The influence of structure and composition of a reverse SDS microemulsion on enzymatic activities and electrical conductivities

Oxidation Catalysis by Enzymes in Microemulsions

“…This was attributed to the solubility of each alcohol in the buffer. The study underlies the importance of a quick thermodynamic equilibrium for the microemulsion because in the opposite case (as happens with the addition of long-chain alcohols) the enzyme is exposed to denaturation factors [43]. The above conclusion supports the fact that enzymatic activity in microemulsions based on SDS is lower than in systems based on the more hydrophobic AOT, where the equilibrium is immediately reached.…”

“…It is generally observed, that in microemulsions with SDS as surfactant, increased concentration of this component leads to enhanced activity of HRP. The above observation can be explained by the fact that SDS leads to a decrease in the buffer pH that is favorable for the enzyme [43]. Parida et al [44] enhanced the catalytic activity of the enzyme at higher temperatures and pH values with the addition of cholesterol molecules which interacted with the head group of AOT in an isooctane/AOT/cholesterol/water system and hardened the membrane (substrates: o-phenylenediamine, pyrogallol).…”

“…Bauduin et al [43] demonstrated that for wo values of approximately 20, the system dodecane/SDS/n-alcohol/water (with different alcohols as co-surfactants) offers a properly hydrated …”

“…Bauduin et al [43] demonstrated that for w o values of approximately 20, the system dodecane/ SDS/n-alcohol/water (with different alcohols as co-surfactants) offers a properly hydrated environment for the enzyme and as a result its maximum activity occurs when tested towards the oxidation of ABTS. In the same study, it was shown that when using 1-butanol and 1-heptanol, enzymatic activity presents two different linear parts when plotted versus w o , with a sharp increase in the first part and a slight decrease in the second part, whereas, when 1-hexanol and 1-pentanol were used, the plot exhibits three distinctive regions.…”

Formation of W/O Microemulsion Based on Natural Glycolipid Biosurfactant, Mannosylerythritol Lipid-A

“…Moreover, glycolipid biosurfactant-based microemulsion has a great advantage not only for environmental but also for biological applications including protein crystallization and separation matrix 21) , or enzymatic reaction media 22,23) . This is because the sugar moiety of MEL-A was previously found to give an antimicrobial activity as well as a high affinity toward various proteins such as immunoglobulins 24) and lectins 25) .…”