Chemical characterization, antioxidant, cytotoxic and antibacterial activity of propolis extracts and isolated compounds from the Brazilian stingless bees<i>Melipona quadrifasciata</i>and<i>Tetragonisca angustula</i>

Santos, Larissa dos; Hochheim, Sabrina; Boeder, Ariela Maína; Kroger, Aline; Tomazzoli, Maíra Maciel; Neto, Remi Dal Pai; Maraschin, Marcelo; Guedes, Alessandro; Córdova, Caio Maurício Mendes de

doi:10.1080/00218839.2017.1371535

Cited by 38 publications

(27 citation statements)

References 62 publications

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“…However, it had a bacteriostatic effect against Gram-negative microorganisms, with MIC ranges from 0.6 to >5 mg/mL. These antimicrobial activity results are in line with other findings stating that Gram positive are sensitive to low propolis concentration and Gram-negative bacteria only inhibited with higher propolis dose [ 16 ]. This difference could be attributed to variable cell wall and membrane structure of the corresponding organisms.…”

Section: Discussionsupporting

confidence: 88%

“…The complex chemical composition of propolis relying upon the plant origin, geographical location, and the collection seasons. Our propolis contained more than 100 substances in different concentrations such as phenolics, flavonoids and alkaloids that are responsible for its biological and pharmaceutical properties [ 16 ]. Several studies concluded that Asian, African, and European propolis contains predominantly phenolics and flavonoids such as naringenin, galangin, pinocembrin, apigenin, pinobanksin, quercetin, cinnamic acid and its esters, kaempferol, chrysin, cinnamyl caffeate, cinnamylidene acetic acid, caffeic acid, p -coumaric acid, aromatic acids and their esters [ 17 , 18 ].…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial Activities of European Propolis Collected from Various Geographic Origins Alone and in Combination with Antibiotics

et al. 2018

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Background: Propolis consists of a complex mixture of resinous substances collected by honeybees from different plant sources. The objective of this study was to investigate the chemical composition, biological activities, and synergistic properties with antibiotics of propolis samples collected from various geographic origins (Germany, Ireland, and Czech Republic). Methods: The chemical composition of the propolis was analyzed by Gas Liquid Chromatography-Mass Spectrometry (GLC-MS) and High-performance liquid chromatography (HPLC). The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were evaluated by the standard broth microdilution method, while synergistic interactions were assessed by checkerboard dilution and time-kill curve assays. Results: HPLC and GLC-MS analyses revealed that ethanol extract of propolis (EEP) and water extracts of propolis (WEP) contained more than 100 different phytochemicals. The most abundant compounds were aromatic alcohols, aromatic acids, cinnamic acid and its esters, fatty acids, and flavanone (chrysin). Czech propolis showed the highest phenolic content (129.83 ± 5.9 mg CAE/g) followed by Irish propolis and German propolis. Furthermore, Irish propolis exhibited the highest value of total flavonoid content (2.86 ± 0.2 mg QE/g) and antioxidant activity (IC50 = 26.45 µg/mL). All propolis samples showed moderate antibacterial effect against Gram-positive microorganisms with MIC ranging from 0.08 mg/mL to 2.5 mg/mL. Moreover, EEP exhibited moderate activity against Gram-negative bacteria with MIC between 0.6 mg/mL to 5 mg/mL. In addition, EEP displayed moderate antifungal activity (MIC values between 0.6–2.5 mg/mL). The results obtained from time kill-kinetic assay and checkerboard dilution test of two-drug combinations between EEP and antibiotics such as vancomycin, oxacillin, and levofloxacin indicate mainly synergistic interactions against drug-resistant microbial pathogens including MRSA and VRE. Conclusions: The propolis extract synergistically enhanced the efficacy of antibiotics, especially those acting on cell wall synthesis (vancomycin and oxacillin) against drug-resistant microorganisms.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activities of European Propolis Collected from Various Geographic Origins Alone and in Combination with Antibiotics

et al. 2018

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“…Extracts of this propolis type have been found to contain phenols, tannins, flavones, flavonols, xanthones and catechins, as well as saponins, triterpenes and steroids. 22 Terpenes have already been studied as antioxidants capable of stopping the free radical cascade formation. 20 Compared to commercially available standards of 14 phenolic substances, quercetin, epigallocatechin, p-OH-benzoic acid, epigallocatechin gallate and coumaric acid could be identified by HPCL in the hydroalcoholic extract, 22 as could catechin, epicatechin, aromadendrin, naringenin, pinocembrin and pcoumaric acid by HPCL-ESI-MS/MS.…”

Section: Discussionmentioning

confidence: 99%

“…22 Terpenes have already been studied as antioxidants capable of stopping the free radical cascade formation. 20 Compared to commercially available standards of 14 phenolic substances, quercetin, epigallocatechin, p-OH-benzoic acid, epigallocatechin gallate and coumaric acid could be identified by HPCL in the hydroalcoholic extract, 22 as could catechin, epicatechin, aromadendrin, naringenin, pinocembrin and pcoumaric acid by HPCL-ESI-MS/MS. 23 The chemical and compound interactions resulting from the addition of propolis to beer deserve to be better studied, especially regarding terpene content.…”

