Chemometric Evaluations of Repeatability and Detection Limit in High-Performance Liquid Chromatography with Electrochemical Detection

Effects of digital processing on repeatability assessment of a multiple reaction monitoring liquid chromatography–tandem mass spectrometry system by ISO 11843‐7

“…In a previous study, we showed that ISO 11843‐7 has been applied to repeatability assessments in gas chromatography–mass spectrometry (GC–MS), high‐performance liquid chromatography with UV detection (HPLC‐UV), electrochemical detection (HPLC‐ECD), and refractive index detection (HPLC‐RI) 17–19,21–23 . It has yet to be demonstrated whether ISO 11843‐7 can be applied to assess repeatability in LC–MS/MS and determine an RSD of a peak area on an MRM chromatogram.…”

“…[11][12][13][14][15][16][17][18][19] The Function of Mutual Information (FUMI) theory is one of the chemometric tools based on a stochastic theory. [17][18][19] The FUMI theory predicts the standard deviation (SD) of area measurements of a target peak from the stochastic behaviors of baseline noise in an instrumental analysis without repetitive measurements. The FUMI theory has been adopted into ISO 11843 part 7 (ISO 11843-7:2018, "Methodology based on stochastic properties of instrumental noise"), and the SD of area measurements obtained by the FUMI theory has been provided to determine detection limits in instrument analyses.…”

“…In a previous study, we showed that ISO 11843-7 has been applied to repeatability assessments in gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography with UV detection (HPLC-UV), electrochemical detection (HPLC-ECD), and refractive index detection (HPLC-RI). [17][18][19][21][22][23] It has yet to be demonstrated whether ISO 11843-7 can be applied to assess repeatability in LC-MS/MS and determine an RSD of a peak area on an MRM chromatogram. If repeatability assessments for MRM chromatograms can be provided by ISO 11843-7, experimental time, effort, and chemical amounts required would be saved in the validation of an LC-MS/MS system.…”

“…To assess repeatability in an HPLC system, a relative standard deviation (RSD) of an area of a target peak is usually determined by repetitive measurements ( N = 5 or 6), 10 whereas some chemometric tools based on the idea that the major source of uncertainty is attributed to the instrumental noises caused by apparatuses have also been proposed to determine an RSD 11–19 . The Function of Mutual Information (FUMI) theory is one of the chemometric tools based on a stochastic theory 17–19 .…”

“…To assess repeatability in an HPLC system, a relative standard deviation (RSD) of an area of a target peak is usually determined by repetitive measurements (N = 5 or 6), 10 whereas some chemometric tools based on the idea that the major source of uncertainty is attributed to the instrumental noises caused by apparatuses have also been proposed to determine an RSD. [11][12][13][14][15][16][17][18][19] The Function of Mutual Information (FUMI) theory is one of the chemometric tools based on a stochastic theory. [17][18][19] The FUMI theory predicts the standard deviation (SD) of area measurements of a target peak from the stochastic behaviors of baseline noise in an instrumental analysis without repetitive measurements.…”