Chemical composition, cytotoxicity, and antibacterial activity of propolis from Africanized honeybees and three different Meliponini species

Surek, Mônica; Fachi, Mariana Millan; Cobre, Alexandre de Fátima; Oliveira, Favízia Freitas de; Pontarolo, Roberto; Crisma, Amanda Rabello; Souza, Wladmir F.

doi:10.1016/j.jep.2020.113662

Cited by 39 publications

(24 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Propolis was effective against tumor cell lines. They concluded that propolis is a substance with antineoplastic properties [ 33 ]. Mohamed et al (2020) studied the cytotoxic properties of some propolis samples that were investigated by MTT assay on MCF7 and MCF 10A.…”

Section: Discussionmentioning

confidence: 99%

Chemical Characterization and Cytotoxic/Antibacterial Effects of Nine Iranian Propolis Extracts on Human Fibroblast Cells and Oral Bacteria

Yazdanian

Motallaei

Tahmasebi

et al. 2022

BioMed Research International

View full text Add to dashboard Cite

Multimicrobial infections caused by pathobionts are called dysbiotic multimicrobial illnesses. Commercial mouthwashes, such as chlorhexidine, have negative side effects that can prevent tooth decay and infection. The present study aimed to determine the antifungal, antibacterial, and cytotoxicity characteristics of the propolis extracts from different areas (Iran). The ethanolic extract of propolis was prepared. GC/MS carried out the characterization to determine the thymol, carvacrol, and menthol extracts, and also, total phenol and flavonoid were assed for all samples. The antimicrobial and antibiofilm effects were evaluated against S. mutans, S. mitis, S. salivarius, L. acidophilus, E. coli, S. aureus, and C. albicans. The cytotoxic effect of extracts was measured on human fibroblast cells by MTT test. The MIC values in mg mL-1 were ranged as follows: S. salivarius (0.003 to 0.048), S. mutans (0.003 to 0.029), S. mitis (0.007 to 0.058), L. acidophilus (0.007 to 0.117), C. albicans (0.014 to 0.234), E. coli (0.007 to 0.058), and S. aureus (0.007 to 0.058), while MBC were, respectively, S. mutans (0.007 to 0.058), S. salivarius (0.007 to 0.117), S. mitis (0.007 to 0.117), L. acidophilus (0.014 to 0.234), C. albicans (0.029 to 0.468), E. coli (0.014 to 0.234), and S. aureus (0.007 to 0.117). Cariogenic bacteria and Candida albicans were demonstrated to be resistant to propolis extracts. Therefore, propolis extracts may make good mouthwashes.

show abstract

Section: Discussionmentioning

confidence: 99%

Chemical Characterization and Cytotoxic/Antibacterial Effects of Nine Iranian Propolis Extracts on Human Fibroblast Cells and Oral Bacteria

Yazdanian

Motallaei

Tahmasebi

et al. 2022

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…Enzymes such as succinic dehydrogenase, glucose-6-phosphate, adenosine triphosphate and acid phosphate have been detected in a variety of propolis types. The chemical compositions of varieties of propolis from different geographical and botanical niches show striking variability, posing additional challenges in the application of propolis in health/cosmetic/pharmaceutical industries [ 10 , 11 , 12 , 13 , 14 ].…”

