Chitin- And Chitosan Biosorbents From Citric a Cid Mycelial Industrial Waste

Новинюк, Л. В.; Кулёв, Д. Х.; Negrutsa, I.; Велинзон, П. З.

doi:10.21323/2618-9771-2018-1-2-55-62

Cited by 3 publications

(4 citation statements)

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“…It is possible that at an earlier stage of the process the content of β-glucans is different and, accordingly, the ratio of glucan and chitin components of HGC, occupying 40 -50 % of the dry matter of the cell wall, also changes. Earlier studies have shown that successive alkaline and acidic effects on Aspergillus biomass lead to deproteinization, deacetylation and ensure the availability of chitin and glucan copolymers of HGC for enzymes with different specificity of action [19]. Comparatively, exposure to endo-(1→3)(1→4)-β-glucanase T. Longibrachiatum, which has the specificity of action to non-starch polysaccharides (hemicelluloses and cellulose cleavage products), is more pronounced in HGC from biomass obtained during fermentation of starch-containing medium.…”

Section: Discussionmentioning

confidence: 99%

Beta-glucans from biomass of plant and microbial origin

Sharova¹,

Manzhieva

Принцева³

et al. 2019

Food systems

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The aim of the present study is to explore the transformation of (1→3)(1→4)-β-D-glucans of rye biomass byAspergills niger and accumulation of (1→3)(1→6)-β-D-glucans in the microbial cell wall.Biomass from rye grain was obtained as a result of enzymatic hydrolysis of grain grinding of Omsk region of non-standard quality with grain impurity content of 45 ± 2 % by preparations (1→4)-β-glucanolytic, (1→3)-β-glucanolytic, (1→4)-xylanolytic and (1→4)-amylolytic action. Fermentation of hydrolysates, sucrosemineral and molasses medium by A. niger was carried out by a batch process under aerobic conditions. Determined the content of β-glucans, amino-nitrogen, glucose, disaccharides in grinding grain rye, rye biomass, the biomass of A. niger, the supernatants by colorimetric methods. Determination of chitin in biomass and qualitative determination of chitosan in supernatants of hydrolysates was carried out using chitosan sulfate sample and subsequent microscopy.The results of the research showed that (1→3)(1→4)-β-D-glucans in grain grinding are 10.2 ± 0.2 % in terms of dry matter, which exceeds the content of polysaccharide in the grain of standard quality by 1.5 – 3 times. In rye biomass revealed their smaller amount, 6.4 ± 0.5 %, apparently, due to the action of (1→4)and (1→3)-β-glucanase, (1→4)-xylanase and (1→4)-amylase. In microbial mass A. niger content of (1→3)(1→6)-β-Dglucans were at the level of 21.7 ± 0.7 %.On the basis of the obtained results, it was concluded that it is possible to use rye grain of non-standard quality, with a high content of grain impurities and a low proportion of starch polysaccharides, as a source of β-glucancontaining substrate for biosynthesis (1→3)(1→6)-β-D-glucans by A. niger having advantages over (1→3) (1→4)-β-D-glucans of plant origin. They are functionally more active and have a wide range of applications, namely as food additives in the manufacture of a wide range of products: for the enrichment of fibers, increasing the shelf life of products due to its water-binding properties, as thickeners, emulsifying and fat-reducing microingredients, stabilizers of creamy emulsions, textureformers, flavor enhancers.

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Section: Discussionmentioning

confidence: 99%

Beta-glucans from biomass of plant and microbial origin

Sharova¹,

Manzhieva

Принцева³

et al. 2019

Food systems

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“…В качестве объектов исследования использованы образцы хитозана, полученные по разработанному способу [13] из мицелиальной биомассы гриба Aspergillus niger. Клеточная стенка гриба Aspergillus niger содержит хитин-глюкановый комплекс, из которого выделен хитозан.…”

Section: экспериментальная частьunclassified

“…Наиболее изучено ММР хитозана, полученного из панцирей ракообразных [9,10]. Нами разработана схема получения хитозана из мицелиальной массы гриба Aspergillus niger -продуцента лимонной кислоты [11]. Исследование его молекулярно-массовых характеристик представляет собой научный и практический интерес.…”

