Chemical characteristics of an Ilex Kuding tea polysaccharide and its protective effects against high fructose-induced liver injury and vascular endothelial dysfunction in mice

Abstract

The present study was designed to investigate the protective effects of Ilex Kuding tea polysaccharides (IKTP) on high fructose (HF)-induced liver injury and vascular endothelial dysfunction in mice. IKTP were identified as acidic heteropolysaccharides by FT-IR and HPLC. Healthy male Kunming mice fed 20% fructose in drinking water for 8 consecutive weeks significantly displayed dyslipidemia, hepatic steatosis, oxidative stress and vascular endothelial dysfunction. However, continuous administration of IKTP at 200, 400 and 800 mg per kg bw in HF-fed mice could prevent the damage caused by HF-diets, especially at dosages of 400 and 800 mg per kg bw (p < 0.01). IKTP significantly reduced the HF-induced elevation of the serum TC, TG, LDL-C, TXA and ET-1 levels, as well as AST and ALT activities, while markedly increased the HF-induced decline of HDL-C, PGI and eNOS levels in the serum compared to HF-fed mice. Meanwhile, the hepatic MDA level was lowered while SOD and GSH-Px activities were increased in IKTP-treated mice, compared to HF-fed mice. Histopathology of the liver and cardiovascular aortic by H&E or oil red O staining confirmed the liver steatosis and the vascular injury induced by HF-diets and the protective effects of IKTP. These findings suggest that HF causes oxidative damage, and IKTP alleviates liver injury and vascular endothelial dysfunction.

Acute Exposure to Fructose Impairs Endothelium-Dependent Relaxation via Oxidative Stress in Isolated Rat Aortic Rings

“…High-fructose intake is well known for inducing oxidative stress [14][15][16]. To improve or prevent hypertension, antioxidants are used as a treatment in vivo [8][9][10] and as pretreatment in vitro [12,13].…”

Consumption of post-fermented Jing-Wei Fuzhuan brick tea alleviates liver dysfunction and intestinal microbiota dysbiosis in high fructose diet-fed mice

“…[26][27][28] Interestingly, it is claimed that drinking tea has a potential role in the treatment and prevention of liver injury caused by HFD. [29][30][31] A special Jing-Wei Fuzhuan brick tea is a unique post-fermented tea which is distinguished from the other post-fermented teas by deliberate fermentation with the probiotics Eurotium cristatum, and exhibits a variety of biological activities, such as antiobesity, antibacterial, and anti-oxidation effects. [12][13][14]32 A few reports have mainly focused on the investigation on anti-obesity and anti-oxidation properties of Fuzhuan brick tea, and limited study on the inuence of Fuzhuan brick tea against NAFLD is performed, and the regulatory effect of Fuzhuan brick tea on intestinal microbiota was also rarely studied.…”

Health Benefits of Bioactive Compounds from the Genus Ilex, a Source of Traditional Caffeinated Beverages

“…Kudingcha extract showed ameliorative effects on blood vessel contractility and blood flow in both rats and rabbits [ 3 ]. Kudingcha polysaccharides were also reported to have a protective effect against vascular dysfunction in high fructose-fed mice [ 86 ]. Yerba Mate consumption had great potential for reducing intermediate factors for cardiovascular diseases in both animal and human interventional studies [ 87 ].…”