Chitin isolates: Species variation in residual amino acids

Brine, Charles J.; Austin, Paul R.

doi:10.1016/0305-0491(81)90031-6

Cited by 22 publications

(13 citation statements)

References 9 publications

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“…The presence of chitin-protein complexes was confirmed by the complementary characterization of short glycopeptides (Lipke and Geoghegan, 1971;Spiro, 1966). However, despite an increasing number of reports on chitin-protein complexes in various species, the discrepancy in the amount and composition of amino acids found between even closely related species cannot be accounted for (Brine and Austin, 1981;Hunt and Huckerby, 1987). Importantly, there has been no systematic study of the molecular nature of chitin-protein complex formation in mollusc shells.…”

Section: Introductionmentioning

confidence: 74%

Covalent modification of chitin with silk-derivatives acts as an amphiphilic self-organizing template in nacre biomineralisation

Weiss

Kaufmann

Heiland

et al. 2009

Journal of Structural Biology

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Section: Introductionmentioning

confidence: 74%

Covalent modification of chitin with silk-derivatives acts as an amphiphilic self-organizing template in nacre biomineralisation

Weiss

Kaufmann

Heiland

et al. 2009

Journal of Structural Biology

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“…Another possibility is that the chitin is partially degraded to some form that is not easily hydrolyzed by chitinase but still binds with WGA. Chitin produced by marine invertebrates is cross-linked with proteins (Austin et al 1981, Brine & Austin 1981. Chitinolytic marine bacteria could degrade and utilize the portions of the chitin fibrils that were susceptible to hydrolysis by chitinase.…”

Section: Discussionmentioning

confidence: 99%

A simple assay for chitin application to sediment trap samples from the subarctic Pacific

Montgomery¹,

Welschmeyer²,

Kirchman³

1990

Mar. Ecol. Prog. Ser.

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Chitin is thought to be abundant in marine environments, but examination of the role of chitin in nutrient cycling has been hampered by the lack of an adequate assay for measuring concentrations at ambient levels. We developed a simple assay using the lectin, wheat germ agglutinin (WGA), which has an affinity for the N-acetylglucosamine (NAG) residues found in chitin. The specificity of the assay was confirmed by enzymatic hydrolysis with chitinase and competitive inhibition with chltotriose. The lectin bound specifically to chitin even when samples contained high concentrations of cellulose, clay, and bacteria. Concentrations of suspended chitin were 4 to 21 pg 1-' in Delaware Bay, USA, and 4 to 10 pg 1-' in the subarctic Pacific. The assay was also applied to sediment trap samples collected in the subarctic Pacific. We found that the chitin flux accounted for less than 1 % of carbon and nitrogen fluxes above 500 m. Using fluorescently-labelled WGA and epifluorescence microscopy, we were able to differentiate detritus, zooplankton fecal pellets and possibly fungi from nonchltinous particles.

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“…These residues are considerably lower than those (44.6 -47.4 mg/g) reported by Sophanodora and Hutadilok (1995) for black tiger shrimp chitosan. Differences in residual amino acids of chitosans reported in our study and by Sophanodora and Hutadilok (1995) probably are due to different preparation methods applied or to speciesrelated variability (Brine and Austin 1981b).…”

Section: Characteristics Of Chitosan Productsmentioning

confidence: 95%

Correlation Between Physicochemical Characteristics and Binding Capacities of Chitosan Products

No¹,

Lee²,

Meyers³

2000

Journal of Food Science

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Physicochemical characteristics (molecular weight, nitrogen, ash, degree of deacetylation, bulk density, viscosity, and residual amino acids) of 6 commercial chitosans differed with products. Significant correlations were observed between molecular weight and viscosity and between nitrogen and degree of deacetylation. Binding capacities of water, fat, and three different dyes also varied depending on the products. Results of correlations between binding capacities and physicochemical characteristics indicated that both water and fat binding capacities were significantly negatively correlated with bulk density. In addition, fat binding capacity showed a significant correlation with viscosity. Significant negative correlations were observed between dye binding capacities of three red, yellow, and blue dyes and ash.

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Chitin isolates: Species variation in residual amino acids

Cited by 22 publications

References 9 publications

Covalent modification of chitin with silk-derivatives acts as an amphiphilic self-organizing template in nacre biomineralisation

Covalent modification of chitin with silk-derivatives acts as an amphiphilic self-organizing template in nacre biomineralisation

A simple assay for chitin application to sediment trap samples from the subarctic Pacific

Correlation Between Physicochemical Characteristics and Binding Capacities of Chitosan Products

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