Combined Analysis of the Root Bark Oil of Cleistopholis glauca by Chromatographic and Spectroscopic Techniques

Abstract

The composition of root bark oil from Cleistopholis glauca Pierre ex Engler & Diels growing wild in Ivory Coast was investigated by GC (in combination with retention indices) and 13 C NMR spectroscopy after partition of hydrocarbons and oxygenated compounds on silica gel. Thirty-one compounds have been identified. C. glauca produces a sesquiterpene-rich oil, patchoulenone (33.5%), cyperene (9.5%) and germacrene D (6.6%) being the main components. Special attention was paid to the identification and quantification of germacrene C (a heat-sensitive compound) and -elemene, which were achieved by a combination of GC(FID) and 13 C NMR spectroscopy. The composition of C. glauca root bark and leaf oils differed drastically.

Composition and Chemical Variability of Root Bark oil from Ivoirian Cleistopholis patens

“…In contrast, the composition dominated by patchoulenone is reported here for the first time in C. patens essential oils. This ketone has been previously identified at a lesser extent (33.2%) in C. glauca root bark oil [20].…”

“…From the analytical point of view, combined analysis of essential oils by GC(RI), GC-MS and 13 C NMR appeared useful. Analyses: GC, GC-MS and 13 C NMR analyses were performed as previously reported [20,21].…”