Section: Discussionmentioning

confidence: 99%

“…20 In a previous study with samples of propolis from the native bee Melipona quadrifasciata, researchers created a profile for the aqueous and hydroalcoholic extracts of the green/yellow propolis that included phenols, tannins, flavonoids, steroids, triterpenes, flavones, flavonols and xanthones. 22 Recent studies have shown the antioxidant potential of different fractions of hydroalcoholic extracts and the methanol extract of Melipona quadripasciata propolis. The reducing potential, in ascorbic acid equivalents per 100 g of sample, was best with the ethyl acetate fraction (127.83 ± 1.45 mg AA 100 g-1), followed by the methanolic extract and the insoluble fraction.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant Potential of the Propolis of Melipona Quadrifasciata as a Preservative for Craft Beer

Galgowski¹,

Fischer²,

Simionatto³

et al. 2019

Rev. Virtual Quim.

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Potencial Antioxidante da Própolis de Melipona Quadrifasciata como Conservante de Cerveja Artesanal Resumo: Propolis é uma mistura complexa estudada devido à suas propriedades biológicas, mas frequentemente aquela produzida pela abelha Apis melifera. Poucos estudos foram realizados, proporcionalmente, com própolis de abelhas nativas brasileiras, uma vez que se sabe que a composição química da própolis varia de acordo com a espécie de abelha que a produz. O potencial antioxidante do extrato de própolis da abelha Melipona quadripasciata já foi demonstrado. Apesar do seu relativamente baixo teor de compostos fenólicos e flavonóides, tem a capacidade de redução do potencial e inibição do radical livre DPPH e inibição da peroxidação lipídica. Assim, buscou-se avaliar o potencial de diferentes frações do extrato hidroalcoólico dessa própolis de abelha nativa incorporada em amostras de cerveja de baixa fermentação, para determinar se poderia eventualmente aumentar a vida de prateleira da bebida, melhorando sua qualidade. Os compostos da própolis foram extraídos com solventes de diferentes polaridades, secos e adicionados às amostras de cerveja em diferentes concentrações. Todas as amostras adicionadas de própolis inibiram a oxidação sem alterar as características organolépticas da cerveja. O uso de própolis como conservante de cervejas artesanais brasileiras, uma vez bem fundamentada, pode agregar valor significativo à cadeia produtiva da apicultura no país. Notavelmente, os compostos extraídos na fração diclorometano deste tipo de própolis parecem ser promissores, uma vez que até melhoram a percepção da cerveja. As interações químicas e compostos resultantes da adição de própolis à cerveja merecem ser melhor estudados.

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Mollicute Anti‐Adhesive and Growth Inhibition Properties of the Methanolic Extract of Propolis from the Brazilian Native Bee Melipona quadrifasciata

Galgowski

Frare

Rau

et al. 2020

Chemistry & Biodiversity

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Hydroalcoholic propolis extracts from the bee species Melipona quadrifasciata have been shown to possess antimicrobial activity against different mollicute strains, but a methanolic extract (ME) could contain an increased diversity of nonpolar bioactive components with a potentially higher antimicrobial activity. The ME obtained by maceration of the propolis sample was fractionated with solvents of different polarities and then, purified by silica gel column chromatography through biomonitoring of its antimicrobial activity against mollicute strains. Analysis by gas chromatography-mass spectrometry (GC/MS) enabled the identification of compounds using the NIST library. Minimum inhibitory concentrations (MICs) of the samples were determined by broth microdilution. Anti-adhesive assays were performed with Mycoplasma pneumoniae cells. The hexane (MIC = 62.5 mg/L) and dichloromethane (MIC = 125 mg/L) fractions presented the most promising results against M. pneumoniae. They were fractionated into 74 subfractions, and even the best ones did not show better results (MIC > 250 mg/L) than their original fractions, likely due to the loss of terpene compounds that seem to act in synergy. The dichloromethane subfraction FD4 was highlighted in the anti-adhesive assay with an inhibitory activity of 21.6 %. A synergistic effect of the nonpolar compounds in M. quadrifasciata propolis may be responsible for its antibacterial activity, but several purified components can improve its anti-adhesive properties.

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Chemical characterization, antioxidant, cytotoxic and antibacterial activity of propolis extracts and isolated compounds from the Brazilian stingless beesMelipona quadrifasciataandTetragonisca angustula

Cited by 38 publications

References 62 publications

Antimicrobial Activities of European Propolis Collected from Various Geographic Origins Alone and in Combination with Antibiotics

Antimicrobial Activities of European Propolis Collected from Various Geographic Origins Alone and in Combination with Antibiotics

Antioxidant Potential of the Propolis of Melipona Quadrifasciata as a Preservative for Craft Beer

Mollicute Anti‐Adhesive and Growth Inhibition Properties of the Methanolic Extract of Propolis from the Brazilian Native Bee Melipona quadrifasciata

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