Chemical composition, antibacterial and antifungal activities ofTrichoderma sp. growing in Tunisia

Khethr, Fethi Bel Haj; Ammar, Samia; Saïdana, Dhouha; Daami, Majda; Chriaa, Jihane; Liouane, Kaouthar; Mahjoub, Mohamed Ali; Helal, Ahmed Noureddine; Mighri, Zine

doi:10.1007/bf03175334

Cited by 18 publications

(9 citation statements)

References 32 publications

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“…Earlier, in the studies of Trichoderma activity, a number of authors concluded that gram-negative bacteria were more sensitive to the antibacterial action of Trichoderma species than Gram-positive ones [16,17]. As for our data, this conclusion is not confi rmed because most of the studied fungal strains showed antibacterial activity against C. michiganensis subsp.…”

Section: Methods Of Diff Usion In Agarcontrasting

confidence: 75%

Antibacterial Activity of Different Strains of the Genus Trichoderma

Kurchenko¹,

Yurieva²,

Syrchyn³

et al. 2023

Mikrobiol. Z.

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The main pathogens causing plant diseases are bacteria, viruses, and fungi. A number of strategies are usually used for plant protection and control of pathogenic microorganisms. The main interest of researchers is focused on the development of alternative synthetic chemicals to control bacterial diseases of plants. Among such approaches, biological control of bacterial diseases using agents such as antagonistic fungi and some other microorganisms is considered to be one of the most effective strategies. Species of the genus Trichoderma are known for their antagonistic activity against plant pathogenic fungi and bacteria and can be an effective safety strategy to control them. An important peculiarity of fungi of this genus is their ability to inhibit target pathogenic organisms without harming non-target (beneficial) microorganisms. The study of the antagonistic activity of fungi of the genus Trichoderma was conducted mainly against pathogenic fungi of agricultural plants. At the same time, the study of the antibacterial activity of fungi of this genus has attracted much less attention. Therefore, the aim of our work was to determine the antibacterial activity of microscopic fungi of the genus Trichoderma against test cultures of bacteria causing pathogenesis of agricultural plants. Methods. The objects of research were 100 fungal strains of the genus Trichoderma and six economically important plant pathogenic bacteria such as Pseudomonas syringae UCM В-1027Т, Pseudomonas fluorescens 8573, Pectobacterium carotovorum UCM В-1095T, Xanthomonas campestris pv. campestris UCM В-1049, Clavibacter michiganensis subsp. michiganensis 102, and Agrobacterium tumefaciens UCM В-1000. Cultures of the studied fungi were grown on potato-dextrose agar. The antagonistic activity of fungi of the genus Trichoderma against plant pathogenic bacteria was studied using the conventional method of diffusion in agar and method of dual culture. The antibacterial activity of culture filtrates of Trichoderma strains was evaluated via the zone of growth inhibition of plant pathogenic bacteria. The percentages of growth inhibition of plant pathogenic bacteria were calculated, and the antagonistic activity of strains was concluded on the basis of the obtained values. Results. In general, the studied Trichoderma strains had the antagonistic activity against plant pathogenic bacteria. Using method of diffusion in agar, it was shown that among the 100 studied Trichoderma strains, 12 had the effect of growth inhibition (bacteriostatic effect) of all six studied species of pathogenic bacteria; 20 strains inhibited the growth of five ones, 36 — four, 12 — three, and 7 — of two strains. The strains with a wide spectrum of antibacterial activity were studied by the double culture method. This made it possible to demonstrate the high selectivity of the antagonistic effect of Trichoderma strains on individual test cultures of phytopathogenic bacteria. For example, strain No7A inhibited the growth of C. michiganensis subsp. michiganensis 102 by 47% and the growth of P. syringae UCM B-1027T by 30%, while the zones of growth inhibition of these test cultures, determined by the method of diffusion in agar, were 5 and 6 mm, respectively. Conclusions. The obtained results indicated the potential and overall ability of Trichoderma strains to biologically control bacterial pathogens. The most promising for the use of plant pathogenic bacteria as agents for biocontrol were strains F-60, 1515, and 320, which were active against all studied bacteria. Such strains may have the potential as a preventive biocontrol agent of plant pathogens with a wide range of action. On the other hand, Trichoderma strains with high activity against certain pathogens may have the potential to be used as a control agent against a specific target pathogen.

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Section: Methods Of Diff Usion In Agarcontrasting

confidence: 75%

Antibacterial Activity of Different Strains of the Genus Trichoderma

Kurchenko¹,

Yurieva²,

Syrchyn³

et al. 2023

Mikrobiol. Z.

