Chemical Characteristics of Salvia miltiorrhiza (Danshen) Collected from Different Locations in China

Abstract

The chemical characteristics of Salvia miltiorrhiza , also called "danshen" in China, were investigated on the basis of the simultaneous quantitative determination of 13 hydrophilic and lipophilic compounds, namely, protocatechuic acid, protocatechuic aldehyde, caffeic acid, ferulic acid, isoferulic acid, rosmarinic acid, salvianolic acid B, salvianolic acid A, dihydrotanshinone I, przewalskin, cryptotanshinone, tanshinone I, and tanshinone IIA, in 74 samples collected from different locations using ultraperformance liquid chromatography (UPLC). Hierarchical clustering analysis based on 13 investigated compounds and the similarity of the entire chromatographic pattern showed that S. miltiorrhiza was significantly different from Salvia przewalskii , an adulterant of danshen. The chemical characteristics of S. miltiorrhiza collected from different locations in China were revealed, and salvianolic acid B, rosmarinic acid, cryptotanshinone, and tanshinones I and IIA were optimized as markers for the evaluation, which is helpful in the quality control of S. miltiorrhiza.

UPLC/Q-TOF-MS Analysis for Identification of Hydrophilic Phenolics and Lipophilic Diterpenoids from Radix Salviae Miltiorrhizae

“…Pharmacol-ogical studies established that these compounds possessed anti-ischemic, antioxidant, and antitumor activities [9,10]. Some analytical techniques were utilized for separation and identification of constituents in RSM, such as high-performance thin-layer chromatography (HPTLC), high-performance liquid chromatography (HPLC), ultra-performance liquid chromatography (UPLC), and liquid chromatography hyphenated with tandem mass spectrometry (LC-MS/MS) [11][12][13]. However, HPTLC had a disadvantage of low resolution, which resulted in inaccuracy.…”

Functional divergence of diterpene syntheses in the medicinal plant Salvia miltiorrhiza Bunge

“…The lipophilic pigments from the reddish root and rhizome consist of abietane quinone diterpenoids (Nakao and Fukushima, 1934), largely tanshinone IIA, cryptotanshinone, and tanshinone I (Zhong et al, 2009). These are highly bioactive.…”

High-performance liquid chromatography fingerprints and simultaneous quantification of bioactive compounds in Salvia przewalskii Maxim

“…Similarity was analyzed by the Chromatographic Fingerprint of Traditional Chinese Medicine software (Version 2004A), which was developed by the Chinese Pharmacopoeia Committee [12,21]. This software evaluates similarity based on correlative coefficient calculations for fingerprint chromatograms.…”

“…The stability was tested by analyzing the same sample solution after being prepared in 0, 2,4,6,8,12, and 24 h. The RSD values of the eight standards ranged from 1.6% to 3.3%. The data revealed that the stability of the solution was acceptable.…”

“…The quality of plant medicine mainly depends on the complex secondary metabolites, including the constituents and content. Due to different cultivation areas, climatic conditions and other factors, its chemical constituents and content may vary significantly, which makes it difficult to maintain consistency of the quality [12,13]. S. przewalskii is wildly distributed in the western plateau regions of China [14].…”