Chitinolytic properties of Bacillus pabuli K1

Frändberg, Emma; Schnürer, Johan

doi:10.1111/j.1365-2672.1994.tb01641.x

Cited by 49 publications

(30 citation statements)

References 26 publications

(22 reference statements)

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“…This result is consistent with patterns of chitinase production for Bacillus spp. (Priest, 1977 ;Frandberg & Schnurer, 1994) and the fungus Metarhizium anisopliae (Kang et al, 1999). The highest rates of chitinase production between 120 and 192 h were found when the culture supernatant from I3 was tested for endo-and exo-acting activity (data not shown).…”

Section: Chitinase Activity Of Bacterial Isolates (External Pathogenimentioning

confidence: 99%

Shell disease syndrome in the edible crab, Cancer pagurus – isolation, characterization and pathogenicity of chitinolytic bacteria

2002

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Chitinolytic bacteria are believed to be the primary aetiological agents of shell disease syndrome in marine crustaceans. The disease principally results from the breakdown of their chitinous exoskeletons by the shell disease pathogens, but pathogenicity may also manifest internally should a breach of the carapace occur. The current study looks at the pathogenicity of a number of bacteria (predominantly from the genus Vibrio) isolated from the edible crab, Cancer pagurus. All chitinase-producing bacteria investigated were capable of growth in a minimal medium consisting of chitin powder from crab shells, but differed in their speed of growth and nature of chitinolytic activity, suggesting that they have different roles within the lesion community. Two isolates (designated I4 and I7) were chosen for studies on internal pathogenicity, which included the effect of the pathogen on crab tissues, the ability of the host to remove the bacteria from circulation and the antibacterial activity of crab blood. Initially, I4 was rapidly removed from circulation, but began to reappear in the blood after 24 h. By 100 h, 100 % of crabs were moribund. The septicaemic effects of the isolate were reflected in the low levels of its killing by blood-cell lysate and serum. By contrast, I7 was only slowly removed from circulation and caused the rapid mortality of all crabs in T 3 h. A large decline in the number of circulating blood cells following injection of I7 was mirrored by an accumulation of these cells in the gills. Initial experiments suggest that the death of the crabs following injection with I7 may be caused by toxic extracellular bacterial products that exert their effects on the blood cells and nervous system of the crabs.

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Section: Chitinase Activity Of Bacterial Isolates (External Pathogenimentioning

confidence: 99%

Shell disease syndrome in the edible crab, Cancer pagurus – isolation, characterization and pathogenicity of chitinolytic bacteria

2002

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“…In contrast, this compound acts as a repressor of chitinase gene expression in Serratia marcescens (54). Also, in Bacillus pabuli, (GlcNAc) [2][3][4] induces the production of chitinases, whereas no chitinases could be detected in the presence of GlcNAc (14). We show here that the two chitinase genes in L. monocytogenes, chiA and chiB, were induced by different substrates, as only chiA was induced by the monomer GlcNAc, whereas the expression of chiB depended on chitin oligosaccharides present in the chitin suspension.…”

Section: Vol 76 2010mentioning

confidence: 99%

“…and Streptomyces lividans (14,36). A microarray study found that lmo0106 and, to a lesser degree, lmo1883 (chiA) are upregulated in two mutants defective in carbon catabolite control, the hprK and ccpA insertion mutants, indicating that lmo0106 presumably is repressed by CcpA/HPr(Ser-P) in the presence of glucose (32).…”

Section: Vol 76 2010mentioning

confidence: 99%

The Chitinolytic Activity ofListeria monocytogenesEGD Is Regulated by Carbohydrates but Also by the Virulence Regulator PrfA

Larsen

Leisner

Ingmer

2010

Appl Environ Microbiol

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Chitin, an insoluble polymer of N-acetyl-D-glucosamine (GlcNAc), is one of the most abundant carbohydrate polymers in marine and terrestrial environments. Chitin hydrolysis by Listeria monocytogenes depends on two chitinase-encoding genes, chiA and chiB, and the aim of this study was to investigate their regulation. Chitin induces the expression of both chitinases in late exponential growth phase, and chiA but not chiB is furthermore induced by the monomer GlcNAc. Furthermore, their expression is subjected to catabolite control. Chitinases expressed by bacterial pathogens have proven to be important not only for nutrient acquisition and environmental survival but also for infecting animals and humans. Interestingly, the central L. monocytogenes virulence gene regulator, PrfA, is required for the chitinolytic phenotype, as chitinase activity was significantly reduced in prfA mutant cells compared to its level in wild-type cells. In agreement with this, Northern blot analysis showed that the amounts of chiA and chiB transcripts upon induction by chitin were significantly lower in the prfA mutant than in the wild type. The chitinolytic activity and chiA and chiB expression were reduced in the absence of the sigB gene, indicating that B is also important for the production of chitinases. The chiA, chiB, and chiA chiB mutants were not impaired for in vitro adhesion and invasion in epithelial cell lines, but the chiA chiB double mutant showed less survival ability in a chitin-enriched medium. The regulation of chitinolytic activity in L. monocytogenes is complex, and taken together, the results indicate that the biological role of this activity may not be limited to the external environment.

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“…Bacillus sp., and Serratia sp have a chitinase producing ability [3][4][5]. It showed pesticidal effects against a number of plant pathogens including Rhizoctonia solani and Fusarium oxysporium causing wilt disease [6].…”

Section: Introductionmentioning

confidence: 99%

Isolation of Serratia marcescens SR1 as a Source of Chitinase Having Potentiality of Using as a Biocontrol Agent

Parani¹,

Shetty²,

Saha³

2011

Indian J Microbiol

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Serratia marcescens, strain SR 1 was isolated from the local soil of a cultivated farm and it was screened as potent strain for chitinase production. Maximum chitinase production (77.3 u Mh -1 100 -1 ) was observed after 96 h of incubation period with pH 5.5 at 30°C under shake conditions (120 rpm). Compare to still flasks, shake culture with prawn fish colloidal chitin of 0.5% (w/v) concentration, showed a better enzyme yield. Crude enzyme showed antifungal activity against plant pathogens.

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Chitinolytic properties of Bacillus pabuli K1

Cited by 49 publications

References 26 publications

Shell disease syndrome in the edible crab, Cancer pagurus – isolation, characterization and pathogenicity of chitinolytic bacteria

Shell disease syndrome in the edible crab, Cancer pagurus – isolation, characterization and pathogenicity of chitinolytic bacteria

The Chitinolytic Activity ofListeria monocytogenesEGD Is Regulated by Carbohydrates but Also by the Virulence Regulator PrfA

Isolation of Serratia marcescens SR1 as a Source of Chitinase Having Potentiality of Using as a Biocontrol Agent

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