Chemical profiling and total quality assessment of <i>Isodon japonica</i> using data-independent acquisition mode combined with superimposed multiple product ion UHPLC-Q-TOF-MS and chemometric analysis

Jin, Yiran; Tian, Tao; Ma, Yuhan; Hou, Ludan; Guan, Shuai; Sun, Wenjing; Du, Yingfeng; Zhang, Lantong

doi:10.1039/c8ra08732f

Cited by 5 publications

(7 citation statements)

References 23 publications

(11 reference statements)

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“…The contents of rosthornin A (11) and melissoidesin P (12) reached 2.90% and 1.55%, respectively. The contents of cirsiliol ( 13) cirsilineol ( 14) aurantiamide (15), and patriscabratine (16) were each less than 1%.…”

Section: Lc-ms/ms Analysis Of the Biologically Active Extractsmentioning

confidence: 95%

RETRACTED: In Vitro Antitumor Activities of Biologically Active Extracts and Isolated Phytochemicals From Rabdosia latifolia C. Y. Wu Et H. W. Li in Addenda

Liang

Zhang

et al. 2020

Natural Product Communications

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To investigate the antitumor activities, as well as phytochemical constituents of Rabdosia latifolia C. Y. Wu et H. W. Li in Addenda ( R. latifolia) extracts, the light petroleum, dichloromethane, ethyl acetate, n-butanol, and water extracts from R. latifolia were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays on MCF-7, HepG2, A549, Hela, and BGC-823 cells. We further examined cell cycle and apoptosis of the biologically active extracts on MCF-7 cells. The constituents of the biologically active extracts were determined using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Triple-TOF/MS) and gas chromatography–mass spectrometric (GC–MS) analysis. MTT assays showed that the dichloromethane extract had an inhibitory effect on MCF-7 and BGC-823 cells and that at 50 µg/mL showed 9.9% ± 1.7% inhibition of MCF-7 cells and 81.7% ± 9.5% of BGC-823 cells, respectively. The data for cell cycle analysis clearly showed a significant block in the S-phase. The dichloromethane extract induced apoptosis of MCF-7 cells, and the cell apoptosis rate showed time and concentration effects. UPLC- Triple-TOF/MS analysis revealed the presence of 16 structurally characterized compounds in the dichloromethane extract, all of which were identified for the first time in this species. Ent-kauranoids, such as henryin (9) and its analogs, were the predominant compounds. Subsequently, GC–MS analysis showed the presence of 20 volatile compounds, including γ-sitostenone, β-amyrone, γ-tocopherol, and dotriacontane. Based on the results of this study and previous literature reports, it is concluded that the ent-kauranoids, such as henryin, were the predominant components of R. latifolia with antitumor activity.

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Section: Lc-ms/ms Analysis Of the Biologically Active Extractsmentioning

confidence: 95%

RETRACTED: In Vitro Antitumor Activities of Biologically Active Extracts and Isolated Phytochemicals From Rabdosia latifolia C. Y. Wu Et H. W. Li in Addenda

Liang

Zhang

et al. 2020

Natural Product Communications

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“…According to the mass spectrometric data, compound 10 might be Oridonin based on the major ion at m/z 365, 347, 333. Compound 11 was likely to be Kamebanin, based primarily on its production of the ion at 315, 287, 269 [30] . Compound 12 was likely Rabdosin D, based on the major ion m/z 351 [31] .…”

Section: Resultsmentioning

confidence: 99%

“…By comparison of mass spectrometry data and standard data, compound 17 might be Glaucocalyxin A, based mainly on ions m/z 315, 223, and 124. Compound 18 was probably to be Sodoponin based on its predominant ion at 361, 347, 271 [30] …”

Section: Resultsmentioning

confidence: 99%

“…Compound 11 was likely to be Kamebanin, based primarily on its production of the ion at 315, 287, 269. [30] Compound 12 was likely Rabdosin D, based on the major ion m/z 351. [31] Based on the predominant ion at 375, 357, 314, compound 13 might be Leukamenin E. [29] Compound 14 could be Umbrosin A, based on the major ion m/z 315, 287, 271.…”

Section: Identification Of Potential Tyr Inhibitorsmentioning

confidence: 99%

“…[31] Based on the predominant ion at 375, 357, 314, compound 13 might be Leukamenin E. [29] Compound 14 could be Umbrosin A, based on the major ion m/z 315, 287, 271. [30] Compound 15 was likely to be Wangzaozin A, according to the ions generated at 333, 129 of it. [32] Compound 16 probably be Excisanin B, based on the major ion m/z 451, 331.…”

Section: Identification Of Potential Tyr Inhibitorsmentioning

confidence: 99%

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Screening and Capability Assessment of Tyrosinase Inhibitors in Isodon excisoides by Ultrafiltration Coupled with UHPLC‐Q‐TOF‐MS and Molecular Docking Technology

Jia

Sun

Zhang

et al. 2022

Chemistry & Biodiversity

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Tyrosinase inhibitors can alleviate the harm to the liver caused by tyrosinase. How to effectively screen out natural tyrosinase inhibitors becomes a focus. In this study, Isodon excisoides was first extracted with the ultrasound optimized by response surface methodology. Then, a method combined ultrafiltration with ultra‐liquid chromatography mass spectrometry (UHPLC/MS) was built to screen and identify tyrosinase inhibitors. The binding energies of active ingredients to tyrosinase were calculated by molecular docking. The reliability of the results was validated by the IC50 of enzyme inhibition assay. As a result, the binding energies of 7 components including excisanin B, lasiokaurin, rabdophyllin G, rabdoserrin B, rabdosin D, rabdosinate and weisiensin were lower than that of resveratrol. It was indicated that these components had high tyrosinase inhibitory activity. The IC50 values of lasiokaurin and excisanin B were 177 and 142 μmol/mL, which were less than that of resveratrol (183 μmol/mL). It showed that this way was simple, rapid, reliable and effective, which provided a new strategy to screen natural bioactive compounds from plants.

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Rapid discovery of potential ADR compounds from injection of total saponins from Panax notoginseng using data-independent acquisition untargeted metabolomics

et al. 2021

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Chemical profiling and total quality assessment of Isodon japonica using data-independent acquisition mode combined with superimposed multiple product ion UHPLC-Q-TOF-MS and chemometric analysis

Abstract: In this paper, a combined analytical strategy was developed and aimed at rapid characterization and quantitation of ent-kaurane diterpenoids in Isodon japonica using UPLC-Q-TOF-MS/MS.

Cited by 5 publications

References 23 publications

RETRACTED: In Vitro Antitumor Activities of Biologically Active Extracts and Isolated Phytochemicals From Rabdosia latifolia C. Y. Wu Et H. W. Li in Addenda

RETRACTED: In Vitro Antitumor Activities of Biologically Active Extracts and Isolated Phytochemicals From Rabdosia latifolia C. Y. Wu Et H. W. Li in Addenda

Screening and Capability Assessment of Tyrosinase Inhibitors in Isodon excisoides by Ultrafiltration Coupled with UHPLC‐Q‐TOF‐MS and Molecular Docking Technology

Rapid discovery of potential ADR compounds from injection of total saponins from Panax notoginseng using data-independent acquisition untargeted metabolomics

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