Chemical composition of the leaf oil of Cleistopholis glauca Pierre ex Engler &amp; Diels from Côte d’Ivoire

et al. 2013

Phytochemical Analysis

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Section: Introductionmentioning

confidence: 99%

Analysis of Cleistopholis patens Leaf and Trunk Bark Oils Using Combined GC‐ Flame Ionisation Detection, GC‐Retention Index, GC–MS and ¹³C‐NMR

Ouattara

et al. 2013

Phytochemical Analysis

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“…Water distillation of root bark from C. glauca collected in Petit Yapo forest produced an essential oil with a yield of 0.04%, w/w, vs fresh material, much lower than that obtained from leaves (0.16-0.19%) [7]. The root bark oil was first analyzed by GC, in combination with retention indices (RI) on two capillary columns of different polarity and by 13 C NMR spectroscopy following a computerized method developed in our laboratory [8][9][10].…”

mentioning

confidence: 79%

“…Structural elucidation has been reported of compounds isolated from solvent extracts of C. glauca: oligorhamnosides [3,4]; lipids [5]; terpenes [4,5]; a phenyl propanoid [6]; and a furanic derivative [6]. The chemical composition of C. glauca leaf oil has been recently investigated by our group [7]. Oil samples isolated from leaves collected from individual trees were analyzed by gas chromatography (GC) using a flame ionisation detector (FID), and the compounds were characterized by a combination of retention indices (RI) and 13 C NMR spectroscopy.…”

mentioning

confidence: 99%

Combined Analysis of the Root Bark Oil of Cleistopholis glauca by Chromatographic and Spectroscopic Techniques

Ouattar

Natural Product Communications

et al. 2013

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The composition of root bark oil from Cleistopholis glauca Pierre ex Engler & Diels growing wild in Ivory Coast was investigated by GC (in combination with retention indices) and 13 C NMR spectroscopy after partition of hydrocarbons and oxygenated compounds on silica gel. Thirty-one compounds have been identified. C. glauca produces a sesquiterpene-rich oil, patchoulenone (33.5%), cyperene (9.5%) and germacrene D (6.6%) being the main components. Special attention was paid to the identification and quantification of germacrene C (a heat-sensitive compound) and -elemene, which were achieved by a combination of GC(FID) and 13 C NMR spectroscopy. The composition of C. glauca root bark and leaf oils differed drastically.

“…In continuation of our on‐going work on the characterization of aromatic plants of the Ivory Coast through the chemical composition of their essential oils, we conducted this study on the leaf oil from P. longifolia . In this paper we highlight the key role of 13 C nuclear magnetic resonance (NMR) (in combination with column chromatography) in the identification of oxygenated sesquiterpenes whose mass data are not compiled in commercial computerized mass spectrometry (MS) libraries.…”

Section: Introductionmentioning

confidence: 99%

The key role of ¹³C NMR analysis in the identification of individual components of Polyalthia longifolia leaf oil

Ouattara

et al. 2014

Flavour & Fragrance J

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Polyalthia longifolia produces sesquiterpene-rich essential oils (EOs) whose compositions varied substantially from sample to sample depending on the origin of the plant (Nigeria and Vietnam). Nothing is known about the phytochemistry of Ivoirian P. longifolia. The aim of the present study was to characterize Ivoirian P. longifolia through the chemical composition of the leaf oil and to develop a strategy that allows the identification of minor oxygenated sesquiterpenes whose MS data are not compiled in commercial or laboratory-constructed MS libraries. The EO was submitted to gas chromatography (GC) retention index (RI), GC-mass spectrometry (MS) and 13 C nuclear magnetic resonance (NMR) analysis. Then hydrocarbons and oxygenated components were separated and the oxygenated fraction was chromatographed on silica gel. The fractions were analysed by GC(RI) and 13 C NMR. Seventy compounds accounting for 91.8% of the EO were identified. Sesquiterpene hydrocarbons, (E)-β-caryophyllene (27.8%), α-zingiberene (20.0%) and allo-aromadendrene (15.0%), were the major components. Various oxygenated sesquiterpenes whose MS data were not compiled in commercial and laboratory-made MS libraries were identified by comparison of their chemical shift values in the spectrum of the fraction of CC with those reported in the literature and compiled in a laboratory-constructed 13 C NMR data library. The composition of the investigated Ivoirian P. longifolia oil sample presented similarities and differences with Nigerian and Vietnamese oils. Combined analysis of Ivoirian P. longifolia EO by chromatographic and spectroscopic techniques including 13 C NMR without isolation of the components, appeared particularly efficient to identify minor components of EOs, whose MS spectra are insufficiently differentiated or MS data are not compiled in commercial and lab-constructed MS libraries.