Rapid discovery of potential ADR compounds from injection of total saponins from Panax notoginseng using data-independent acquisition untargeted metabolomics

Wang, Chenxi; Pang, Xu; Zhu, Tongtong; Ma, Shuhua; Liang, Yunfei; Zhang, Yi; Xing, Lidan; Wang, Tao; Han, Lifeng

doi:10.1007/s00216-021-03734-5

Cited by 6 publications

(3 citation statements)

References 37 publications

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“…With the increased clinical application of injection of total saponins from P. notoginseng (ISPN), a modern preparation derived from TCM, adverse drug reactions and related safety issues have attracted much attention (Feng et al, 2021). A recent report was the first to demonstrate that Rg5, Rk1, and 20(S)‐ginsenoside Rh2 were potential compounds of clinical adverse drug reactions in the clinical practice of ISPN (Wang et al, 2022). To our best knowledge, a data‐independent acquisition strategy based on UHPLC–Q‐Orbitrap MS is beneficial to establish pharmacopeia standards and assess drug reactions in some homologous products of the Panax genus.…”

Section: Application Of Metabolomics In the Clinical Study Of Ginsengmentioning

confidence: 99%

Research progress on metabolomics in the quality evaluation and clinical study ofPanax ginseng

Xia

Sun

Wen

et al. 2022

Biomedical Chromatography

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Panax ginseng, an essential component of traditional medicine and often referred to as the king of herbs, has played a pivotal role in medicine globally for several millennia. Previously, traditional phytochemical methods were mainly used for quality evaluation and pharmacological mechanism studies of ginseng, resulting in the lack of systematicness and innovation and hindering the development and utilization of ginseng resources. Since the beginning of the new century, systems biology technology represented by metabolomics has shown unique advantages in the modernization and internationalization of herbal medicine, establishing a bridge for communication between traditional medicine and modern medicine. P. ginseng, a special herb used in medicine and food, is one of the main research objects for qualitative and quantitative analysis of metabolomics and has gradually become the focus of researchers globally. Here, we conducted a comprehensive summary and analysis of numerous studies published in ginseng metabolomics. This review aims to provide more novel ideas for the quality evaluation, development, and clinical application of ginseng in the future and offer more useful technical references for the modernization and internationalization of herbal medicine based on metabolomics.

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Section: Application Of Metabolomics In the Clinical Study Of Ginsengmentioning

confidence: 99%

Research progress on metabolomics in the quality evaluation and clinical study ofPanax ginseng

Xia

Sun

Wen

et al. 2022

Biomedical Chromatography

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“…G-Rh4 is a trace saponin in white ginseng, and it became the most abundant triol-type ginsenoside after the heating process. Compared with other saponins, G-Rh4 has relatively better water solubility ( Wang et al, 2022 ), suggesting its potential clinical applications. However, due to its low content in fresh or white ginseng and consequent preparation difficulty, there are few studies on the pharmacological effects of G-Rh4; especially, its anti-inflammatory activity and underlying mechanism are largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Integrative network pharmacology and experimental verification to reveal the anti-inflammatory mechanism of ginsenoside Rh4

Zhu

Wang

et al. 2022

Front. Pharmacol.

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Inflammation is an innate immune response to infection, and it is the main factor causing bodily injury and other complications in the pathological process. Ginsenoside Rh4 (G-Rh4), a minor ginsenoside of Panax ginseng C. A. Meyer and Panax notoginseng, has excellent pharmacological properties. However, many of its major pharmacological mechanisms, including anti-inflammatory actions, remain unrevealed. In this study, network pharmacology and an experimental approach were employed to elucidate the drug target and pathways of G-Rh4 in treating inflammation. The potential targets of G-Rh4 were selected from the multi-source databases, and 58 overlapping gene symbols related to G-Rh4 and inflammation were obtained for generating a protein–protein interaction (PPI) network. Molecular docking revealed the high affinities between key proteins and G-Rh4. Gene ontology (GO) and pathway enrichment analyses were used to analyze the screened core targets and explore the target–pathway networks. It was found that the JAK-STAT signaling pathway, TNF signaling pathway, NF-κB signaling pathway, and PI3K-Akt signaling pathway may be the key and main pathways of G-Rh4 to treat inflammation. Additionally, the potential molecular mechanisms of G-Rh4 predicted from network pharmacology analysis were validated in RAW264.7 cells. RT-PCR, Western blot, and ELISA analysis indicated that G-Rh4 significantly inhibited the production of pro-inflammatory cytokines such as TNF-α, IL-6, and IL-1β, as well as inflammation-related enzymes in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. Moreover, in vitro experiments evaluated that Ginsenoside Rh4 exerts anti-inflammatory effects via the NF-κB and STAT3 signaling pathways. It is believed that our study will provide the basic scientific evidence that G-Rh4 has potential anti-inflammatory effects for further clinical studies.

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“…In contrast to conventional injections, TCM injections have a similar incidence of serious ADRs (6.02% vs. 6.72%) but a much higher incidence of unknown ADRs (46.74% vs. 24.13%) (Li et al, 2019). Therefore, significant efforts have been devoted to developing platforms for toxicity evaluation of TCMs using genomic, metabolomic, microtoxicity techniques, or animal models, which have been extensively reviewed elsewhere (Gao et al, 2019;Xie et al, 2019;Yu et al, 2020;He et al, 2021;Tu et al, 2021;Jiang et al, 2022;Wang et al, 2022). However, unlike chemical or biological drugs, the complexity of TCM ingredients makes safety assessment extremely difficult.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial toxicity evaluation of traditional Chinese medicine injections with a dual in vitro approach

Shen

Guo

et al. 2022

Front. Pharmacol.

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Graphical AbstractA dual in vitro mitochondrial toxicity assay approach combing the conventional “glucose/galactose” assay in HepG2 cells with the cytotoxic assay in mitochondrial respiration deficient cells was established in this study. Using this platform, we systematically assessed the mitochondrial toxicity of TCM injections for the first time. Four TCM injections were identified with potential mitochondrial toxicity. Their toxic ingredients were predicted by molecular docking and validated by the dual in vitro approach.

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Rapid discovery of potential ADR compounds from injection of total saponins from Panax notoginseng using data-independent acquisition untargeted metabolomics

Cited by 6 publications

References 37 publications

Research progress on metabolomics in the quality evaluation and clinical study ofPanax ginseng

Research progress on metabolomics in the quality evaluation and clinical study ofPanax ginseng

Integrative network pharmacology and experimental verification to reveal the anti-inflammatory mechanism of ginsenoside Rh4

Mitochondrial toxicity evaluation of traditional Chinese medicine injections with a dual in vitro approach

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