Chemotactic selection of frozen-thawed stallion sperm improves sperm quality and heterologous binding to oocytes

Domïnguez, E.; Moreno-Irusta, Ayelén; Rodríguez, María Belén; Salamone, D.; Arruda, Rubens Paes de; Losinno, Luis; Giojalas, Laura Cecilia

doi:10.1016/j.anireprosci.2020.106582

Cited by 4 publications

(2 citation statements)

References 67 publications

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“…Further evidence of sperm selection based on their ability to respond to chemotaxis comes from IVF studies that have tested different chemotaxis chambers for sperm selection. Overall, they showed that sperm that are chemo-attracted to either progesterone or FF have higher DNA integrity and achieved higher fertilization rates compared with unselected sperm [129][130][131][132]. Yet, these studies do not represent physiological conditions and do not compare results with non-chemo-responsive sperm after being exposed to the same chambers.…”

Section: Bull Oec Suspensionmentioning

confidence: 99%

Sperm selection by the oviduct: perspectives for male fertility and assisted reproductive technologies

Soto-Heras

Sakkas

Miller

2023

Biology of Reproduction

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The contribution of sperm to embryogenesis is gaining attention with up to 50% of infertility cases being attributed to a paternal factor. The traditional methods used in assisted reproductive technologies (ART) for selecting and assessing sperm quality are mainly based on motility and viability parameters. However, other sperm characteristics, including DNA integrity, have major consequences for successful live birth. In natural reproduction, sperm navigate the male and female reproductive tract to reach and fertilize the egg. During transport, sperm encounter many obstacles that dramatically reduce the number arriving at the fertilization site. In humans, the number of sperm is reduced from tens of millions in the ejaculate to hundreds in the Fallopian tube (oviduct). Whether this sperm population has higher fertilization potential is not fully understood, but several studies in animals indicate that many defective sperm do not advance to the site of fertilization. Moreover, the oviduct plays a key role in fertility by modulating sperm transport, viability, and maturation, providing sperm that are ready to fertilize at the appropriate time. Here we present evidence of sperm selection by the oviduct with emphasis on the mechanisms of selection and the sperm characteristics selected. Considering the sperm parameters that are essential for healthy embryonic development, we discuss the use of novel in vitro sperm selection methods that mimic physiological conditions. We propose that insight gained from understanding how the oviduct selects sperm can be translated to ART to yield high fertilization, embryonic development, and pregnancy rates.

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Section: Bull Oec Suspensionmentioning

confidence: 99%

Sperm selection by the oviduct: perspectives for male fertility and assisted reproductive technologies

Soto-Heras

Sakkas

Miller

2023

Biology of Reproduction

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“…[7][8][9] Because progesterone can stimulate capacitation in some spermatozoa, exposure to progesterone has been used as a chemokine to prepare the best spermatozoa. 7,8,10 Progesterone regulates sperm function through a variety of signal pathways, including phosphorylation of heat shock protein 90 11 and Ca 2þ cation channels (CatSper). 12,13 The CatSper-Ca 2þ signalling pathway is very important for the regulation of human sperm function.…”

Section: Introductionmentioning

confidence: 99%

Progesterone activates the cyclic AMP-protein kinase A signalling pathway by upregulating ABHD2 in fertile men

et al. 2021

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Objective This was a prospective study to investigate whether progesterone affects sperm activity by regulating the cyclic AMP-protein kinase A (cAMP-PKA) signalling pathway via α/β hydrolase domain-containing protein 2 (ABHD2). Methods Spermatozoa were collected from healthy and infertile men (with oligoasthenospermia or abnormal acrosome; n = 30/group). The expression of and mutations in ABHD2 were detected by quantitative PCR, western blot, and gene sequencing. The expression of ABHD2 in the presence of progesterone was detected in all groups, and cAMP and PKA levels were detected by ELISA in fertile men after treatment with ABHD2 antibody and PKA inhibitor H-89, respectively. Results Expression of ABHD2 mRNA and protein were reduced in spermatozoa from infertile compared with fertile men. Four gene mutation sites were detected in spermatozoa from the infertile groups. Progesterone increased mRNA and protein levels of ABHD2 in healthy spermatozoa but not in spermatozoa from infertile men. The levels of cAMP and PKA were increased by progesterone in healthy spermatozoa, and the progesterone-increased cAMP and PKA were decreased by ABHD2 antibody and H-89, respectively. Conclusion Progesterone regulates the ABHD2-mediated cAMP-PKA signalling pathway in healthy spermatozoa, which provides a new target for clinical diagnosis and treatment of infertility.

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The stallion sperm acrosome: Considerations from a research and clinical perspective

et al. 2023

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Chemotactic selection of frozen-thawed stallion sperm improves sperm quality and heterologous binding to oocytes

Cited by 4 publications

References 67 publications

Sperm selection by the oviduct: perspectives for male fertility and assisted reproductive technologies

Sperm selection by the oviduct: perspectives for male fertility and assisted reproductive technologies

Progesterone activates the cyclic AMP-protein kinase A signalling pathway by upregulating ABHD2 in fertile men

The stallion sperm acrosome: Considerations from a research and clinical perspective

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