Chemical Composition and Biological Activities of The Essential Oil And Anatomical Markers Of Lavandula Dentata L. Cultivated In Brazil

Abstract

Lavandula dentata, popularly known as lavender, is commonly used in traditional medicine for the treatment of digestive and inflammatory disorders. The objective of this study was to analyzed the chemical oil composition, antioxidant and antimicrobial activities of the essential oil and anatomical markers of the leaf and stem of L. dentata cultivated in South Brazil. Essential oil showed an antioxidant activity similar to rutin and gallic acid when analyzed by phosphomolybdenum method. However, by the free radical DPPH and ABTS methods, it showed a slight potential antioxidant. Essential oil presented 1,8-cineol (63%) as major component, antimicrobial activity against Gram-positive, Gram-negative bacteria strains and Candida albicans, by broth microdilution. The anatomical profile provided the following main microscopic markers: hypostomatic leaves; diacytic stomata, thin and striate cuticle; multicellular and branched non-glandular trichomes; capitate glandular trichomes; peltate glandular trichomes; dorsiventral mesophyll; flat-convex shape midrib, truncated on the abaxial side; one collateral vascular bundle in the midrib; square stem shape, angular collenchyma alternated with cortical parenchyma; sclerenchymatic fibers well-developed on the four edges.

Anatomy, micromorphology, and histochemistry of leaves and stems of Cantinoa althaeifolia (Lamiaceae)

“…This content was detected using Sudan III in the histochemical test. This kind of trichome is commonly found in Lamiaceae species (Chatri et al, ; Gul et al, ; Justus et al, ; Kowalczuk et al, ; Talebi et al, ).…”

“…Diacytic stomata are present only on abaxial side (Figure c). Amphistomatic leaves are frequent in Lamiaceae (Metcalfe & Chalk, ), yet hypostomatic leaves can also be found, such as in Stachys yemenensis Hedge (Ya'ni, Hassan, Elwan, Ibrahim, & Eldahshan, ) and Lavandula dentata L. (Justus et al, ). This characteristic is helpful in the diagnosis of C. althaeifolia .…”

“…In the spongy parenchyma, collateral vascular bundles are present and are delimited by a parenchymatous sheath (Figure c,e). Dorsiventral mesophyll has been observed in numerous Lamiaceae species (Justus et al, ; Ya'ni et al, ).…”

“…(Seyedi & Salmaki, ), Hyptis villosa Pohl ex Benth. (Tozin & Rodrigues, ), Salvia divinorum Epling & Játiva (Kowalczuk et al, ), S. nemorosa L. (Talebi et al, ) and L. dentata (Justus et al, ).…”

“…Different shapes of stems have been described for species of Lamiaceae, for example, square shape in S. schimperi , semicircular in Thymus serpyllum L., square with two concave and two convex lateral sides in Origanum majorana L., and square with four protruding angles in Lavandula pubescens Decne. (Ya'ni et al, ) and L. dentata (Justus et al, ).…”