in NHEKs after treatment with asiaticoside as compared with that of RA. The aim of this study was to evaluate and compare the effect of asiaticoside isolated from CA and that of RA on the expression of AQP3 in NHEKs.…”

Section: Original Article Introductionmentioning

confidence: 99%

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Asiaticoside increases aquaporin-3 protein expression in the cytoplasm of normal human epidermal keratinocytes

Wijayadi

Darmawan

2017

UnivMed

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Introduction Skin hydration decreases with aging. Aquaporin-3 (AQP3) is a major protein that plays a role in skin hydration, therefore it is a novel target for skin moisturizing treatment. Retinoic acid (RA) as a well-known active agent in antiaging treatment increases AQP3 expression, but frequently causes harmful side effects. Asiaticoside, a saponin compound isolated from Centella asiatica (CA) is also known as an antiaging cosmetic and plays a role in wound healing. The aim of this study was to evaluate and compare the effect of asiaticoside isolated from CA and the effect of RA on the AQP3 expression in normal human epidermal keratinocytes (NHEKs).Methods An experimental laboratory study was performed using primary NHEKs that were derived from the foreskin of a boy. AQP3 expression in NHEKs was examined in vitro after the cells were incubated for 24 hours with asiaticoside or with RA at several concentrations. The AQP3 expression was evaluated by immunocytochemistry and quantitatively analyzed by Image-J software. Independent t-test and one-way ANOVA were used to analyze the data, followed by post-hoc Tukey test.Results There was an increasing trend of AQP3 expression upon exposure to asiaticoside at all concentrations compared to the control group. However, RA exposure seemed to induce a higher level of AQP3 expression. Asiaticoside effected a lower increase in AQP3 expression in NHEKs than did RA (p=0.042). Optimal results were achieved at 1 mg/mL concentration of asiaticoside.Conclusions Asiaticoside isolated from CA can enhance the AQP3 expression in NHEKs. Therefore it can be used as an active ingredient in cosmetic moisturizer formulation for dry skin treatment.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Original Article Introductionmentioning

confidence: 99%

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Asiaticoside increases aquaporin-3 protein expression in the cytoplasm of normal human epidermal keratinocytes

Wijayadi

Darmawan

2017

UnivMed

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“…Chronic wounds, including arterial ulcers, pressure ulcers, venous ulcers and diabetic foot ulcers, are defined as tissue injuries that do not heal in an orderly set of stages and that also do not heal within 12 weeks [1][2][3][4]. In particular, diabetic foot ulcers are one of the major complications associated with diabetic mellitus.…”

Section: Introductionmentioning

confidence: 99%