Chinese guidelines on the clinical application of 5-aminolevulinic acid-based photodynamic therapy in dermatology (2021 edition)

Shi, Lei; Wang, Hongwei; Chen, Kun; Jia, Yan; Yu, Bo; Wang, Shengchun; Yin, Rui; Nong, Xiang; Zou, Xianbiao; Zhou, Chen; Li, Chang-Jiu; Chen, Liuqing; Zhang, Chunlei; Zhang, Furen; Zheng, Heyi; Zheng, Min; Tu, Ping; Xu, Jinhua; Tao, Juan; Kang, Xiaojing; Zeng, Kang; Lü, Yan; Yu, Nan; Lei, Xia; Pan, Meng; Qiang, Jun; Gu, Heng; Wang, Xiuli

doi:10.1016/j.pdpdt.2021.102340

Cited by 60 publications

(30 citation statements)

References 51 publications

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“…Its interaction of PS, oxygen, and appropriate wavelengths of light in the body tissues leads to the production of reactive oxygen species at the site of photosensitizer accumulation, directly leading to photodamage and consequently to target cell death 21,22 . ALA is a photosensitizer precursor, which is not photosensitizing per se and enters cells to generate protoporphyrin IX through an enzymatic reaction, which accumulates in large quantities in lesioned cells and generates ROS after light irradiation at appropriate wavelengths, causing the death of lesioned cells, damage to blood vessels, and modulation of immune and inflammatory responses 23 . Previous studies have found that PS can selectively accumulate in macrophages that phagocytose pathogenic bacteria or viruses, which can cause macrophage apoptosis and reduce macrophage phagocytic activity 24–27 …”

Section: Discussionmentioning

confidence: 99%

ALA‐PDT regulates macrophage M1 polarization via ERK/MAPK‐NLRP3 pathway to promote the early inflammatory response

et al. 2022

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Background Photodynamic therapy (PDT) is a promising new approach to promote wound healing and its effectiveness has been demonstrated in both clinical and animal studies. Macrophages are the key cells in wound healing and inflammatory response. However, the mechanism of action of PDT on macrophages in promoting wound healing is still unclear. Methods In this study, RAW264.7 cells were used. We analyzed the expression levels of macrophage markers arginase 1 (Arg‐1), CD206, iNOS, CD86, and inflammatory factors IL‐6, TNF‐α, and IL‐1β by reverse transcription‐polymerase chain reaction and Western blot, Milliplex microtubule‐associated protein multiplex assay was performed to analyze the expression of inflammatory factors in the supernatant. Live cell Imaging System to observe the dynamic process of macrophage phagocytosis. Western blot was performed to observe the activation of extracellular signal‐regulated kinase/mitogen‐activated protein kinase (ERK/MAPK) and NOD‐like receptor protein 3 (NLRP3) inflammasome. Results 5‐Aminolevulinic acid (ALA)‐PDT increased the expression of M1 marker iNOS/CD86 and decreased the expression of Arg‐1/CD206 in RAW264.7 cells, while, proinflammatory factors IL‐6, TNF‐α, and IL‐1β expression was enhanced and macrophage phagocytosis was increased. We also found that these phenomena were associated with activation of the ERK/MAPK‐NLRP3 pathway. Conclusion ALA‐PDT promotes early inflammatory responses by regulating macrophage M1 polarization through the ERK/MAPK‐NLRP3 pathway. It also promotes macrophage phagocytosis.

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Section: Discussionmentioning

confidence: 99%

ALA‐PDT regulates macrophage M1 polarization via ERK/MAPK‐NLRP3 pathway to promote the early inflammatory response

et al. 2022

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“…This aspect is fundamental because the action of photodynamic therapy is linked to the perfusion of the photosensitizing agent throughout the canal system, otherwise there can be no bactericidal action by this. In addition, the diffusion of light within the canal system is also necessary; for this reason light sources such as laser diode fibers were used, or fibers were introduced in addition to LED lamps [ 29 , 79 , 80 ].…”

Section: In Vivo Studiesmentioning

confidence: 99%

“…LED lamps also have lower costs than lasers, they are widely used in photodynamic therapy especially for easily accessible tissues, such as in dermatology [ 29 , 79 , 80 ].…”

Section: Introductionmentioning

confidence: 99%

5-Aminolevulinic Acid and Red Led in Endodontics: A Narrative Review and Case Report

D’Ercole

Carlesi

Dotta

et al. 2022

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The present study aims to discuss the main factors involving the use of 5-aminolevulinic acid together with red LED light and its application in endodontic treatment through a narrative review and a case report. Persistence of microorganisms remaining on chemical-mechanical preparation or intracanal dressing is reported as the leading cause of failure in endodontics. Photodynamic therapy has become a promising antimicrobial strategy as an aid to endodontic treatment. Being easy and quick to apply, it can be used both in a single session and in several sessions, as well as not allowing forms of microbial resistance. 5-aminolevulinic acid in combination with red LED light has recently been studied in many branches of medicine, with good results against numerous types of bacteria including Enterococuss faecalis. The case report showed how bacterial count of CFU decreased by half (210 CFU/mL), after 45 min of irrigation with a gel containing 5% of 5-aminolevulinic acid compared to the sample before irrigation (420 CFU/mL). The subsequent irradiation of red LED light for 7 min, the bacterial count was equal to 0. Thus, it is concluded that the use of 5-aminolevulinic acid together with red LED light is effective in endodontic treatment.

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“…Some existing drugs currently used for the treatment of inflammation have been revealed to activate NRF2 and/or upregulate HO-1. For example, 5-aminolevulinic acid (5- ALA) has been widely applied in photodynamic therapy for the treatment of skin diseases and some tumors ( Shi et al, 2021 ). NRF2 activation and HO-1 upregulation by 5-ALA also present therapeutic potential for osteoporosis.…”

Section: Therapeutic Modulation Of Ho-1 In Osteoporosismentioning

confidence: 99%

HO-1 in Bone Biology: Potential Therapeutic Strategies for Osteoporosis

Zhou

Yuan

Xiong

et al. 2021

Front. Cell Dev. Biol.

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Osteoporosis is a prevalent bone disorder characterized by bone mass reduction and deterioration of bone microarchitecture leading to bone fragility and fracture risk. In recent decades, knowledge regarding the etiological mechanisms emphasizes that inflammation, oxidative stress and senescence of bone cells contribute to the development of osteoporosis. Studies have demonstrated that heme oxygenase 1 (HO-1), an inducible enzyme catalyzing heme degradation, exhibits anti-inflammatory, anti-oxidative stress and anti-apoptosis properties. Emerging evidence has revealed that HO-1 is critical in the maintenance of bone homeostasis, making HO-1 a potential target for osteoporosis treatment. In this Review, we aim to provide an introduction to current knowledge of HO-1 biology and its regulation, focusing specifically on its roles in bone homeostasis and osteoporosis. We also examine the potential of HO-1-based pharmacological therapeutics for osteoporosis and issues faced during clinical translation.

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Chinese guidelines on the clinical application of 5-aminolevulinic acid-based photodynamic therapy in dermatology (2021 edition)

Cited by 60 publications

References 51 publications

ALA‐PDT regulates macrophage M1 polarization via ERK/MAPK‐NLRP3 pathway to promote the early inflammatory response

ALA‐PDT regulates macrophage M1 polarization via ERK/MAPK‐NLRP3 pathway to promote the early inflammatory response

5-Aminolevulinic Acid and Red Led in Endodontics: A Narrative Review and Case Report

HO-1 in Bone Biology: Potential Therapeutic Strategies for Osteoporosis

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