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“…The inocula of the respective bacteria were streaked into MH agar plates using a sterile swab and were then dried at 37°C during 15 min. A sterile filter disc having 6 mm of diameter was placed at the surface of MH agar and 5 μl of the essential oil was dropped onto each Whatman paper disc [30]. The treated Petri dishes were incubated at 37°C for 18 to 24 h. The antibacterial activity was evaluated by measuring the clear zone surrounding the Whatman paper.…”

Section: Methodsmentioning

confidence: 99%

The in-vitro evaluation of antibacterial, antifungal and cytotoxic properties of Marrubium vulgare L. essential oil grown in Tunisia

et al. 2011

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BackgroundIn order to validate its antiseptic and anticancer properties with respect to traditional uses, we have screened for the first time the antimicrobial activity of aerial parts of M. vulgare L. essential oil against different pathogenic microorganisms and the cytotoxic activity against HeLa cell lines.MethodsThe agar disk diffusion method was used to study the antibacterial activity of M. vulgare essential oil against 12 bacterial and 4 fungi strains. The disc diameters of zone of inhibition (DD), the minimum inhibitory concentrations (MIC) and the concentration inhibiting 50% (IC50) were investigated to characterize the antimicrobial activities of this essential oil. The in vitro cytotoxicity of M. vulgare essential oil was examined using a modified MTT assay; the viability and the IC50 were used to evaluate this test.ResultsThe antimicrobial activity of the essential oil was investigated in order to evaluate its efficacy against the different tested microorganisms. The present results results showed a significant activity against microorganisms especially Gram (+) bacteria with inhibition zones and minimal inhibitory concentration values in the range of 6.6-25.2 mm and 1120-2600 μg/ml, respectively, whereas Gram (-) bacteria exhibited a higher resistance. As far as the antifungal activity, among four strains tested, Botrytis cinerea exhibited the strongest activity with inhibition zones of 12.6 mm. However, Fusarium solani, Penicillium digitatum and Aspergillus niger were less sensitive to M. vulgare essential oil. About the citotoxicity assay, this finding indicate the capability of this essential oil to inhibited the proliferation of HeLa cell lines under some conditions with IC50 value of 0.258 μg/ml.ConclusionThis investigation showed that the M. vulgare essential oil has a potent antimicrobial activity against some Gram (+) pathogenic bacteria and Botrytis cinerea fungi. The present studies confirm the use of this essential oil as anticancer agent. Further research is required to evaluate the practical values of therapeutic applications.

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“…The agar diffusion method was used for the determination of antibacterial activities of P. canariensis seeds oils according to the method described by Bel Haj Khether et al 18 . The bacterial cultures were first grown on Muller Hinton agar MH plates at 37 for 18 to 24 h prior to seedsing onto the nutrient agar.…”

Section: Determination Of Antibacterial Activitymentioning

confidence: 99%

Chemical Composition, Characteristics Profiles and Bioactivities of Tunisian <i>Phalaris canariensis</i> Seeds: a Potential Source of ω-6 and ω-9 Fatty Acids

Salah

Kchaou

Kolsi³

et al. 2018

J. Oleo Sci.

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Seeds oils of Phalaris canariensis extracted by ultrasonication and cold maceration were evaluated for their physical characteristics, total phenol contents, fatty acid and sterol compositions as well as for their antioxidant, antibacterial and acetylcholinesterase activities. The physicochemical properties of ultrasonication and cold maceration oils respectively were: acid values (4.00 and 3.25) mg KOH/g, peroxide values (5.53 and 4.41) meq O2 Kg, iodine values (88.83 and 95.17) g/100 g of oil, saponification values (119.21 and 98.17) mg KOH/g, phenolic content (36.40 and 53.00) mg GAE/g extract, chlorophylls (0.52 and 0.60) mg/kg oil and carotenoids contents (1.92 and 1.88) mg/kg oil. Gas chromatography analysis revealed that linoleic (52.03 and 52.2%), oleic (31.75 and 31.84%) and palmitic (11.09 and 11.34 %) acids were the major fatty acids in the two oils. Specific extinctions at 232 nm (K) and 270 nm (K) were (0.58 and 0.44) and (0.42 and 0.33), respectively. The DSC melting curve showed that their melting points and melting enthalpies were (-28.05°C and 76.8 J/g) and (-27.47°C and 62.3 J/g), respectively. On the other hand, the evaluation of their DPPH radical scavenging, total antioxidant capacity, antibacterial and acetylcholinesterase activities showed interesting results. Thus, Phalaris canariensis seeds oils could deserve further consideration and investigation as a potentially new multi-purpose product for agro-food, medicinal and cosmetic uses.

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Chemical composition, antibacterial and antifungal activities ofTrichoderma sp. growing in Tunisia

Cited by 18 publications

References 32 publications

Antibacterial Activity of Different Strains of the Genus Trichoderma

Antibacterial Activity of Different Strains of the Genus Trichoderma

The in-vitro evaluation of antibacterial, antifungal and cytotoxic properties of Marrubium vulgare L. essential oil grown in Tunisia

Chemical Composition, Characteristics Profiles and Bioactivities of Tunisian <i>Phalaris canariensis</i> Seeds: a Potential Source of ω-6 and ω-9 Fatty Acids

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