Chemical Radiosensitization of Anoxic Mammalian Cells: Effect of Cell Concentration

Agnew, D. A.; Skarsgard, L. D.

doi:10.2307/3573831

Cited by 19 publications

(6 citation statements)

References 10 publications

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“…The finding that TAN decreases toxicity in dilute suspension suggests a search for a compound which would have this effect in cell pellets and in vivo, since TAN itself, unfortunately, appears to be inactivated in pellets (this work, Agnew and Skarsgard 1974) and in blood (Blackett et al . Whereas its accompanying toxicity to hypoxic cells may be advantageous, it appears that it may limit the clinical application of Miso, due to detrimental effects on normal hypoxic tissues .…”

Section: 2 Implicationsmentioning

confidence: 83%

“…The cells used in this study were of the Chinese hamster line CH2B 2 (Agnew and Skarsgard 1972), grown in plastic flasks (Falcon Plastics) at 37°C under a CO 2 (5 per cent)/air (95 per cent) atmosphere . The culture medium was MEM with Hanks balanced salts (Grand Island Biological Co .…”

Section: Materials and Methods 21 Cellsmentioning

confidence: 99%

“…Details of experimental procedures have been previously described (Agnew andSkarsgard 1974, Moore, Palcic andSkarsgard 1976) . The dilute suspensions were stirred, and maintained under an atmosphere of nitrogen (02 less than 5 p.p.m.)…”

Section: Materials and Methods 21 Cellsmentioning

confidence: 99%

“…Figure 6 shows the effect of varying TAN concentration at constant (15 mM) Miso concentration . Agnew and Skarsgard (1974) have shown that TAN is not effective as a sensitizer of hypoxic cells in a pellet, which they proposed as a model for studying sensitization of tissue . The protective effect of TAN increases with concentration until the toxicity of TAN itself becomes a factor .…”

Section: Int J Radiat Biol Downloaded From Informahealthcarecom By Mmentioning

confidence: 99%

“…The presence of TAN at a concentration as low as 1 mM has a marked effect ; this curve is comparable to approximately 5 mM Miso alone . Lack of sensitization by nitroxyls in tissues or pellet may be due to reduction of the free radical, enzymatic degradation, or some inactivation due to binding (Millar, Fielden and Smithen 1977, Blackett, Wooliscroft, Fielden and Skov et al Lillicrap 1974, Agnew andSkarsgard 1974) . Figure 6 shows that 50 mM TAN, with or without Miso, is very toxic .…”

Section: Int J Radiat Biol Downloaded From Informahealthcarecom By Mmentioning

confidence: 99%

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Modification of the Action of Misonidazole I. Effect of TAN on Toxicity

Skov¹,

Palcic²,

Harrison³

et al. 1980

International Journal of Radiation Biology and Related Studies

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The toxicity of misonidazole, an electron affinic radiosensitizer, is greatly reduced by TAN, a free radical radiosensitizer. The production of single-strand breaks in DNA of mammalian cells incubated in dilute suspension with misonidazole (15 mM) under hypoxic conditions is greatly decreased by the presence of TAN (10 mM). The survival of such cells is also greatly enhanced if TAN is present at a concentration of 10mM even less. The implications and possible mechanisms of this finding are discussed.

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Section: 2 Implicationsmentioning

confidence: 83%

Section: Materials and Methods 21 Cellsmentioning

confidence: 99%

Section: Materials and Methods 21 Cellsmentioning

confidence: 99%

Section: Int J Radiat Biol Downloaded From Informahealthcarecom By Mmentioning

confidence: 99%

Section: Int J Radiat Biol Downloaded From Informahealthcarecom By Mmentioning

confidence: 99%

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