Section: Introductionunclassified

Изучение молекулярно-массового распределения хитозана из мицелия гриба Aspergillus niger методом гель-хроматографии

Велинзон¹,

Novinyuk²

2020

sorpchrom

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В статье представлены результаты изучения молекулярного состава хитозана, выделенного из мицелия гриба Aspergillus niger, в сравнении с образцом фирмы Fratelli Parodi (контроль). Хитозан представляет собой сополимер глюкозамина и ацетилглюкозамина и является полимолекулярным биополимером. Возможности применения хитозана во многом определяются его сорбционной и био-логической активностью, которая, в значительной степени, зависит от молекулярной массы. Целью работы является изучение молекулярно-массового распределения (ММР) полимерных молекул хито-зана с использованием метода гель-проникающей хроматографии.Получены хроматограммы выхода полимерных молекул изученных образцов хитозана, на ко-торых наблюдается наличие 3-х характерных пиков, соответствующих трём основным фракциям: вы-сокомолекулярной (ММ 34-105 кДа), фракции со средней молекулярной массой (ММ 17.5-27.5 кДа) и низкомолекулярной (ММ 4.5-13.5 кДа). Установлен молекулярно-массовый состав, определена сред-немассовая и среднечисловая молекулярная масса отдельных фракций хитозанов.Построены кривые ММР, характеризующие молекулярно-массовое распределение по фрак-циям. Анализ кривых ММР показывает, что полученный из мицелия гриба Aspergillus niger и кон-трольный образцы, имеют относительно идентичный полимолекулярный состав. Основную долю со-ставляет высокомолекулярная фракция около 60%. Массовая доля фракции со средней молекулярной массой в контрольном образце хитозана составляет 20%, а в опытном – 10%. Доля низкомолекуляр-ной фракции в контрольном образце хитозана составляет около 20%, а в опытном – 30%. При этом показано, что дисперсия молекулярных масс близка к 1, что характерно для мономолекулярных био-полимеров.

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“…Chitosan -poly-β-(1,4)-d-glucosamine is a deacetylated product of chitin -poly-β- (1,4)-N-acetyl-d-glucosamine, one of the most abundant natural polysaccharide, found in composition of crustacean shells and fungal cell walls [1]. There is great scientific and practical interest in chitin and chitosan caused to the unique properties as biocompatibility, safety, biodegradability, sorption performance of heavy metals and radionuclides [2]. Scientific publications amount related to chitin and chitosan is increasing annually and several scientific papers related to its production and application are published weekly according to the Web of Science and Google Scholar.…”

Section: Introductionmentioning

confidence: 99%

Chitosan Application in Food Technology: A Review of Rescent Advances

Kabanov

Novinyuk

2020

Food systems

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A BST R ACTThe review focused on global trends in the development of scientific research and the practical applications of chitosan in food technology in recent years. Chitin and its derivative chitosan obtaining from the crustacean shells and the cell wall of fungi are among the most common biopolymers in the world. Chitosan is a polysaccharide discerned by a large number of unsubstituted amino groups. Featured properties of chitosan providing its high chemical and biological activities. Chitosan has various abilities as polycationite, film former, antimicrobial and antioxidant agent. Multifunctional properties open up broad prospects for the chitosan applications in various fields of technology, medicine and industry. The most attention in the review is paid to the works on extending food products shelf life with chitosan based primary edible film coatings and biodegradable packaging. At the same time chitosan applications as an emulsifier, a flocculant, as well as functional food additive, nutrient encapsulating material and dietary supplement are highlighted.

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Chitin- And Chitosan Biosorbents From Citric a Cid Mycelial Industrial Waste

Cited by 3 publications

References 6 publications

Beta-glucans from biomass of plant and microbial origin

Beta-glucans from biomass of plant and microbial origin

Изучение молекулярно-массового распределения хитозана из мицелия гриба Aspergillus niger методом гель-хроматографии

Chitosan Application in Food Technology: A Review of Rescent Advances

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