Chemometric evaluations of repeatability and detection limit in high-performance liquid chromatography with electrochemical detection

Kotani, Akio; Watanabe, Ryo; Hayashi, Yuzuru; Hakamata, Hideki

doi:10.1016/j.chroma.2022.463075

Cited by 5 publications

(1 citation statement)

References 33 publications

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“…The repeatability and intermediate precision as parameters were considered to access the suitability of the developed extraction method and the applicability in pine bark residues [ 43 ]. Six extractions were carried out under optimal conditions on the same day for the repeatability assay whereas 6 more extractions were performed on 2 consecutive days for the intermediate precision analysis, resulting in a total of 18 extractions.…”

Section: Resultsmentioning

confidence: 99%

Ultrasound-Assisted Extraction of Polyphenols from Maritime Pine Residues with Deep Eutectic Solvents

Duarte

Gomes

Aliaño-González

et al. 2022

Foods

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Deep eutectic solvents represent an important alternative in the field of green solvents due to their low volatility, non-toxicity, and low synthesis cost. In the present investigation, we propose the production of enriched polyphenolic extracts from maritime pine forest residues via an ultrasound-assisted approach. A Box–Behnken experimental design with a response surface methodology was used with six variables to be optimized: solid-to-solvent ratio, water percentage, temperature and time of extraction, amplitude, and catalyst concentration. The mixture of levulinic and formic acids achieved the highest extraction yield of polyphenols from pine needle and bark biomass. In addition, the solid-to-solvent ratio was found to be the only influential variable in the extraction (p-value: 0.0000). The optimal conditions were established as: 0.1 g of sample in 10 mL of LA:FA (70:30%, v/v) with 0% water and 0 M H2SO4 heated to 30 °C and extracted during 40 min with an ultrasound amplitude of 80% at 37 kHz. The bioactive properties of polyphenol-enriched extracts have been proven with significant antioxidant (45.90 ± 2.10 and 66.96 ± 2.75 mg Trolox equivalents/g dw) and antimicrobial activities. The possibility to recycle and reuse the solvent was also demonstrated; levulinic acid was successfully recovered from the extracts and reused in novel extractions on pine residues. This research shows an important alternative to obtaining polyphenol-enriched extracts from forest residues that are commonly discarded without any clear application, thus opening an important window toward the valorization of such residues.

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Section: Resultsmentioning

confidence: 99%

Ultrasound-Assisted Extraction of Polyphenols from Maritime Pine Residues with Deep Eutectic Solvents

Duarte

Gomes

Aliaño-González

et al. 2022

Foods

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Effects of digital processing on repeatability assessment of a multiple reaction monitoring liquid chromatography–tandem mass spectrometry system by ISO 11843‐7

Machida

Kotani

Hayashi³

et al. 2022

J Mass Spectrom

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ISO 11843 part 7 (ISO 11843-7) can provide a standard deviation (SD) of area measurements of a target peak through the stochastic behaviors of instrumental noises. The purpose of this study is to demonstrate that ISO 11843-7 can be applied to assess repeatability in an isocratic liquid chromatography-tandem mass spectrometry (LC-MS/MS) system without repetitive measurements. The relative standard deviation (RSD) of the peak area of ergosterol picolinyl ester, which was used as an example, on a multiple reaction monitoring (MRM) chromatogram was determined by ISO 11843-7. The RSD by ISO 11843-7 (N = 1) was within a 95% confidence band of the RSD by repetitive measurements (N = 6). Moreover, the effects of digital smoothing, such as moving average, were also examined on the repeatability assessment in LC-MS/MS by ISO 11843-7. From the results of the comparisons of the RSDs obtained by ISO 11843-7 and the repetitive measurements, it was shown that suitable RSDs of the peak area were obtained from the smoothed MRM chromatograms by the moving average for narrow data point windows (e.g., one-sixth of the peak width). In conclusion, the utility of repeatability assessment based on ISO 11843-7 has been expanded for the validation of an LC-MS/MS system.

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Optimization of the extraction of polyphenols from Pinus pinaster residues using deep eutectic solvents: a sustainable approach

Duarte,

Gomes,

Aliaño-González

et al. 2023

Wood Sci Technol

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Chemometric evaluations of repeatability and detection limit in high-performance liquid chromatography with electrochemical detection

Cited by 5 publications

References 33 publications

Ultrasound-Assisted Extraction of Polyphenols from Maritime Pine Residues with Deep Eutectic Solvents

Ultrasound-Assisted Extraction of Polyphenols from Maritime Pine Residues with Deep Eutectic Solvents

Effects of digital processing on repeatability assessment of a multiple reaction monitoring liquid chromatography–tandem mass spectrometry system by ISO 11843‐7

Optimization of the extraction of polyphenols from Pinus pinaster residues using deep eutectic solvents: a sustainable approach

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