UPLC/Q-TOF-MS Analysis for Identification of Hydrophilic Phenolics and Lipophilic Diterpenoids from Radix Salviae Miltiorrhizae

Abstract

Summary.A rapid method has been used for simultaneous identification of both hydrophilic and lipophilic compounds from Radix Salviae Miltiorrhizae (RSM, the root of Salvia miltiorrhiza B GE .) by ultra-performance liquid chromatography/quadrupole time-offlight mass spectrometry (UPLC/Q-TOF-MS). A total of 58 compounds extracted by methanol were detected and tentatively identified within 20 min, including hydrophilic phenolics, lipophilic diterpenoids, a verbascose, and several organic acids. These compounds were separated on an Acquity UPLC BEH C18 column and identified based on tandem mass spectrometry (MS/MS) fragmentation patterns under the positive and negative ion modes, respectively. Among them, micranthin B and 9-oxo-10E,12Z-octadecadienoic acid were reported in RSM for the first time. Their fragmentation patterns in electrospray ionization (ESI)-MS/MS spectra were first investigated by matching their accurate molecular masses. This contribution presented one of the first reports on the analysis of hydrophilic phenolics and lipophilic diterpenoids from Radix Salviae Miltiorrhizae using UPLC/Q-TOF-MS. The results demonstrated that UPLC/Q-TOF-MS method could be applied to rapidly and expediently describe and provide comprehensive chemical information for simultaneous analysis of two different polar components in RSM.

Analysis of Polyphenolic Composition of a Herbal Medicinal Product—Peppermint Tincture

“…In addition to caffeic acid (peak 3) and rosmarinic acid (peak 21) this research has shown the presence of ten other caffeic acid esters (peaks 1,7,15,19,22,24,25,27,30,34) known as lithospermic acids or salvianolic acids. Caffeic acid, rosmarinic acid, lithospermic acid A (peak 22), and lithospermic acid B (peak 24) were identified using authentic standards or herbal reference materials (Salvia miltiorrhiza Bunge, Melissa officinalis L.) and literature data [32,34,35].…”

Quality transitivity of Danhong Huayu Koufuye: A study on chemical profiles of medicinal herbs, compound preparation and dosed rat plasma using ultra‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry

“…Applying the optimized LC–MS conditions, samples of the seven medicinal herbs were analyzed and typical base peak ion chromatograms are shown in Figure S1. Comparing the MS spectra with standards and literature data (Chen et al, ; Chen, Qi, Chang, Zhu, & Yu, ; Hong, Wang, Xu, Li, & Li, ; Liang et al, ; Liu et al, ; Ma et al, ; Si et al, ; Yang et al, ; Yang, Wu, Rui, Guo, & Feng, ; Yi, Leung, Lu, Chan, & Zhang, ), a total of 134 chemical constituents were identified in the seven medicinal herbs, and their information is summarized in Tables S1–S7. Among these, 31 compounds in Salviae Miltiorrhizae were mainly salvianolic acids and diterpene quinones, 16 compounds in Radix Angelica Sinensis and 26 compounds in Rhizoma Chuanxiong were mostly phenolic acids and phthalides, three compounds in Semen Persicae were mainly cyanogenic glycosides, 16 compounds in Flos Carthami and 18 compounds in Fructus Aurantii were largely flavonoids, and 24 compounds in Radix Bupleuri were mostly triterpenoid saponins.…”

Hybrid multidimensional data acquisition and data processing strategy for comprehensive characterization of known, unknown and isomeric compounds from the compound Dan Zhi Tablet by UPLC-TWIMS-QTOFMS

“…A total of 35 salvianolic acids were identied, of which ve compounds (danshensu, lithospermic acid, salvianolic acid A, salvianolic acid B and rosmarinic acid) were unambiguously identied by comparing their retention times, accurate molecular masses and MS/MS fragments with those of the standard references; moreover, the remaining 30 compounds were tentatively identied by comparing their exact molecular masses, fragmentation information, retention times and dri times with previously reported values. [32][33][34] Identication of astragalosides in DZT. Most of the astragalosides contain the same tetracyclic triterpenoids , corresponding to the aglycone and successive losses of water molecules; thus, these ions can help us identify as many astragalosides as possible.…”