HO-1 in Bone Biology: Potential Therapeutic Strategies for Osteoporosis

Zhou, Xueman; Yuan, Wenxiu; Xiong, Xin; Zhang, Zhenzhen; Liu, Jiaqi; Zheng, Yingcheng; Wang, Jun; Liu, Jin

doi:10.3389/fcell.2021.791585

Cited by 24 publications

(13 citation statements)

References 206 publications

(185 reference statements)

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“…This could be explained by the ability of 5-delta aminolevulinic acid to upregulate heme oxygenase 1 (HO-1) as shown in recent studies [33,34]. HO-1 is involved in the normal physiological bone homeostasis, by promoting bone mesenchymal stem cell differentiation, by enhancing osteoblasts functions, and by inhibiting apoptosis and senescence of osteoblasts [35]. In this study, LED irradiation alone did not favor the mineralized nodule formation as well as the 100-ALAD treatment.…”

Section: Discussionmentioning

confidence: 49%

Effect of a 5-aminolevulinic acid gel and 660 nm red LED light on human oral osteoblasts: a preliminary in vitro study

et al. 2022

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This study aimed to evaluate the effects of a new photodynamic protocol (ALAD-PDT) on primary human osteoblasts (hOBs). The ALAD-PDT protocol consists of a heat-sensitive gel with 5% 5-delta aminolevulinic acid commercialized as Aladent (ALAD), combined with 630 nm LED. For this purpose, the hOBs, explanted from human mandible bone fragments, were used and treated with different ALAD concentrations (10%, 50%, 100% v/v) incubated for 45 min and immediately afterwards irradiated with a 630 nm LED device for 7 min. The untreated and unirradiated cells were considered control (CTRL). The cellular accumulation of the photosensitizer protoporphyrin IX (PpIX), the proliferation, the alkaline phosphatase (ALP) activity, and the calcium deposition were assessed. All concentrations (10, 50, 100%) determined a significant increment of PpIX immediately after 45 min of incubation (0 h) with the highest peak by ALAD (100%). The consequent 7 min of light irradiation caused a slight decrease in PpIX. At 48 h and 72 h, any increment of PpIX was observed. The concentration 100% associated with LED significantly increased hOB proliferation at 48 h (+ 46.83%) and 72 h (+ 127.75%). The 50% and 100% concentrations in combination to the red light also stimulated the ALP activity, + 12.910% and + 14.014% respectively. The concentration 100% with and without LED was selected for the assessment of calcium deposition. After LED irradiation, a significant increase in calcium deposition was observed and quantified (+ 72.33%). In conclusion, the ALAD-PDT enhanced proliferation, the ALP activity, and mineralized deposition of human oral osteoblasts, highlighting a promising potential for bone tissue regeneration.

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Section: Discussionmentioning

confidence: 49%

Effect of a 5-aminolevulinic acid gel and 660 nm red LED light on human oral osteoblasts: a preliminary in vitro study

et al. 2022

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“…Withdrawal estrogen increased CCR2, CCL7, and CCL2 expression but reduced PMOP in mice after inhibition of CCL7 and CCL2 synthesis by administration of band [ 39 ]. Hmox1 (HO-1) has been identified as a new potential therapeutic target against OP [ 40 , 41 ]. TBX21 gene is a T-box expressed in T cells.…”

Section: Discussionmentioning

confidence: 99%

[Retracted] The Hub Genes Related to Osteoporosis Were Identified by Bioinformatics Analysis

Ding

Chao

et al. 2023

BioMed Research International

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Osteoporosis (OP) is commonly encountered, which is a kind of systemic injury of bone mass and microstructure, leading to brittle fractures. With the aging of the population, this disease will pose a more serious impact on medical, social, and economic aspects, especially postmenopausal osteoporosis (PMOP). This study is aimed at figuring out potential therapeutic targets and new biomarkers in OP via bioinformatics tools. After differentially expressed gene (DEG) analysis, we successfully identified 97 upregulated and 172 downregulated DEGs. They are mainly concentrated in actin binding, regulation of cytokine production, muscle cell promotion, chemokine signaling pathway, and cytokine-cytokine receiver interaction. According to the diagram of protein-protein interaction (PPI), we obtained 10 hub genes: CCL5, CXCL10, EGFR, HMOX1, IL12B, CCL7, TBX21, XCL1, PGR, and ITGA1. Expression analysis showed that Egfr, Hmox1, and Pgr were significantly upregulated in estrogen-treated osteoporotic patients, while Ccl5, Cxcl10, Il12b, Ccl7, Tbx21, Xcl1, and Itga1 were significantly downregulated. In addition, the analysis results of Pearson’s correlation revealed that CCL7 has a strong positive association with IL12b, TBX21, and CCL5 and so was CCL5 with IL12b. Conversely, EGFR has a strong negative association with XCL1 and CXCL10. In conclusion, this study screened 10 hub genes related to OP based on the GEO database, laying a biological foundation for further research on new biomarkers and potential therapeutic targets in OP.

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“…Much of its effect is linked with the released CO that has widespread interactions with different cells and cell functions. Both HO-1 and HO-2 are identified as therapeutic targets in widely different diseases [ 147 , 148 , 149 , 150 , 151 ]. CO and carbon-monoxide-releasing molecules (CORM) are also investigated as therapeutic potentials [ 152 , 153 ].…”

Section: Exhaled Gaso-transmittersmentioning

confidence: 99%

Exhaled Biomarkers for Point-of-Care Diagnosis: Recent Advances and New Challenges in Breathomics

et al. 2023

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Cancers, chronic diseases and respiratory infections are major causes of mortality and present diagnostic and therapeutic challenges for health care. There is an unmet medical need for non-invasive, easy-to-use biomarkers for the early diagnosis, phenotyping, predicting and monitoring of the therapeutic responses of these disorders. Exhaled breath sampling is an attractive choice that has gained attention in recent years. Exhaled nitric oxide measurement used as a predictive biomarker of the response to anti-eosinophil therapy in severe asthma has paved the way for other exhaled breath biomarkers. Advances in laser and nanosensor technologies and spectrometry together with widespread use of algorithms and artificial intelligence have facilitated research on volatile organic compounds and artificial olfaction systems to develop new exhaled biomarkers. We aim to provide an overview of the recent advances in and challenges of exhaled biomarker measurements with an emphasis on the applicability of their measurement as a non-invasive, point-of-care diagnostic and monitoring tool.

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HO-1 in Bone Biology: Potential Therapeutic Strategies for Osteoporosis

Cited by 24 publications

References 206 publications

Effect of a 5-aminolevulinic acid gel and 660 nm red LED light on human oral osteoblasts: a preliminary in vitro study

Effect of a 5-aminolevulinic acid gel and 660 nm red LED light on human oral osteoblasts: a preliminary in vitro study

[Retracted] The Hub Genes Related to Osteoporosis Were Identified by Bioinformatics Analysis

Exhaled Biomarkers for Point-of-Care Diagnosis: Recent Advances and New Challenges in Breathomics

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