Chemically modified proteins solubilized in AOT reverse micelles. Influence of proteins charges on intermicellar interactions

Impact of Surface Active Compounds on Iron Catalyzed Oxidation of Methyl Linolenate in AOT–Water–Hexadecane Systems

“…Samples containing hexadecane, AOT (100 mmol/kg lipid), iron (1 μmol/kg lipid), and methyl linolenate (2% w/w) had a scattering intensity appreciably higher than samples containing hexadecane alone ( Figure 3). The scattering intensity profiles of the AOT, iron, and methyl linolenate samples fit closely to the spherical form factor 14 suggesting that AOT formed monodispersed spherical reverse micelles upon addition to hexadecane, which is in agreement with earlier findings with AOT in various nonpolar solvents. [19][20][21][22] Multivalent transition metals such as iron and copper are effective lipid oxidation catalysts.…”

The influence of structure and composition of a reverse SDS microemulsion on enzymatic activities and electrical conductivities

“…As a consequence, the enzyme should not significantly influence the structure of the medium, although the enzyme is probably larger than the expected size of the microemulsion droplets. This is in contrast to other studies, e.g., those carried out in Pileni's group [66,67] where a protein such as cytochrome C was able to reorganize the reverse micelles in percolating systems. As the HRP has a similar structure and hydropathy as cytochrome C, a certain adaptation of the reverse micelles around a HRP molecule cannot be completely excluded.…”

Structure of AOT reverse micelles under shear

“…At low water contents, AOT reverse micelles are commonly described using a model of spherical "core and shell" aggregates [22]. This model describes an aggregate with an outer diameter of R s , built on a core having a radius of R c and electron density of ρ c , surrounded by a thin spherical layer having an electron density of ρ s .…”