Chemiluminescent (CL) functionalized
nanomaterials with good CL
property, excellent catalytic performance, and fast magnetic separation
ability have great help in CL biological analysis. In this study,
the magnetic core cobalt ferrite nanoparticles (CoFe2O4 NPs), possessing good peroxidase (POD)-like activity, were
synthesized directly by a one-step solvothermal process. Then, N-(4-aminobutyl)-N-ethylisoluminol-gold
NPs (ABEI-Au NPs) were modified on the CoFe2O4 NPs surface via a redox method to obtain CoFe2O4@ABEI-Au NPs. The synthetic NPs exhibited a spherical structure featuring
a mean size of 125 ± 20 nm. The CL property of CoFe2O4@ABEI-Au NPs was more than 100 times that of ABEI-Au
NPs, showing the good catalytic property of CoFe2O4 NPs. Furthermore, CoFe2O4@ABEI-Au NPs
also displayed good superparamagnetism, which could be quickly separated
from the mixture. Hence, a label-free CL immunosensor was established
on the basis of CoFe2O4@ABEI-Au NPs for copeptin
detection. The immunosensor had a wide linear range from 1.0 ×
10–13 to 1.0 × 10–8 g/mL
and a low detection limit of 3.3 × 10–14 g/mL,
which is better than most of the immunosensors reported so far. The
developed immunosensor was used for the analysis of copeptin in human
serum specimens, and the measured results were close to the values
of enzyme linked immunosorbent assay (ELISA), manifesting the feasibility
in AMI clinical diagnosis. This label-free CL immunosensor featuring
ease of fabrication, sensitivity, and rapid separation provides a
versatile platform for bioanalysis.