Structure of AOT reverse micelles under shear

Nanoencapsulated Lecitase Ultra and Thermomyces lanuginosus Lipase, a Comparative Structural Study

“…As shown in Figure 3, for both enzymes the size of the reverse micelles increased with temperature. This phenomenon could be related to the formation of cylindrical structures in accordance to the results discussed above 26 . The importance of this event is directly related to 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 18 mass transfer, which could be favored promoting biocatalytic reactions more efficiently.…”

“…This was observed by DLS at different w 0 values. Some authors 23,25,26 already reported increases in the attractive forces between the droplets with the increment of temperature. In these cases, there are most enduring collisions.…”

Structure modifications of AOT reverse micelles due to protein incorporation

“…Indeed, a good fit to the experimental data was obtained by suggesting a two-component model that takes into account the coexistence of small spherical micelles having a diameter of 1 nm (solid line in Fig. 9) [26]. The ratio of surfactant molecules forming cylindrical micelles to those forming spherical micelles, N c /N s , which was calculated from the fit assuming an identical area per headgroup in both types of aggregates was found to be equal to 223.…”

“…BSA (MW 66 kDa, pI 4.9) is a bulky hydrophilic protein having radius of about 3.5 nm [20,21], while lysozyme (M w 14 kDa, pI 11.1) has a much smaller radius of about 2 nm. To allow us to examine different scenarios, we chose to work at two water contents: W 0 = 40, for which the average reverse micelle size (4.9 nm [26]) is larger than both proteins, and W 0 = 20, where the reverse micelles (2.5 nm) could be large enough to Notes. C lys : concentration of solubilized lysozyme in reverse micelles solution; W 0 : molar ratio of water to surfactant; n p : number of micelles per unit volume; R c : mean radius of the water core; σ : root-mean-square deviation from the mean water core radius.…”

Effect of counterion substitution on the viscosity anomaly in AOT microemulsions

“…For the sample compositions that we examine ( X < 25 and Φ 0.2) we do not expect any non-Newtonian effects. Detailed rheological experiments of AOT/water/isooctane microemulsions suggest that shear-thinning effects are only observed at for samples with much higher water content, X > 70 [20]. Thus, for analysis of our data, we assume that the viscosities obtained from capillary viscometry are equivalent to the low-shear viscosity and that they can be analyzed using Eqs.…”