Au Nanoparticle-Decorated Magnetic CoFe₂O₄ Core Nanoparticles Functionalized with N-(4-Aminobutyl)-N-ethylisoluminol for Detection of Copeptin

Abstract

Chemiluminescent (CL) functionalized nanomaterials with good CL property, excellent catalytic performance, and fast magnetic separation ability have great help in CL biological analysis. In this study, the magnetic core cobalt ferrite nanoparticles (CoFe2O4 NPs), possessing good peroxidase (POD)-like activity, were synthesized directly by a one-step solvothermal process. Then, N-(4-aminobutyl)-N-ethylisoluminol-gold NPs (ABEI-Au NPs) were modified on the CoFe2O4 NPs surface via a redox method to obtain CoFe2O4@ABEI-Au NPs. The synthetic NPs exhibited a spherical structure featuring a mean size of 125 ± 20 nm. The CL property of CoFe2O4@ABEI-Au NPs was more than 100 times that of ABEI-Au NPs, showing the good catalytic property of CoFe2O4 NPs. Furthermore, CoFe2O4@ABEI-Au NPs also displayed good superparamagnetism, which could be quickly separated from the mixture. Hence, a label-free CL immunosensor was established on the basis of CoFe2O4@ABEI-Au NPs for copeptin detection. The immunosensor had a wide linear range from 1.0 × 10–13 to 1.0 × 10–8 g/mL and a low detection limit of 3.3 × 10–14 g/mL, which is better than most of the immunosensors reported so far. The developed immunosensor was used for the analysis of copeptin in human serum specimens, and the measured results were close to the values of enzyme linked immunosorbent assay (ELISA), manifesting the feasibility in AMI clinical diagnosis. This label-free CL immunosensor featuring ease of fabrication, sensitivity, and rapid separation provides a versatile platform for bioanalysis.

N-(4-Aminobutyl)-N-ethylisopropanol and Co2+ Dual-Functionalized Core–Shell Fe3O4@Au/Ag Magnetic Nanomaterials with Strong and Stable Chemiluminescence for a Label-Free Exosome Immunosensor

“…A new sensing principle is proposed. The immunosensor exhibited excellent analytical performance with a wide detection range of 3.1 × 10 to 3.1 × 10 8 particles/mL and a low detection limit of 2.1 × 10 3 particles/mL, which were better than the vast majority of the reported CL immunosensors. Moreover, the as-developed CL immunosensor is based on a label-free strategy that does not require complex labeling procedures.…”

“…2 Yang et al synthesized ABEI and gold nanoparticle (AuNP)-functionalized magneticcore cobalt ferrite nanomaterials and used them to develop a label-free CL immunosensor for copeptin detection. 3 Although these label-free strategies based on CL-functionalized MBs have achieved good performance, there are still same drawbacks that need to be improved. It is known that MBs have a non-negligible quenching effect on CL emission, thereby reducing the CL intensity of functionalized materials and affecting the sensitivity of biosensors.…”

“…For example, our group synthesized N -(4-aminobutyl)- N -ethylisopropanol (ABEI) and Co 2+ -functionalized MBs (Co 2+ /ABEI/MBs) with a good CL property, which were used to develop a label-free biosensor for the determination of 2,4,6-trinitrotoluene . Yang et al synthesized ABEI and gold nanoparticle (AuNP)-functionalized magnetic-core cobalt ferrite nanomaterials and used them to develop a label-free CL immunosensor for copeptin detection . Although these label-free strategies based on CL-functionalized MBs have achieved good performance, there are still same drawbacks that need to be improved.…”

Bimetallic CoMoO4 Nanozymes Enhanced Luminol Chemiluminescence for the Detection of Dopamine

“…Nanozymes are of interest because of their high stability and catalytic activity compared to natural enzymes . Transition metal-based nanozymes (TMNs) have gained prominence owing to their environmental friendliness, low cost, outstanding catalytic properties, and ease of synthesis. , Numerous TMNs exhibiting oxidase or peroxidase activity have been synthesized to improve CL efficiency for highly sensitive detection of analytes. , For example, Yang et al synthesized CoFe 2 O 4 with peroxidase-like activity and achieved sensitive detection of copeptin; Yang et al reported CoOOH nanoflakes that exhibited remarkable oxidase-mimicking activity to enhance the luminol CL . Multimetallic materials may exhibit superior catalytic properties compared to their monometallic counterparts because of the synergistic effects of center metals .…”

“…25,26 Numerous TMNs exhibiting oxidase or peroxidase activity have been synthesized to improve CL efficiency for highly sensitive detection of analytes. 27,28 For example, Yang et al synthesized CoFe 2 O 4 with peroxidase-like activity and achieved sensitive detection of copeptin; 29 Yang et al reported CoOOH nanoflakes that exhibited remarkable oxidasemimicking activity to enhance the luminol CL. 30 Multimetallic materials may exhibit superior catalytic properties compared to their monometallic counterparts because of the synergistic effects of center metals.…”