Section: Propolis Constituentsmentioning

confidence: 99%

Experimental Evidence for Therapeutic Potentials of Propolis

et al. 2021

View full text Add to dashboard Cite

Propolis is produced by honeybees from materials collected from plants they visit. It is a resinous material having mixtures of wax and bee enzymes. Propolis is also known as bee glue and used by bees as a building material in their hives, for blocking holes and cracks, repairing the combs and strengthening their thin borders. It has been extensively used since ancient times for different purposes in traditional human healthcare practices. The quality and composition of propolis depend on its geographic location, climatic zone and local flora. The New Zealand and Brazilian green propolis are the two main kinds that have been extensively studied in recent years. Their bioactive components have been found to possess a variety of therapeutic potentials. It was found that Brazilian green propolis improves the cognitive functions of mild cognitive impairments in patients living at high altitude and protects them from neurodegenerative damage through its antioxidant properties. It possesses artepillin C (ARC) as the key component, also known to possess anticancer potential. The New Zealand propolis contains caffeic acid phenethyl ester (CAPE) as the main bioactive with multiple therapeutic potentials. Our lab performed in vitro and in vivo assays on the extracts prepared from New Zealand and Brazilian propolis and their active ingredients. We provided experimental evidence that these extracts possess anticancer, antistress and hypoxia-modulating activities. Furthermore, their conjugation with γCD proved to be more effective. In the present review, we portray the experimental evidence showing that propolis has the potential to be a candidate drug for different ailments and improve the quality of life.

show abstract

“…Antimicrobial synergism is also a hot topic for scientists and clinicians as combinations of antimicrobials can be more effective in the treatment of polymicrobial infections, in preventing the prevalence of resistant microorganisms and in reducing a possible dose‐related toxicity to humans (Mehta et al, 2014). Several authors have reported propolis synergisms with different types of antibiotics like the ones belonging to the aminoglycoside class that are inhibitors of protein synthesis (Table 1; AL‐safi, 2013; Fernandes Júnior et al, 2005; Grecka et al, 2019; de Lima Silva et al, 2015; Neto et al, 2017; Okińczyc et al, 2020; Runyoro et al, 2017; Scazzocchio et al, 2006; Scheller et al, 1999; Stepanović et al, 2003; Surek et al, 2021; Wojtyczka et al, 2013), showing propolis potential to be used as an adjuvant in antibiotic therapy. However, to the best of our knowledge, such synergisms were never reported for Portuguese propolis.…”

Section: Introductionmentioning

confidence: 99%

Propolis antibacterial and antioxidant synergisms with gentamicin and honey

Freitas

Cunha

Oliveira

et al. 2022

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aims: Assess the antibacterial and antioxidant effects of ethanol extracts of Portuguese propolis samples when combined with gentamicin, a widely used aminoglycoside antibiotic, or with honey, collected from the same apiary as propolis. Methods and Results:Using the agar dilution method and DPPH free radical scavenging assay, antimicrobial and antioxidant synergistic effects between propolis and gentamicin or honey were assessed. Synergism between propolis and gentamicin was observed for all the tested extracts and against all the indicator bacteria, with particular interest against the Methicillin-Resistant Staphylococcus aureus (MRSA) with a threefold decrease of the gentamicin MIC if mixed with 25 µg ml −1 propolis.Likely to propolis and gentamicin, mixtures of sub-MIC concentrations of propolis and honey enhanced the antibacterial action of each individual natural product against the majority of the strains. However, propolis antioxidant capacity decreased along with higher honey content in the mixture.Conclusions: Propolis has a strong synergistic effect when combined with gentamicin, allowing the reduction of the therapeutic dose of this drug. Propolis and honey mixtures also display a stronger antibacterial effect than the activity exhibited by each sample when tested individually, whereas the high antioxidant capacity of propolis seems to be not affected when in combination with honey. Significance and Impact of Study: When mixed with honey, propolis antioxidant potential is maintained, or just slightly reduced up to 1:1 dilution, and show synergistic antibacterial effects, allowing to optimize the use of this usually scarce natural resource. Also, considering the antibiotic resistance problem, natural beehive products, alone or in the mixture, are promising alternatives to retard the outbreak of microbial resistance.

show abstract

Chemical composition, cytotoxicity, and antibacterial activity of propolis from Africanized honeybees and three different Meliponini species

Cited by 39 publications

References 56 publications

Chemical Characterization and Cytotoxic/Antibacterial Effects of Nine Iranian Propolis Extracts on Human Fibroblast Cells and Oral Bacteria

Chemical Characterization and Cytotoxic/Antibacterial Effects of Nine Iranian Propolis Extracts on Human Fibroblast Cells and Oral Bacteria

Experimental Evidence for Therapeutic Potentials of Propolis

Propolis antibacterial and antioxidant synergisms with gentamicin and honey

Contact Info

Product

Resources

About