The stallion sperm acrosome: Considerations from a research and clinical perspective

Hernández‐Avilés, Camilo; Ramírez‐Agámez, Luisa; Varner, D.D.; Love, Charles C.

doi:10.1016/j.theriogenology.2022.11.012

Cited by 2 publications

(1 citation statement)

References 503 publications

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“…Semen collection from breeding inactive stallions is also performed at 1 h apart for breeding soundness evaluation and semen cooling and freezing tests [ 28 , 29 ]. However, scant evidence supports or refutes the use of the first ejaculate of the reproductive season for semen cooling and freezing testing.…”

Section: Discussionmentioning

confidence: 99%

Semen Quality of the First and Second Ejaculates Collected from Breeding Inactive Stallions after Cooling and Freezing

Podico

Spencer

Magalhaes

et al. 2023

Veterinary Sciences

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This study aimed to assess the semen quality after the cooling and freezing of the first and second ejaculates of the season, which were collected 1 h apart. After collection (n = 40 ejaculates), the gel-free semen volume, concentration, total number of sperm, and sperm morphology were determined. An aliquot of each ejaculate was extended and cooled for 48 h; a second aliquot was cushion-centrifuged and cooled for 48 h; and a third aliquot was processed and then frozen. The total motility (TM) and progressive motility (PM), plasma membrane integrity (PMI), and high mitochondrial membrane potential (HMMP) were assessed pre-(0 h), 24 h, and 48 h post-cooling and before and after freezing. The second ejaculate had a lower gel-free semen volume (p = 0.026). The sperm concentration was greater in the first than in the second ejaculate (p < 0.001). The sperm morphology was similar between the ejaculates (p > 0.05). Cushion-centrifugation prevented a reduction in the TM, PM, and PMI over time (p < 0.05). The TM, PM, and PMI decreased after freezing but not between the ejaculates (p > 0.05). The first and second ejaculates of the season, which were collected 1 h apart, varied in quantity but not in quality after cooling and freezing.

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Section: Discussionmentioning

confidence: 99%

Semen Quality of the First and Second Ejaculates Collected from Breeding Inactive Stallions after Cooling and Freezing

Podico

Spencer

Magalhaes

et al. 2023

Veterinary Sciences

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Proteomic analysis of sperm from fertile stallions and subfertile stallions due to impaired acrosomal exocytosis

Hernández-Avilés,

Ramírez-Agámez,

Weintraub

et al. 2024

Preprint

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Thoroughbred stallions that carry a double-homozygous genotype A/A-A/A for SNPs rs397316122 and rs69101140 in exon 5 of the FKBP6 gene (chr13; EquCab3.0) are uniquely subfertile due to impaired acrosomal exocytosis (IAE). In this study, the sperm proteome in frozen/thawed semen from subfertile Thoroughbred stallions was studied and compared to that of frozen/thawed sperm from fertile Thoroughbred stallions. A total of 2,220 proteins was identified, of which 140 proteins were found to be differentially abundant in sperm from the subfertile stallions when compared to that of fertile stallions (83 less and 57 more abundant). Proteins of differential abundance in sperm from the subfertile stallions were mostly overrepresented in the “metabolism” and the “metabolism of lipids” pathways. One of these proteins, arylsulfatase F (ARSF), was studied by immunofluorescence. A lower proportion of sperm displaying ARSF signal at the acrosome region was observed in sperm from subfertile Thoroughbred stallions. In addition, heterologous zona pellucida binding assays were performed and revealed sperm from subfertile Thoroughbred stallions bound at a lower proportion to zonae pellucidae than sperm from fertile Thoroughbred stallions. In conclusion, a group of proteins of differential abundance, including some of acrosome origin, were identified in sperm from subfertile stallions with acrosome dysfunction.

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The stallion sperm acrosome: Considerations from a research and clinical perspective

Cited by 2 publications

References 503 publications

Semen Quality of the First and Second Ejaculates Collected from Breeding Inactive Stallions after Cooling and Freezing

Semen Quality of the First and Second Ejaculates Collected from Breeding Inactive Stallions after Cooling and Freezing

Proteomic analysis of sperm from fertile stallions and subfertile stallions due to impaired acrosomal exocytosis

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