Chemical profiling, HPLC characterization and in-vitro antioxidant potential of Pakistani propolis collected from peripheral region of Faisalabad

Abstract

Propolis is a highly adhesive and resinous product of honey bee (Apis mellifera L.) which is produced from the exudations of plants. Bee propolis being a source of bioactive compounds like polyphenols and flavonoids imparts numerous biological properties including, antioxidant, anti-inflammatory, antimicrobial and anticancer activities. Present study was designed to elucidate the composition and antioxidant status of locally available propolis using in-vitro conditions. Propolis collected from locally found apiaries and its hydroalcoholic extract of propolis was prepared using different concentrations of ethanol and methanol. The results regarding proximate composition of propolis showed a higher proportion of ether extract (85.59±0.87%) and lowest contents of crude fiber (0.31±0.08%). Among the mineral's sodium, potassium and calcium was found in a concentration of 11.33±0.91, 52.10±2.9 and 10.53±0.83.59±0.23mg/Kg respectively whilst zinc was noticed as 3.59±0.23mg/Kg. HPLC characterization indicates a highest concentration of Chlorogenic acid 31.80±2.56mg/Kg whereas gallic acid (0.21±0.01mg/Kg) was found in lowest concentration among the polyphenols. Ethanol extract represents more phenolic contents, DPPH activity and antioxidant status as 327.30±14.89mg/gGAE, 73.18±4.43% and 60.59±4.38% accordingly in comparison to methanol and water extract. Bee propolis found an effective source of natural antioxidants which retards the production of free radicals and reactive oxygen species thus help to cope oxidative stress.

Bio-Functional Potential and Biochemical Properties of Propolis Collected from Different Regions of Balochistan Province of Pakistan

“…According to Zehra, Yildiz, Şahin, Asadov, and Kolayli [23], the antioxidant potential of propolis extracts have a positive correlation with their phenolic, flavonoids, and other bioactive contents. Recentlym Shahbaz et al [1] reported the DPPH free radical scavenging activity of propolis up to 70% which is in accord to the findings of current study. In a similar way Choi et al [20] concluded that propolis extract from Korea exhibits higher antioxidant activity as compared to Brazilian propolis due to higher concentration of flavonoids and phenolic contents.…”

“…The antibacterial effects of propolis results on S. aureus and Escherichia coli are in agreement with Shahbaz et al [1] and relatively higher than Afata et al [18]. Studies have linked propolis from Brazil, Egypt, Mangolia, and Albania to antibacterial activity against S. aureus, with zones of inhibition of 24, 21.8, 24.3, and 21.8 mm, respectively [1]. Different extraction techniques very certainly produce different chemical components, which could ultimately cause variations in the antibacterial activity [29].…”

“…The ethanolic extract of Malaysian propolis has been found to contain flavonoids, alkaloids, cardiac glycosides, tannins, saponins, phenol, xanthoproteins, terpenoids, and resins [19]. Variations in propolis composition are highly influenced due to phytogeographical diversity, climate change, seasonal variations, and specie of the queen bee [1]. Polyphenols are leading group of phytochemicals and deemed as active component of propolis.…”

“…Honeybees like Apis mellifera L. collect propolis, a sticky adhesive natural substance, from the buds, leaves, and other parts of plants and trees [ 1 ]. Propolis is rich in natural compounds which mainly depend on the type of plant accessible to the bees, geographical origin, and collection season.…”

Fatores De Heterogeneidade Do Potencial Antioxidante Da Própolis Da Abelha Apis Mellifera: Uma Revisão

“…Como a concentração de solventes usados para o preparo dos extratos de própolis infl uencia o rendimento da extração de compostos bioativos e, consequentemente, na sua atividade biológica (82), na Figura 10 estão os dados constantes nos trabalhos da presente revisão bibliográfi ca, para extratos etanólicos em água. Não estão presen-Figura 10.…”