Chemical Fingerprint and Quantitative Analysis for the Quality Evaluation of Docynia dcne Leaves by High-Performance Liquid Chromatography Coupled with Chemometrics Analysis

Abstract

Docynia dcne leaf from the genus of Docynia Dcne (including three species of Docynia delavayi, Docynia indica and Docynia longiunguis.) is an important raw material of local ethnic minority tea, ethnomedicines and food supplements in southwestern areas of China. However, D. dcne leaves from these three species are usually used confusingly, which could influence the therapeutic effect of it. A rapid and effective method for the chemical fingerprint and quantitative analysis to evaluate the quality of D. dcne leaves was established. The chemometric methods, including similarity analysis, hierarchical cluster analysis and partial least-squares discrimination analysis, were applied to distinguish 30 batches of D. dcne leaf samples from these three species. The above results could validate each other and successfully group these samples into three categories which were closely related to the species of D. dcne leaves. Moreover, isoquercitrin and phlorizin were screened as the chemical markers to evaluate the quality of D. dcne leaves from different species. And the contents of isoquercitrin and phlorizin varied remarkably in these samples, with ranges of 6.41-38.84 and 95.73-217.76 mg/g, respectively. All the results indicated that an integration method of chemical fingerprint couple with chemometrics analysis and quantitative assessment was a powerful and beneficial tool for quality control of D. dcne leaves, and could be applied also for differentiation and quality control of other herbal preparations.

Phlorizin ameliorates obesity-associated endotoxemia and insulin resistance in high-fat diet-fed mice by targeting the gut microbiota and intestinal barrier integrity

“…6 Our previous work found that Docynia indica could be an alternative source of PHZ, containing up to 25% of PHZ in dried leaves. 7 For over 160 years, PHZ has been used as a pharmaceutical tool in physiological research, as it is a competitive inhibitor of sodium-glucose co-transporter 2 (SGLT2), which is responsible for approximately 90% of glucose reabsorption. 6 In other words, PHZ holds great potential for improving hyperglycemia (one of the symptoms of metabolic disorders (MDs), like obesity and type 2 diabetes mellitus (T2DM)), by directly decreasing the blood glucose concentration rather than targeting insulin resistance and impaired insulin secretion.…”

The complete chloroplast genome sequence of Docynia indica (Wall.) Decne

“…Decne., also called Duo-Yi in China, is an evergreen wild fruit tree species belonging to the Rosaceae family, which wildly distributed from East Asia to China (Rymbai et al 2016). Its leaves are rich in polyphenols and flavonoids, extracts from the leaves have several pharmacological actions such as anti-obesity (Nguyen et al 2011), antifungal (Zhang et al 2018a), antioxidant (Sharma et al 2015), which were widely used as a medicine for the healing of empyrosis, fracture and fever by the local ethnic minorities in China (Lin et al 2003;Zhang et al 2018b). The fruits contain high levels of biologically active components including anti-oxidants, tannins and flavonoids, which were used as natural remedy to treat digestive problems and infectious diseases (Tiep et al 2018).…”

“…2011 ), antifungal (Zhang et al. 2018 a), antioxidant (Sharma et al. 2015 ), which were widely used as a medicine for the healing of empyrosis, fracture and fever by the local ethnic minorities in China (Lin et al.…”

Purified Phlorizin from DocynIa Indica (Wall.) Decne by HSCCC, Compared with Whole Extract, Phlorizin and Non-Phlorizin Fragment Ameliorate Obesity, Insulin Resistance, and Improves Intestinal Barrier Function in High-Fat-Diet-Fed Mice

“…Healthy young leaves of Docynia indica (Wall.) Decne were collected from the Pu’er (Yunnan, China) in 2014, which were identified and confirmed by Professor Qiang Luo from College of Xichang (Chengdu, Sichuan, China) according to the plant characteristics of DID [ 18 ]. The voucher specimen code is: PE-20141020.…”

“…It could be a widely used and cheap source of natural products with a high value. Our previous research has shown that the main polyphenols in DID is phlorizin (PHZ), with content much more than 40% in leaves [ 18 , 19 ], which was the first specific competitive inhibitor of sodium−glucose symporters (SGLTs) located in the mucosa of the small intestine (SGLT1) and the proximal renal tubule of the kidney (SGLT2), and was excellent for the treatment of type 2 diabetes [ 20 , 21 ]. Findings from Shin et al suggested that supplementation of PHZ could ameliorate obesity in high-fat diet (HFD)-induced obese mice [ 22 ].…”