Chemical composition and biological activities of Indigofera hirsuta aerial parts’ methanol fractions

Abstract

Methanol extract of Indigofera hirsuta, was evaluated for its antiradical potential and capacity in inhibiting lipoxygenase and aldose/aldehyde reductase enzymes. The ethyl acetate fraction derived from the methanol extract partition, showed the greatest antioxidant capacity, while the butanol was the strongest inhibitor of lipoxygenase enzyme. All fractions (diethyl ether, ethyl acetate, butanol and the aqueous residue) exhibited strong inhibition capacity of both aldose/aldehyde reductase enzymes, which comes in agreement with the ethnomedicinal plant utilization as an antidiabetic agent.LC-DAD-MS(ESI+) fraction analysis verified the findings above, leading to a conclusion regarding the biological activities attributed to the main compounds.Phytochemical analysis led to the identification of an indolic dimer, cinnamic acids, phenolics, flavonoid glycosides, a cyclic polyol, the rare sugar 1-methyl-β-Dglucopyranoside and glycerol. Many of these compounds were isolated for the first time in Indigofera species while the indolic dimer was isolated for the first time in the Fabaceae family.

A Review on Exploring the Phytochemical and Pharmacological Significance of Indigofera astragalina

“…Through phytochemical analysis, researchers have identified several bioactive compounds present in the methanol extract of I. hirsuta . These constituents include a dimer of indole derivatives, flavonoid glycosides, a polyol cyclical product, 1-methyl-β- d -glucopyranoside, and cinnamic acids (Karakousi et al, 2020). It is noteworthy that most of these phytoconstituents were elucidated from Indigofera sp., while the dimer of the indole derivative represents a novel discovery within the Fabaceae family, especially I. astragalina.…”