Chitin is thought to be abundant in marine environments, but examination of the role of chitin in nutrient cycling has been hampered by the lack of an adequate assay for measuring concentrations at ambient levels. We developed a simple assay using the lectin, wheat germ agglutinin (WGA), which has an affinity for the N-acetylglucosamine (NAG) residues found in chitin. The specificity of the assay was confirmed by enzymatic hydrolysis with chitinase and competitive inhibition with chltotriose. The lectin bound specifically to chitin even when samples contained high concentrations of cellulose, clay, and bacteria. Concentrations of suspended chitin were 4 to 21 pg 1-' in Delaware Bay, USA, and 4 to 10 pg 1-' in the subarctic Pacific. The assay was also applied to sediment trap samples collected in the subarctic Pacific. We found that the chitin flux accounted for less than 1 % of carbon and nitrogen fluxes above 500 m. Using fluorescently-labelled WGA and epifluorescence microscopy, we were able to differentiate detritus, zooplankton fecal pellets and possibly fungi from nonchltinous particles.
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