Chiral resolution of atropine, homatropine and eight synthetic tropinyl and piperidinyl esters by capillary zone electrophoresis with cyclodextrin additives

Jin, Lian; Yan, Wen; Xu, Rong; Go, Mei‐Lin; Lee, Hian Kee; Li, Sam Fong Yau

doi:10.1002/(sici)1522-2683(19990101)20:1<198::aid-elps198>3.0.co;2-7

Cited by 12 publications

(3 citation statements)

References 23 publications

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“…Our previous work [27] proved that a 1:2 ratio was sufficient for removing interferences in a direct UV detection mode. In this study, a 1:2 serum to acetonitrile ratio was initially tried and found to be insufficient for removing a broad negative peak that interfered with VPA and IS detection.…”

Section: Serum Deproteinizationmentioning

confidence: 96%

Indirect laser-induced fluorescence detection of valproic acid in human serum by capillary electrophoresis

Jin

Wang

1999

Electrophoresis

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A capillary electrophoresis (CE) method with indirect laser‐induced fluorescence detection for the analysis of valproic acid in human serum has been explored. The buffer system was optimized with 2.5 mM borate‐phosphate at pH 8.4; fluorescein sodium was used to generate background signal at a concentration of 6 μM. Hexanoic acid was selected as internal standard. Serum sample was deproteinized by acetonitrile. Analysis was performed by direct injection of the supernatant. CE separation was carried out at 30 kV and the total analysis time was less than 15 min, including sample treatment and electrophoresis time. No interference from other common anticonvulsant drugs occurred under the experimental conditions used. The interference of human serum matrix was reduced by using a high ratio of acetonitrile to serum (minimum 5:1) for deproteinization. Interference of ionic components in serum could occur, depending on the sample source. The linear range of concentrations for standard drug was between 4.5—144.0 μg/mL (r = 0.9947). The limit of detection was 0.9 μg/mL at S/N ≥ 3; the limit of quantitation at S/N ≥ 20 was 3 μg/mL. The recoveries of valproic acid spiked into serum were 69.2% and 60.2% for concentration levels of 90 and 54 μg/mL, respectively. This CE method was shown to be successful in the analysis of valproic acid in standard solutions. However, interference from the matrix was observed in the analysis of this compound in serum samples. Additional work should be done to develop a highly selective sample preparation technique.

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Section: Serum Deproteinizationmentioning

confidence: 96%

Indirect laser-induced fluorescence detection of valproic acid in human serum by capillary electrophoresis

Jin

Wang

1999

Electrophoresis

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“…CE as a microanalytical system can meet most of these requirements. However, few works report the application of CE in analysis of biological fluids [67,92,118,123,163,169,280,281]. There are some problems associated with bioanalysis, (i) complexity of sample matrix, containing high amounts of electrolytes and proteins, which can easily be adsorped onto the hydrophobic and negatively charged surface of the capillary and clog the capillary or affect the EOF, and (ii) relatively poor LOD achievable by CE.…”

Section: Applications Of Chiral Ce To Biomedical Analysismentioning

confidence: 99%

“…TM-b-CD dissolved in a Tris-phosphate buffer (50 mM and pH 2.8) was used for stereoselective bioanalysis of atropine in human serum [67]. Enantiomers of atropine at a concentration of 25 mg/mL spiked in human serum were separated in a fused silica capillary with no pretreatment of the sample matrix.…”

Section: Applications Of Chiral Ce To Biomedical Analysismentioning

confidence: 99%

Recent developments in chiral capillary electrophoresis and applications of this technique to pharmaceutical and biomedical analysis

Ahmad

2001

Electrophoresis

186

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This paper provides an overview of the current status of chiral capillary electrophoresis (CE). The emphasis is placed on the application of CE in chiral separation of various racemic compounds. During the last two years about 280 papers, several review articles, and two entire issues, edited by S. Fanali (Electrophoresis 1999, 20, 2577-2798, and H. Nishi and S. Terabe (J. Chromatogr. A 2000, 879, 1-471.) have been devoted to chiral CE. Enantiomeric separations of various compounds, e.g., pharmaceuticals, drug candidates, drugs and related metabolites in biological fluids, amino acids, di- and tri peptides, pesticides and fungicides, have been performed using different chiral selectors. Native and derivatized cyclodextrins continue to be the most widely used chiral selectors. Other chiral selectors such as natural and synthetic chiral micelles, crown ethers, chiral ligands, proteins, oligo- and polysaccharides, and macrocyclic antibiotics have also been applied to chiral CE separations.

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Complexation of tropane alkaloids by cyclodextrins

Asztemborska

Ceborska

Pietrzak

2019

Carbohydrate Polymers

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Chiral resolution of atropine, homatropine and eight synthetic tropinyl and piperidinyl esters by capillary zone electrophoresis with cyclodextrin additives

Cited by 12 publications

References 23 publications

Indirect laser-induced fluorescence detection of valproic acid in human serum by capillary electrophoresis

Indirect laser-induced fluorescence detection of valproic acid in human serum by capillary electrophoresis

Recent developments in chiral capillary electrophoresis and applications of this technique to pharmaceutical and biomedical analysis

Complexation of tropane alkaloids by cyclodextrins

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