Chemical characterization of the essential oil from patchouli accessions harvested over four seasons

ABSTRACT. Due to the economic interests in vetiver, Chrysopogon zizanioides (L.) Roberty, molecular and chemical studies are essential to generate information for its sustainable exploitation. The aim of this study was to undertake a molecular and chemical characterization of vetiver accessions of the active germplasm bank of the Universidade Federal de Sergipe. The molecular characteristics of the accessions were studied using amplified fragment length polymorphism markers, with a total of 14 primer combinations that generated 442 loci, allowing us to observe that these accessions have similar genomes. The vetiver accessions were divided into three distinct groups, where accession UFS-VET005 was the most differentiated and accession UFS-VET004 had the lowest essential oil content (0.70%). The content of the chemical constituents of the essential oils was observed to vary, with a predominance of khusimol, which ranged from 18.97 to 25.02%. It was possible to divide the vetiver accessions into two groups based on chemical composition, and these groups do not correlate with the molecular grouping. Therefore, it is necessary to perform molecular and chemical analyses to characterize vetiver accessions.

“…A grouping analysis with a similar method was performed with Pogostemon sp. (Blank et al, 2011), which showed good consistency in the established chemical groups.…”

Section: Chemical Characterization Of the Essential Oilmentioning

confidence: 52%

Section: Chemical Characterization Of the Essential Oilmentioning

confidence: 99%

Molecular and chemical characterization of vetiver, Chrysopogon zizanioides (L.) Roberty, germplasm

Celestino¹,

Zucchi²,

Pinheiro³

et al. 2015

“…The 10 accessions were divided into two clusters based on the results obtained by Blank et al (2011): one cluster consisted of POG-001 and POG-006 and the other cluster consisted of the remaining 8 accessions. In the statistical-genetic analyses, the allele frequency, observed heterozygosity (H O ), expected heterozygosity (H E ), allele amplitude, and polymorphic information content (PIC) were determined using MStools (Park, 2001).…”

Section: Molecular Analyses With Ssr Markersmentioning

confidence: 99%

“…When evaluating chemical variations of the essential oil of the patchouli accessions in the Active Germplasm Bank of Universidade Federal de Sergipe (except POG-017), Blank et al (2011) detected two clusters through multivariate analyses of four harvests, with POG-001 and POG-006 forming the first cluster (P. heyneanus), and the remaining accessions forming the second cluster (P. cablin).…”

Section: Molecular Analyses With Issr Markersmentioning

confidence: 99%

Molecular characterization of patchouli (Pogostemon spp) germplasm

Sandes¹,

Zucchi²,

Pinheiro³

et al. 2016

ABSTRACT. Patchouli [Pogostemon cablin (Blanco) Benth.] is an aromatic, herbaceous plant belonging to the Lamiaceae family native to Southeast Asia. Its leaves produce an essential oil regularly used by the perfume and cosmetics industries. However, since patchouli from the Philippines and India were described and named Pogostemon patchouli, there has been a divergence in the identity of these species. The objective of the current study was to study the genetic diversity of patchouli accessions in the Active Germplasm Bank of Universidade Federal de Sergipe using microsatellite and inter simple sequence repeat markers. The results of both types of molecular markers showed that there are two well-defined clusters of accessions that harbor exclusive alleles. It was observed that these two clusters are genetically distant, suggesting that they belong to

“…A patchouli collection from Universidade Federal de Sergipe (UFS) has been chemically and morpho-agronomically characterized (Blank et al, 2011). However, for complete characterization of a germplasm bank, molecular markers are an efficient alternative.…”

Section: Introductionmentioning

confidence: 99%

Short Communication Development and characterization of microsatellite primers in Pogostemon cablin (Lamiaceae)

Ss¹,

Jb²,

Zucchi³

et al. 2013

ABSTRACT. Microsatellite primers were developed and optimized for patchouli (Pogostemon cablin) to characterize the patchouli Active Germplasm Bank of Universidade Federal de Sergipe. Creation of a genomic library for patchouli enabled the design of 12 microsatellite primers. Six of these microsatellites were polymorphic, revealing two well-defined groups of individuals that possess exclusive alleles. The data allowed us to characterize the patchouli active Germplasm Bank, identify its genetic diversity, and provide new information for researching this species.