Chemical Composition and Cytotoxic Activity of the Essential Oil and Oleoresins of In Vitro Micropropagated Ansellia africana Lindl: A Vulnerable Medicinal Orchid of Africa

Abstract

Orchids are rich treasure troves of various important phytomolecules. Among the various medicinal orchids, Ansellia africana stands out prominently in the preparing of various herbal medicines due to its high therapeutic importance. The nodal explants of A. africana were sampled from asymbiotically germinated seedlings on basal Murashige and Skoog (MS) medium and were micropropagated in MS medium supplemented with 3% sucrose and 10 µM meta topolin (mT) + 5 µM naphthalene acetic acid (NAA) +15 µM indole butyric acid (IBA) + 30 µM phloroglucinol (PG). In the present study, the essential oil was extracted by hydrodistillation and the oleoresins by the solvent extraction method from the micropropagated A. africana. The essential oil and the oleoresins were analysed by Gas Chromatography (GC) and GC/MS (Mass spectrometry). A total of 84 compounds were identified. The most predominant components among them were linoleic acid (18.42%), l-ascorbyl 2,6-dipalmitate (11.50%), linolenic acid (10.98%) and p-cresol (9.99%) in the essential oil; and eicosane (26.34%), n-butyl acetate (21.13%), heptadecane (16.48%) and 2-pentanone, 4-hydroxy-4-methyl (11.13%) were detected in the acetone extract; heptadecane (9.40%), heneicosane (9.45%), eicosane (6.40%), n-butyl acetate (14.34%) and styrene (22.20%) were identified and quantified in the ethyl acetate extract. The cytotoxic activity of essential oil and oleoresins of micropropagated A. africana was evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide) assay on Vero cells compared to the standard drug doxorubicin chloride. The present research contains primary information about the therapeutic utility of the essential oil and oleoresins of A. africana with a promising future research potential of qualitative and quantitative improvement through synchronised use of biotechnological techniques.

Phytochemical Analysis of Anacamptis coriophora Plant Cultivated Using Ex Vitro Symbiotic Propagation

“…[47] In a previous study, linoleic acid was detected as 18.42 % in the medicinal orchid Ansellia africana species. [48] This rate was found to be similar in the flower part of A. coriphora. Another major component, oleic acid, is an important fatty acid obtained from plant sources.…”

“…Alpha‐linolenic acid is essential for the healthy growth and function of cells [47] . In a previous study, linoleic acid was detected as 18.42 % in the medicinal orchid Ansellia africana species [48] . This rate was found to be similar in the flower part of A. coriphora .…”

Chemical Compositions and Experimental and Computational Modeling of the Anticancer Effects of Cnidocyte Venoms of Jellyfish Cassiopea andromeda and Catostylus mosaicus on Human Adenocarcinoma A549 Cells

“…The following supporting information can be downloaded at: : Figure S1: GC-MS chromatogram of the venom of Cassiopea andromeda and Catostylus mosaicus ; Table S1: GC-MS compounds in the venom of Cassiopea andromeda ; Table S2: GC-MS compounds in the venom of Catostylus mosaicus ; Table S3: Receptors involve in the apoptosis of cells of human pulmonary adenocarcinoma cells (A59); Western blot original gels [ 32 , 33 , 36 , 43 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 ]. Video S1: Cnidosomes of Cassiopea andromeda and Catostylus mosaicus and their motility through the mucus secreted by the jellyfish.…”

Phytochemical and cytotoxic study in Quisqualis indica volatile oil cultivation in Iraq

“…

The volatile oil is metabolites product from some plant have biological activities such as antimicrobial ,antioxidant and have viability for reduced cancer cell ,through inhibit cell proliferation ,for this reason This study was set to extraction volatile oils from Inflorescences of Qusiqualis indica plant cultivation in Iraq from tow season (spring ,autumn)and identification of the Chemical compounds of these ( using GC-MS ) ,The cytotoxic effect of two seasons volatile oils on human lung cancer cells A549 compared with normal REF cell cells were studied at concentrations (5,6,12,25,50)μl/ml and the programmed cell death (apoptosis) tested for cells lines and measured P53 gene ,Caspase-8 expression levels. The results showed significant quantitative and quantitative in two seasons oils ,and there were significant differences in the degree of cytotoxicity between the two oils ,where is at the concentration (50 μl/ml)they have the highest effects of(A549)cells compared with REF cells ,while the higher apoptosis effect at (25 μl/ml) concentrate for A549 compared with REF.

…”